현탁배양을 통한 음나무(Kalopanax pictus) 배발생 캘러스의 증식
Proliferation of Embryogenic Callus of Kalopanax pictus through Suspension Culture System
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Kalopanax pictus was cultured in vitro to find out optimal condition for embryogenic cells proliferation in liquid media rapidly. Embryogenic cells were induced from leaves and petiols of Kalopanax pictus. Optimum culture medium appeared to be a 1/2MS medium supplemented with 2.0㎎/L 2,4-D and 0.1㎎/L BA. To find out optimal conditions, embryogenic cells were cultured some condition as different concentrations of 2,4-D, medium and sucrose. There was cultured on 1/2MS liquid medium containing different concentration of 2,4-D. When embryogenic cells were cultured on 1/2MS liquid medium supplemented with 1.0㎎/L 2,4-D, cell propagation rate was higher than other concentration of 2,4-D. When embryogenic cells were cultured on different media that MS, Gamborg B5, N6, White, SH medium, observed the highest multiplication rate among Gamborg B5 and White medium. To find out of effect of sucrose to embryogenic cells propagation, we tested cells under different concentrations. Optimal concentration of sucrose appeared to be a basal medium added 3% sucrose. Above results suggest that optimal conditions for proliferation of embryogenic cells were established Gamborg B5 and White medium added 1.0㎎/L 2,4-D and 3% sucrose. There is every possibility achieving embryogenic cells proliferation via bioreactor culture system in Kalopanax pictus.
Abstract
Kalopanax pictus was cultured in vitro to find out optimal condition for embryogenic cells proliferation in liquid media rapidly. Embryogenic cells were induced from leaves and petiols of Kalopanax pictus. Optimum culture medium appeared to be a 1/2MS medium supplemented with 2.0㎎/L 2,4-D and 0.1㎎/L BA. To find out optimal conditions, embryogenic cells were cultured some condition as different concentrations of 2,4-D, medium and sucrose. There was cultured on 1/2MS liquid medium containing different concentration of 2,4-D. When embryogenic cells were cultured on 1/2MS liquid medium supplemented with 1.0㎎/L 2,4-D, cell propagation rate was higher than other concentration of 2,4-D. When embryogenic cells were cultured on different media that MS, Gamborg B5, N6, White, SH medium, observed the highest multiplication rate among Gamborg B5 and White medium. To find out of effect of sucrose to embryogenic cells propagation, we tested cells under different concentrations. Optimal concentration of sucrose appeared to be a basal medium added 3% sucrose. Above results suggest that optimal conditions for proliferation of embryogenic cells were established Gamborg B5 and White medium added 1.0㎎/L 2,4-D and 3% sucrose. There is every possibility achieving embryogenic cells proliferation via bioreactor culture system in Kalopanax pictus.
참고문헌 (24)
- Choi, J. W., "Kalopanaxsaponin A from Kalopanax pictus, a potent antioxidant in the rheumatoidal in the rheumatoidal rat treated with Freund's complete adjuvant reagent", J. Ethmopharmacol, 79, pp.113~118 (2002)
- Choi, J. W., "Actinociceptive and anti-rheumatoidal effects of Kalopanax pictus extract and its saponin components in experimental animals", J. Ethmopharmacol, 79, pp.199~204 (2002)
- Choi, Y. E., "High frequency of plant production via somatic embyogenesis from callus or cell suspension cultures in Eleutherococcus senticosus", Ann. Bot, 83, pp.309~314 (1999)
- Choi. Y. E., "Production of plantlets of Eleutherococcus sessiliflorus via somatic embryogenesis and successful transfer to soil", Plant Cell Tiss. Organ Cult, 69, pp.201~204 (2002)
- Choi, T. E., "Rapid propagation of Eleutherococcus senticosus via direct somatic embryogenesis from explants of germinating zygotic embryo", Plant Cell Tiss. Organ Cult, 58, pp.93~97 (1999)
- Choi, T. E., "Highefficiency plant production via direct somatic single embryogenesis from preplasmolysed cotyledons of Panax ginseng and possible dormancy of somatic embryos", Plant Cell Rep, 18, pp.493~499 (1999)
- Fuentes, S. R. L., "The effectos of silver mitrate and different carbohydrate sources on somatic embryogenesis in Coffea canephora", Plant Cell Tiss. Org. Cult, 60, pp.5~13 (2000)
- "Plant propagation by tissue culture. Part 1. The Technology" (1993), Exegetics Ltd.
- Gui, Y., "Somatic embryogenesis and plant regeneration in Acanthopanax senticosus", Plant Cell Rep, 9, pp.514~516 (1991)
- Hernandez, I., "Vegetative propagation of Quercus suber L. by somatic embryogenesis: Ⅱ. Plant regeneration from selected cork oak trees", Plant Cell Rep, 21, pp.765~770 (2003)
- Hilae, A, "Effects of carbon sources and strength of MS medium on germination of somatic embryo of oil palm(Elaeis quineensis Jacq.) Songklanakarin", J. Sci. Technol, 27, pp.629~635 (2005)
- Karhu, S. T, "Sugar use in relation to shoot induction by sorbitol and cytokinin in apple", J. Am. Soc. Hort. Sci, 122, pp.476~480 (1997)
- Kim, Y. K., "In vitro anti-inflammatory activity of kalopanaxsaponin A isolated from Kalopanax pictus in murine macrophage RAW 264.7 cells", Biol. Pharm. Bull, 24(4), pp.472~476 (2002)
- Kim, Y. K., "In vitro anti-inflammatory activity of kalopanaxsaponin A isolated from Kalopanax pictus in murine macrophage RAW 264.7 cells", Biol. Pharm. Bull, 25(4), pp.472~476 (2002)
- Mamiya, K, "Effect of concentration and strength of basal medium on conversion of somatic embryos on Asparagus offcinalis L", Sci Hort, 84, pp.15~26 (2000)
- Moon, H. K., "Micropropagation of Kalopanax pictus tree via somatic embryogenesis", 41, pp.303~306 (2005)
- Park, H. J., "A potent antidiabetic agent from Kalopanax pictus", Arch. Pharm. Res, 21, pp.24~29 (1998)
- Petersen, K. K., "Significance of different carbon sources and sterilization methods on callus induction and plant regeneration of Miscanthus×ogiformis Honda Giganteus", Plant Cell Tiss. Org. Cult, 58, pp.189~197 (1999)
- Ramarosandratana, A., "Effects of carbohydrate source, polyethylene glycol and gellan fum concentration on embryonal-suspensor mass (ESM) proliferation and maturation of maritime pine somatic embryos", In Vitro Cell. Dev. Biol.-Plant, 37, pp.29~34 (2001)
- Sano, K., "Studies on the constituents of the stem bark of Kalopanax pictus Nakai", Chem. Pharm. Bull, 39, pp.865~870 (1991)
- Scott, P., "Metabolismof maltose and sucrose by microspores isolated from barley (Hordeum vulgare)", Planta, 197, pp.435~441 (1995)
- Tachikawa. Y., "Changes in protein pattern during stress induction of carrot (Daucus carota L.) somatic embryogenesis", Plant Biotechnol, 15, pp.17~22 (1998)
- Yeoung, Y. R., "Seed germination and softwood cutting technique of Kalopanax pictus Nakai", Kor J. Plant Resources, 14, pp.53~59 (2001)
- 채영복, "한국유용식물자원 연구편람", 한국화학연구소, 122~ (1988)