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발아 메밀 추출물의 항산화·항균활성 및 세포독성

Antioxidative, Antimicrobial and Cytotoxic activities of Fagopyrum esculentumM？ench Extract in Germinated Seeds

황은주
한국약용작물학회|2006.02|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제14권 제1호|pp. 1-7

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Abstract

This research was conducted to investigate the possibilities of usage of germinated-buckwheat (Fagopyrum esculentum Moench) by examining antioxidative, antimicrobial and cytotoxic effects of extracts from different germinated root length of buckwheat. Antioxidant activity (RC50) was shown higher in extracts of non-germinated seed (50.41 ㎍/mL) and root length 10 ㎜ (80.57 ㎍/mL), 2 ㎜ (93.77 ㎍/mL), 5 ㎜ (107.09 ㎍/mL) than BHT (163.96 ㎍/mL) as a synthetic antioxidant. In antimicrobial activity, non-germinated and germinated seeds were formed inhibitory zone against S. aureus (4~10 ㎜), P. aeruginosa (2~9㎜) at the concentrations of 10~40 ㎎/mL but B. subtilis, E. coli and S. typhimurium were not apparent antimicrobial activity. Extracts of germinated seed also decreased their antimicrobial activity compared to nongerminated seed extract. In addition, the growth of Calu-6 was inhibited of both 5 ㎜ root length germinated and non-germinated seeds (800 ㎍/mL) as 95.12% and 87.15%, respectively, but these did not show any influence on cytotoxic effect against MCF-7 and Caco-2 cell lines. Extracts of 2 ㎜ and 5 ㎜ germinated seeds were also inhibited against Calu-6 and SNU-601 cell lines.

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