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Inhibitory Effect of Chaenomeles sinensis Fruit on Amyloid β Protein (25-35)-Induced Neurotoxicity in Cultured Neurons and Memory Impairment in Mice

성연희|정명환|송경식
한국약용작물학회|2012.02|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제20권 제1호|pp. 8-15

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참고문헌

키워드

Memory ImpairmentNeuroprotectionAmyloid β ProteinCultured NeuronsChaenomeles sinensis Fruit

초록

The present study investigated an ethanol extract of Chaenomeles sinensis fruit (CSF) for possible neuroprotective effects on neurotoxicity induced by amyloid β protein (Aβ) (25-35) in cultured rat cortical neurons and also for antidementia activity in mice. Exposure of cultured cortical neurons to 10 μM Aβ (25-35) for 36 h induced neuronal apoptotic death. At 0.1-10㎍/㎖, CSF inhibited neuronal death, elevation of intracellular calcium concentration ([Ca2+]i), and generation of reactive oxygen species (ROS) induced by Aβ (25-35) in primary cultures of rat cortical neurons. Memory loss induced by intracerebroventricular injection of mice with 15 nmol Aβ (25-35) was inhibited by chronic treatment with CSF (10, 25 and 50 ㎎/㎏, p.o. for 7 days) as measured by a passive avoidance test. CSF (50 ㎎/㎏) inhibited the increase of cholinesterase activity in Aβ (25-35)-injected mice brain. From these results, we suggest that the antidementia effect of CSF is due to its neuroprotective effect against Aβ (25-35)-induced neurotoxicity and that CSF may have a therapeutic role for preventing the progression of Alzheimer’s disease.

Abstract

The present study investigated an ethanol extract of Chaenomeles sinensis fruit (CSF) for possible neuroprotective effects on neurotoxicity induced by amyloid β protein (Aβ) (25-35) in cultured rat cortical neurons and also for antidementia activity in mice. Exposure of cultured cortical neurons to 10 μM Aβ (25-35) for 36 h induced neuronal apoptotic death. At 0.1-10㎍/㎖, CSF inhibited neuronal death, elevation of intracellular calcium concentration ([Ca2+]i), and generation of reactive oxygen species (ROS) induced by Aβ (25-35) in primary cultures of rat cortical neurons. Memory loss induced by intracerebroventricular injection of mice with 15 nmol Aβ (25-35) was inhibited by chronic treatment with CSF (10, 25 and 50 ㎎/㎏, p.o. for 7 days) as measured by a passive avoidance test. CSF (50 ㎎/㎏) inhibited the increase of cholinesterase activity in Aβ (25-35)-injected mice brain. From these results, we suggest that the antidementia effect of CSF is due to its neuroprotective effect against Aβ (25-35)-induced neurotoxicity and that CSF may have a therapeutic role for preventing the progression of Alzheimer’s disease.

참고문헌 (44)

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관련 논문AI추천

Ethanol Extract of Three Plants of Curcuma longae Radix, Phellinus linteus, and Scutellariae Radix Inhibits Amyloid β Protein (25-35)-Induced Neurotoxicity in Cultured Neurons and Memory Impairment in Mice김주연, 반주연, 배기환, 성연희, 유재국, 정하연한국약용작물학회 · 한국약용작물학회지2009 · 제17권 제6호Moutan Cortex Extract Inhibits Amyloid β Protein (25-35)-induced Neurotoxicity in Cultured Rat Cortical Neurons김주연, 반주연, 성연희, 송경식, 주현수한국약용작물학회 · 한국약용작물학회지2008 · 제16권 제6호Inhibitory Effect of an Ethanol Extract Mixture of Vitis amurensis, Aralia cordata, and Glycyrrhizae radix on Amyloid β Protein (25-35)-Induced Neurotoxicity성연희, 장지연한국약용작물학회 · 한국약용작물학회지2014 · 제22권 제2호Protective Effect of Sanguisorba officinalis L. Root onAmyloid β Protein (25-35)-induced Neuronal CellDamage in Cultured Rat Cortical Neuron배기환, 성연희, 송경식, Ju Yeon Ban, Soon Ock Cho, So Young Jeon한국약용작물학회 · 한국약용작물학회지2005 · 제13권 제5호Ameliorative Effect of Aster scaber Thunberg and Chaenoleles sinensis Koehne Complex Extracts Against Oxidative Stress-induced Memory Dysfunction in PC12 Cells and ICR Mice박찬규, 신동훈, 최수정한국약용작물학회 · 한국약용작물학회지2019 · 제27권 제6호Effects of the fermented Zizyphus jujuba in the amyloid β25-35-induced Alzheimer's disease mouse model조은주, Jung Ji Eun, Kim Hyun Young, KIM, MIN-JEONG, Lee Sanghyun한국영양학회 · Nutrition Research and Practice2021 · 제15권 제2호

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