메티실린-내성 포도상구균에 대하여 Brazilein 혼합에 따른 항생제 Hygromycin-b의 상승효과
Synergistic Effect of Brazilein in Combination with Hygromycin-b against Staphylococcus aureus
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Methicillin-resistant Staphylococcus aureus (MRSA) is a substantial contributor to morbidity and mortality. In search of a natural products capable of inhibiting this multidrug resistant bacteria, we have investigated the antimicrobialactivity of brazilein (BRZ) isolated from Caesalpinia sappan L. (Leguminosae) against 8 different strains of Staphylococcusaureus (S. aureus). New antimicrobial activity was found using the minimum inhibitory concentrations (MICs), brothdilution as well as checkerboard method. Against the 8 strains, the minimum inhibitory concentrations of BRZ were in therange of 62.5-500㎍/mL. From those results we performed the checkerboard test to determine the synergism of BRZ incombination with Hygromycin-b (HgB) against 4 strains. The combined activity of BRZ and HgB against 4 strains resultedin a fractional inhibitory concentrations index (FICI) ranging from 0.18-0.5. The effect of BRZ with HgB was found to besynergistic. We found that BRZ reduced the MICs of HgB. BRZ and HgB could lead to the development of new combinationantibiotics against MRSA infection.
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is a substantial contributor to morbidity and mortality. In search of a natural products capable of inhibiting this multidrug resistant bacteria, we have investigated the antimicrobialactivity of brazilein (BRZ) isolated from Caesalpinia sappan L. (Leguminosae) against 8 different strains of Staphylococcusaureus (S. aureus). New antimicrobial activity was found using the minimum inhibitory concentrations (MICs), brothdilution as well as checkerboard method. Against the 8 strains, the minimum inhibitory concentrations of BRZ were in therange of 62.5-500㎍/mL. From those results we performed the checkerboard test to determine the synergism of BRZ incombination with Hygromycin-b (HgB) against 4 strains. The combined activity of BRZ and HgB against 4 strains resultedin a fractional inhibitory concentrations index (FICI) ranging from 0.18-0.5. The effect of BRZ with HgB was found to besynergistic. We found that BRZ reduced the MICs of HgB. BRZ and HgB could lead to the development of new combinationantibiotics against MRSA infection.
참고문헌 (23)
- Aqil F, "Effect of certain bioactive plant extracts on clinical isolates of beta-lactamase producing methicillin resistant Staphylococcus aureus", Journal of Basic Microbiology, 45, pp.106~114 (2005)
- 최장기, "In Vitro Activity of Methyl Gallate Isolated from Galla Rhois Alone and in Combination with Ciprofloxacin Against Clinical Isolates of Salmonella", Journal of Microbiology and Biotechnology, 18(11), pp.1848~1852 (2008), 한국미생물·생명공학회
- Clinical and Laboratory Standards Institute(CLSI)., "Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved standard(9th Ed.)", pp.M7~A5 (2012), Clinical and Laboratory Standards Institute
- 음원식, "TPA로 유도된 마우스 귀 부종 동물모델에서 소목추출물의 항염증 효과", 한국식품영양과학회지, 42(3), pp.384~388 (2013), 한국식품영양과학회
- Fu Y, "Antimicrobial activity of clove and rosemary essential oils alone and in combination", Phytotherapy Research, 21, pp.989~994 (2007)
- 이정훈, "한국산 개똥쑥의 재배특성 및 수확시기에 따른 Artemisinin의 함량변이", 한국약용작물학회지, 21(6), pp.433~438 (2013), 한국약용작물학회[DOI]
- 이재훈, "가공식품과 비가공식품에서의 황색포도상구균 검출을 위한 배지법과 Real-time PCR법의 비교", 한국축산식품학회지, 30(3), pp.410~418 (2010), 한국축산식품학회
- 이주영, "식품에서 분리한 황색포도상구균의 항생제 내성 특징 및 균막 형성", 한국미생물·생명공학회지, 41(1), pp.96~104 (2013), 한국미생물·생명공학회[DOI]
- 장설, "유통 한약재의 중금속 잔류실태 조사", 한국약용작물학회지, 20(6), pp.434~439 (2012), 한국약용작물학회[DOI]
- Kalivoda EJ, "New vector tools with a hygromycin resistance marker for use with opportunistic pathogens", Molecular Biotechnology, 48, pp.7~14 (2011)
- 강태윤, "Prevention of Salmonella Infection in Layer Hen Fed with Microbial Fermented Citrus Shell", 생명과학회지, 20(2), pp.190~196 (2010), 한국생명과학회
- AaronKettle, "소음성 난청 및 이독성 난청의 예방을 위한 항산화제 치료", 대한이비인후과학회지 두경부외과학, 45(10), pp.931~935 (2002), 대한이비인후과학회
- Kim DS, "NMR assignment of brazilein", Phytochemistry, 46, pp.177~178 (1997)
- 김에스더, "黃芩芍藥湯의 MRSA에 대한 항균활성에 관한 연구", 한방재활의학과학회지, 23(3), pp.15~26 (2013), 한방재활의학과학회
- 김애란, "신생아에서의 Methicillin-resistant Staphylococcus aureus (MRSA) 감염", Neonatal medicine, 20(3), pp.352~358 (2013), 대한신생아학회
- Kim KM, "The nationwide surveillance results of nosocomial infections along with antimicrobial resistance in intensive care units of sixteen university hospitals in Korea, 2004", Korean Journal of Nosocomial Infection Control, pp.79~86 (2006)
- 김남영, "다양한 적송잎 추출물의 항생제 내성 황색포도상구균에 대한 항균활성 검증", 생명과학회지, 20(4), pp.589~596 (2010), 한국생명과학회
- 김수정, "메티실린 내성 황색포도알균 판독에 있어 항균제 감수성 검사와 mecA PCR법의 비교", 미생물학회지, 47(4), pp.381~385 (2011), 한국미생물학회
- Kim SK, "Identification and antimicrobial susceptibility aspects of pathogenic Staphylococcus aureus", The Journal of the Korean Society for Microbiology, 28, pp.251~259 (1993)
- 조성기, "감마선 조사에 의한 소목추출물의 색상변화 및 항산화 활성 평가", 한국식품영양과학회지, 36(8), pp.1055~1061 (2007), 한국식품영양과학회
- Leclercq R, "Plasmid mediated resistance to vancomyhcin and teicoplanin", New England Journal of Medicine, 319, pp.157~161 (1998)
- Pfister P, "Role of 16S rRNA helix 44 in ribosomal resistance to hygromycin B", Antimicrobial Agents and Chemotherapy, 47, pp.1496~1502 (2003)
- 성인화, "백작약 추출물의 Methicillin-Resistant Staphylococcus aureus에 대한 항균활성", 미생물학회지, 42(1), pp.54~58 (2006), 한국미생물학회