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Identification of Entomopathogenic Fungus, Beauveria spp. F-101 Isolated from Thecodiplosis japonensis Using Internal Transcribed Spacer Sequence

김철수|박인권|제연호|노종열|전문장|신상철
한국잠사학회 (Korean Society Of Sericultural Science)|2004.03|International Journal of Industrial Entomology and Biomaterials|제8권 제1호|pp. 77-80

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Abstract

For the development of the alternative control system against the major forest pests, Beauveria spp. F-101, isolated from a dead larva of Thecodiplosis japonensis, was selected because this isolate showed high pathogenicities against T. japonensis and Acantholyda parki. Beauveria spp. F-101 had irregular clustered conidiophores and conidia borne on a distinctive apical zigzag extension, and it showed typical characteristic of the genus, Beauveria in morphology. For molecular basedidentification, the ribosomal ITS region of Beauveria spp. F-101 was amplified with ITS1 and ITS4 primers, and cloned into pGEM-T Easy vector. The amplified PCR product was 569 bp in size and completely sequenced. The similarities of the cloned ITS sequence were 99% and 97% to those of B. bassiana and B. brongniartii, respectively. In comparison to other species among the genus Beauveria, the ITS region of Beauveria spp. F-101 showed a similarity of 95% to B. amorpha, 95% to B. tenella, 89% to B. vermiconia and 69% to B. alba, respectively. In addition, in comparison to different genus, it had 95% similarities to Cordyceps militaris and 91% to Paecilomyces tenuipes. Accordingly, the current result suggests that Beauveria spp. F-101 was a variant of B. bassiana and it seems to be a new isolate considering sequence variation in ITS region.

참고문헌 (16)

  1. "Studies on the effects ofblack-tipped sawfly Acantholyda posticalis posticalis Mat-sumura on the growth of the Korean white pine", pp.450~459 (1994)
  2. "A taxo- nomic revision of Metarhizium based on a phylogenetic analysis of rRNA sequence data. Mycol. Res", 104, pp.134~150 (2000)
  3. "Production of formulation and application of the ento-mopathogenic fungus Beauveria bassiana for the insect con-trol", pp.3~34 (1994)
  4. "Morphological and genetic characterization of Beauveria spp. from New Zealand. Mycol. Res", 102, pp.250~256 (1998)
  5. "Isolation of DNA from fun-gal mycelia and single spores; in PCR protocols", pp.282~287 (1990), Academic Press
  6. "Biochemistry and physiol- ogy of overwintering in the mature larva of the pine needle gall midge, Thecodiplosis japonensis (Diptera: Cecidomyi- idae) in Korea. Cryo Lett", 21, pp.149~156 (2000)
  7. "Genetic diversity of Beauveria bassiana and relatedness to host insect range. Mycol. Res", 101, pp.159~164 (1997)
  8. "Interkingdom host jumping underground: phylogenetic analysis of entomoparasitic fungi of the genus Cordyceps", 17, pp.629~638 (2000)
  9. "Com- plete nucleotide sequence of Beauveria bassiana 5.8S rRNA coding gene and flanking internal transcribed spacers", 5, pp.381~383 (1995)
  10. "Pathogenicity of Beauveria spp. isolated from Korea against the pine needle gall midge (Thecodiplosis japonensis Uchide et Inouye) and their physiological charac- terization. KFRI. J. For.", 59, pp.57~63 (1998)
  11. "Electron microscopy of modified aluminum alkoxide microstructures on freeze-drying.", 1991, pp.521~524 (1997)
  12. "Microbial control the emergence of anidea", pp.107~26 160 (1956)
  13. "Fungal infection; in InsectPathology", pp.318~387 (1993)
  14. "RAPD-PCR characterization of Beauveria bassiana isolates from the rice water weevil Lissorhoptrus opyzpphillus. Lett. Appl. Microbiol", 25, pp.405~409 (1997)
  15. "Standardized bioassay and screening of Beauveria bassiana and Paecilomyces fumosoroseus against the Russian wheat aphid (Homoptera: Aphididae).", 89, pp.1418~1423 (1996)
  16. "Amplificationand direct sequencing of fungus ribosomal RNAgenes for phylogenetics; in PCR protocols A guide to methodsand applications", pp.315~322 (1990), Academic Press

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