Identification of an Entomopathogenic Fungus, Beauveria bassiana SFB-205 toxic to the Green Peach Aphid, Myzus persicae
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초록
To select entomopathogenic fungi controlling aphidseffectively, several isolates were screened against secondinstars of Myzus persicae nymphs in the glasshouseusing conidia suspension at 1.0×105 conidia/ml. Among these isolates, SFB-205 conidia showed thehighest insecticidal activity about 32.7% efficacy to M. persicae at 4 days after application in the glasshouse. The attachment of SFB-205 conidia on the surface ofM. persicae nymphs, and germination and penetrationwere observed using scanning electron microscopy. SFB-205 was identified as Beauveria bassiana speciesthrough the comparison of 5.8 s rRNA genes. Therewere 24 polymorphisms between SFB-205 and the previouslyreported isolate, B. bassiana ATCC74040 usingsix kinds of primer combinations in amplified fragmentlength polymorphism (AFLP) analysis. The B. bassiana SFB-205 might be used as a practical biologicalcontrol agent for the green peach aphid, M. persicaein the field.
Abstract
To select entomopathogenic fungi controlling aphidseffectively, several isolates were screened against secondinstars of Myzus persicae nymphs in the glasshouseusing conidia suspension at 1.0×105 conidia/ml. Among these isolates, SFB-205 conidia showed thehighest insecticidal activity about 32.7% efficacy to M. persicae at 4 days after application in the glasshouse. The attachment of SFB-205 conidia on the surface ofM. persicae nymphs, and germination and penetrationwere observed using scanning electron microscopy. SFB-205 was identified as Beauveria bassiana speciesthrough the comparison of 5.8 s rRNA genes. Therewere 24 polymorphisms between SFB-205 and the previouslyreported isolate, B. bassiana ATCC74040 usingsix kinds of primer combinations in amplified fragmentlength polymorphism (AFLP) analysis. The B. bassiana SFB-205 might be used as a practical biologicalcontrol agent for the green peach aphid, M. persicaein the field.
참고문헌 (18)
- Ashouri, A., "Pathogenicity of the fungus, Verticillium lecanii, to the green peach aphid, Myzus persicae (Hom.: Aphididae)", Comm. Agri. Appl. Biol. Sci, 69, pp.205~209 (2004)
- Burges, H. D., "Bacteria and fungi as insecticides", Outlook Agric, 11, pp.79~86 (1982)
- Butt, T. M., "Fungi as biocontrol agents: progress, problems and potential" (2001), CABI International, Wallingford
- Charnley, A. K., "Fungal pathogens of insects: cuticle degrading enzymes and toxins", Adv. Bot. Res, 40, pp.241~321 (2003)
- Charnley, A. K., "The role of cuticledegrading enzymes in fungal pathogenesis in insects; in Fungal spore disease initiation in plants and animals", pp.267~287 (1991), Plenum Press, New York, N.Y..
- Clarkson, J. M., "New insights into the mechanisms of fungal pathogenesis in insects", Trends Microbiol, 4, pp.197~203 (1996)
- De Muro, M. A., "The use of amplified fragment length polymorphism for molecular analysis of Beauveria bassiana isolates from Kenya and other countries, and their correlation with host and geographical origin", FEMS Microbiol. Lett, 229, pp.249~257 (2003)
- Devi, K. U., "Genetic diversity, reproductive biology, and speciation in the entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillemin", Genome, 49, pp.495~504 (2006)
- Hajek, A. E., "Interactions between fungal pathogenesis and insect hosts", Annu. Rev. Entomol, 39, pp.293~322 (1994)
- Jansson, R. K., "Influence of nitrogen on population parameters of potato insects: abundance, population growth, and within-plant distribution of the green peach aphid, Myzus persicae (Homoptera: Aphididae)", Environ. Entomol, 15, pp.49~55 (1986), Aphididae).Environ.Entomol.15,49-55.
- Jenkins, N. E., "Development of mass production technology for aerial conidia for use as mycoinsecticides", Biocont. News Inform, 19, pp.21~31 (1998)
- Kassa, A., "Whey for mass production of Beauveria bassiana and Metarhizium anisopliae", Mycol. Res, 112, pp.583~591 (2008)
- Parker, B. L., "Entomopathogenic fungi of Eurygaster integriceps Puton (Hemiptera: Scutelleridae): collection and characterization for development", Biol. Control, 27, pp.260~272 (2002)
- Roberts, D. R., "Entomopathogenic fungi as bioinsecticides; in Frontiers in industrial mycologyAtlas of entomopathogenic fungi" (1992), Springer, Berlin Heidelberg New York
- Shah, P. A., "Entomopathogenic fungi as biocontrol agents", Appl. Microbiol. Biotechnol, 61, pp.413~423 (2003)
- St. Leger, R. J., "Construction of an improved mycoinsecticides overexpressing a toxic protease", Proc. Natl. Acad. Sci., 통권 : 93, pp.6349~6354 (1996)
- Van de Peer, Y., "TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment", Comput. Applic. Biosci, 10, pp.569~570 (1994)
- Vandenberg, J. D., "Standardized bioassay and screening of Beauveria bassiana and Paecilomyces fumosoroseus against the Russian wheat aphid (Homoptera: Aphididiae)", J. Econ. Entomol, 89, pp.1418~1423 (1996)