본문 바로가기
KERD
논문KCI 등재

Study on Selective Media for Isolation of Entomopathogenic Fungi

StuStudy on Selective Media for Isolation of Entomopathogenic Fungidy on Selective Media for Isolation of Entomopathogenic Fungi

1

조회

0

다운로드

3

인용

0

참고문헌

키워드

초록

To determine the optimal isolation conditions of the entomopathogenic fungi from soil, we compared their growth characteristics with non-entomopathogenic fungi on agar media containing various concentrations of cooper (II) chloride (CuCl2) or dodine. The result showed that dodine medium is more selective, and the optimal concentration of dodine is determined with 50 μg/ml. We could isolate several putative entomopathogenic fungi from soil using this, and identify them using ITS analysis. As a result, 64% fungi were identified as typical entomopathogenic fungi. This selective medium may be useful to the rapid and simple isolation of entomopathogenic fungi from soil.

Abstract

To determine the optimal isolation conditions of the entomopathogenic fungi from soil, we compared their growth characteristics with non-entomopathogenic fungi on agar media containing various concentrations of cooper (II) chloride (CuCl2) or dodine. The result showed that dodine medium is more selective, and the optimal concentration of dodine is determined with 50 μg/ml. We could isolate several putative entomopathogenic fungi from soil using this, and identify them using ITS analysis. As a result, 64% fungi were identified as typical entomopathogenic fungi. This selective medium may be useful to the rapid and simple isolation of entomopathogenic fungi from soil.

참고문헌 (27)

  1. Abdo C, "Isolation of Beauveria species from Lebanon and evaluation of its efficacy against the cedar web-spinning sawfly, Cephalcia tannourinensis", Biocontrol, 53, pp.341~352 (2008)
  2. Beilharz VC, "Dodine: A selective agent for certain soil fungi", Trans Br Mycol Soc, 79, pp.507~511 (1982)
  3. Brownbridge M, "Association of entomopathogenic fungi with exotic bark beetles in NewZealand pine plant", Mycopathologia, 169(1), pp.75~80 (2010)
  4. Chase AR, "Selective isolation of the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae from an artificial potting medium", Flo Entol, 69, pp.285~292 (1986)
  5. Da Costa GL, "Pathogenic action of Penicillium species on mosquito vectors of human tropical diseases", J Basic Microbiol, 38(5), pp.337~341 (1998)
  6. Freimoser FM, "Expressed sequence tag (EST) analysis of two subspecies of Metarhizium anisopliae reveals a plethora of secreted proteins with potential activity in insect hosts", Microbiology, 149, pp.239~247 (2003)
  7. Glare TR, "Beauveria caledonica is a naturally occurring pathogen of forest beetles", Mycol Res, 112(3), pp.352~360 (2008)
  8. Hajek AE, "Interactions Between Fungal Pathogens and Insect Hosts", Annu Rev Entomol, 39, pp.293~322 (1994)
  9. Inglis GD, "Use of hyphomycetous fungi for managing insect pests. In: Fungi As Biocontrol Agents: Progress, Problems and Potential", pp.23~69 (2001), CABI Publishing
  10. Keller SP, "Distribution of insect pathogenic soil fungi in Switzerland with special reference to Beauveria brongniartii and Metharhizium anisopliae", Biocontrol, 48, pp.307~319 (2003)
  11. Mark SG, "Fungi: Hyphomycetes. In: Manual of Techniques in Insect Pathology", pp.53~185 (1997), Academic Press
  12. Meyling NV, "Isolation and characterisation of Beauveria bassiana isolates from phylloplanes of hedgerow vegetation", Mycological Research, 110, pp.188~195 (2006)
  13. Meyling NV, "Methods for isolation of entomopathogenic fungi from the soil environment", laboratory manual, pp.1~18 (2007)
  14. Pendland JC, "Evasion of host defense by in vivo-produced protoplast-like cells of the insect mycopathogen Beauveria bassiana", J Bacteriol, 175, pp.5962~5969 (1993)
  15. Santoro PH, "Selection of Beauveria bassiana isolates to control Alphitobius diaperinus", J Invertebr Pathol, 97(2), pp.83~90 (2008)
  16. Shah PA, "Entomopathogenic fungi as biological control agents", Appl Microbiol Biotech, 61, pp.413~423 (2003)
  17. Shimazu M, "Media for selective isolation of an entomogenous fungus, Beauveria bassiana (Deuteromycotina:Hyphomycetes)", Appl Entomol Zool, 31, pp.291~298 (1996)
  18. Shimazu M, "Density of the entomopathogenic fungus, Beauveria bassiana Vuillemin (Deuteromycotina:Hyphomycetes) in forest air and soil", Appl Entomol Zool, 37(1), pp.19~26 (2002)
  19. Tae-Young Shin, "Isolation and Identification of Entomopathogenic Fungus from the Pine Wilt Disease Vector, Monochamus alternatus Hope (Coleoptera: Cerambycidae) in Korea", International Journal of Industrial Entomology, 18(2), pp.125~129 (2009), 한국잠사학회
  20. Srivastava PP, "Utilization of cuticular components of Antheraea mylitta Drury larvae by Penicillium citrinum Thom", Mycopathologia, 128, pp.81~84 (1994)
  21. St. Leger RJ, "Entomopathonic Fungi and the Genomics Era. In: Insect Pathogens: Molecular Approaches and Techniques", pp.365~400 (2009), CABI
  22. Tscharntke T, "Landscape perspectives on agricultural intensification and biodiversity - ecosystem service management", Ecol Lett, 8, pp.857~874 (2005)
  23. Veen KH, "A selective medium for isolation of Beauveria tenella and of Metarrhizium anisopliae", J Invertebr Pathol, 8, pp.268~269 (1966)
  24. Vu VH, "Selection of entomopathogenic fungi for aphid control", J Biosci Bioeng, 104(6), pp.498~505 (2007)
  25. White TJ, "Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications", pp.315~322 (1990), Academic Press Inc
  26. Zimmermann G, "The Galleria bait method for detection of entomopathogenic fungi in soil", Appl Entomol Zool, 102, pp.213~215 (1986)
  27. Zimmermann G, "Suggestion for a standardized method for reisolation of entomopathogenic fungi from soil using the bait method. “Insect pathogens and insect parasitic nematodes”", IOBC Bull, 21(4), pp.289~289 (1998)

관련 논문AI추천

Screening and Evaluation of Antibacterial Metabolites from Entomopathogenic Fungi고승현, 배성민, 신태영, 우수동, 이원우, 최재방한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2013 · 제26권 제2호Isolation and Identification of Entomopathogenic Fungus from the Pine Wilt Disease Vector, Monochamus alternatus Hope (Coleoptera: Cerambycidae) in Korea구현나, 배성민, 신태영, 우수동, Jae-Bang Choi, Jeong-Mi Oh, Ye-Rim Cha한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2009 · 제18권 제2호국내 서남 지역의 산림 토양 특성과곤충병원성 진균과의 상관관계김형찬, 김회리, 이세진한국유기농업학회 · 한국유기농업학회지2023 · 제31권 제1호Comparative Evaluation of Conidia, Blastospores and Culture Filtrates from Entomopathogenic Fungi against Tetranychus urticae곽원석, 김동준, 마지인, 우수동, 윤휘건, 이지훈한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2017 · 제35권 제1호Identification of an Entomopathogenic Fungus, Beauveria bassiana SFB-205 toxic to the Green Peach Aphid, Myzus persicae김재수, 노종열, 제연호, 최재영, Mun Jang Jeon, Sang Chul Shin한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2008 · 제17권 제2호Isolation and Culture of Entomopathogenic Fungus, Cordyceps sphecocephala강석우, 성규병, 홍인표, Chun Ru Li, Mei-Zhen Fan, Sung Hee Nam, Zeng-Zhi Li한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2006 · 제13권 제2호

이 간행물의 다른 논문 (5건)