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cDNA Cloning and mRNA Expression of a Cuticle Protein Gene Homologue from Protaetia brevitarsis

안미영|이은미|김미애|최용수|윤은영|김익수|진병래
한국잠사학회 (Korean Society Of Sericultural Science)|2005.09|International Journal of Industrial Entomology and Biomaterials|제11권 제1호|pp. 67-70

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Abstract

A cuticle protein gene, PbLCP12.1, from the whitespotted flower chafer, Protaetia brevitarsis, was isolated and characterized. The gene contains an ORF of 336 nucleotides capable of encoding a 113 amino acid polypeptide with a predicted molecular mass of 12,138 Da and pI of 4.15. The PbLCP12.1 protein contained a type-specific consensus sequence identifiable in other insect cuticle proteins. The deduced amino acid sequence of the PbLCP12.1 cDNA is most similar to Bombyx mori cuticle protein BmLCP18 (37% protein sequence identity). Northern blot analysis revealed that PbLCP12.1 showed the epidermis-specific expression.

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