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Molecular Cloning and Characterization of a Novel Cuticle Protein Gene from the Chinese Oak Silkmoth, Antheraea pernyi

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Abstract

In our research to identify gene involved in the cuticle protein, we cloned a novel cuticle protein gene,ApCP15.5, from the Chinese oak silkmoth, Antheraea pernyi, larvae cDNA library. The gene encodes a 149 amino acid polypeptide with a predicted molecular mass of 15.5 kDa and a pI of 9.54. The ApCP15.5 contained a type-specific consensus sequence identifiable in other insect cuticle proteins and the deduced amino acid sequence of the ApCP15.5 cDNA is most homologous to Tenebrio molitor-C1B (43% protein sequence identity), followed by Locusta migratoria-76 (42% protein sequence identity). Northern blot and Western blot analyses revealed that the ApCP15.5 showed the epidermis-specific expression. The expression profile of ApCP15.5 indicated that the ApCP15.5 mRNA expression was detected in the early stages after larval ecdysis and larval-pupal metamorphosis, and its expression level was most significant on the first day of larval ecdysis and pupal stage. The ApCP15.5 was expressed as a 15.5 kDa polypeptide in baculovirusinfected insect cells.

참고문헌 (26)

  1. Andersen, "Primary structure of a 14 kDa basic structural protein from the cuticle of the migratory locust", pp.391~76 402 (1993)
  2. Andersen, S. O, "Insectcuticular proteins", 25, pp.153~176 (1995)
  3. Andersen, "Sequencestudies of proteins from larval and pupal cuticle of the yel-low meal worm", pp.121~131 (1997)
  4. Binger, "Identification of thecDNA gene and promoter for a major protein from flexiblecuticles of the giant silkmoth Hyalophora cecropia", pp.989~1000 (1994)
  5. Braquart, C, "Up-regulation of an adult cuticular gene by 20-hydroxyecdysone in insect metamorphosing epidermis culturedin vitro", pp.336~341 (1996)
  6. Charles, "cDNA cloning and deduced amino acidsequence of a major glycine-rich cuticular protein from thecoleopteran Tenebrio molitor Temporal and spatial distributionof the transcript during metamorphosis", pp.813~819 (1992)
  7. Lafont, "Action of different ecdysteroids on the regulation of mRNAsfor the ecdysone receptor and alarval cuticle protein in the larval epidermis of the tobaccohornworm", endocri(l), pp.84~97 (1997)
  8. Hardie, "Hormonalregulation of epidermal metamorphosis in vitro control ofexpression of a larval-specific cuticle gene", pp.369~378 (1991)
  9. Jensen, "Cuticular proteins from the giantcockroach", pp.109~129 (1997)
  10. Kim, "Molecular cloning of three cDNAsencoding putative larval cuticle protein expressed differentiallyafter ecdysis from the mulberry longicorn beetle", pp.803~136 811 (2003)
  11. Kramer, "Down-regulation of expressionof a pupal cuticle protein gene by transcriptional andposttranscriptional control mechanisms during metamorphosisin Galleria", genes e, pp.205~212 (1998)
  12. U, "A factor preventing the major head protein of bacteriophageT4 from random aggregation", pp.69~85 (1970)
  13. Lamp, "Characterization of acDNA and gene encoding a cuticular protein from rigid cuticlesof the giant silkmoth", pp.419~435 (1994)
  14. Locke, "in Microscopic Anatomy of Invertebrates", pp.75~138 (1998)
  15. McMaster, "Analysis of single-and double-stranded nucleic acids on polyacrylamideand agarose gels by using glyoxal and acridine orange", pp.4835~74 4838 (1977)
  16. Izumi, "Structureand developmental expression of a larval cuticle proteingene of the silkworm", tomi, pp.64~74 (1994)
  17. Izumi, "Purification and cDNA cloning of evolutionally conservedlarval cuticle proteins of the silkworm", tomi, pp.701~709 (1997)
  18. Niu, "Structure andspatial expression of the Manduca sexta MSCP14", pp.6 229~240 (1997)
  19. A, "Developmental expression of three genes for larvalcuticular proteins of the tobacco hornworm", pp.82~94 (1986)
  20. I, "organization and expression of two clustered cuticleprotein genes during the metamorphosis of an insect", pp.304~312 (1998)
  21. Sawada, "Molecular cloning and characterization of acDNA encoding a novel cuticle protein in the silkworm", pp.519~134 527 (2003)
  22. Comp, "Molecular cloning and characterization of a cDNAencoding a larval cuticle protein of Bombyx mori", tomi(and s), pp.105~122 109 (1999)
  23. Snyder, "Cuticle protein genes of Drosophila organization and evolution of four clusteredgenes", pp.1027~1040 (1982)
  24. Izumi, "Structural analysis of gene encodingcuticle protein BMCP18 and characterization of its putativetranscription factor in the silkworm", tomi, pp.611~620 (2001)
  25. Gordon, "Electrophoretictransfer of proteins from polyacrylamide gels to nitrocellulosesheets", pp.4350~76 4354 (1979)
  26. Vaughn, "The establishment of two insect cell lines fromthe insect Spodoptera frugiperda", (ctuidae), pp.213~217 (1977)

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