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A LIM Protein Gene Homologue of Protaetia brevitarsis: cDNA Cloning and mRNA Expression

이은미|김미애|최용수|김성렬|김익수|황재삼|진병래
한국잠사학회 (Korean Society Of Sericultural Science)|2005.09|International Journal of Industrial Entomology and Biomaterials|제11권 제1호|pp. 71-74

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Abstract

A LIM protein gene homologue of the CRP (cysteinerich protein) family in the whiter-spotted flower chafer,Protaetia brevitarsis, was cloned. The P. brevitarsis LIM protein cDNA encodes a 92 amino acid polypeptide with a predicted molecular mass of 10,030 Da and a pI of 8.57. The P. brevitarsis LIM protein contains the cysteine-rich consensus sequence of LIM domain and the glycine-rich consensus sequence observed in the cysteine-rich protein family 1 (CRP1). The potential nuclear targeting signal is retained. The deduced amino acid sequence of the P. brevitarsis LIM protein cDNA showed 92% identity to another beetle, Apriona germari LIM protein. Northern blot analysis showed that P. brevitarsis LIM protein is highly expressed in epidermis and midgut, but not in the fat body.

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