논문KCI 등재
A Chymotrypsin Gene Homologue from the Mulberry Longicorn Beetle, Apriona germari: cDNA Sequence Characterization and mRNA Expression Pattern
2
조회
0
다운로드
0
인용
0
참고문헌
초록
Abstract
A chymotrpsin gene homologue was cloned from the mulberry longicorn beetle, Apriona germari. The A. germari chymotrypsin cDNA contains an ORF of 950 nucleotides capable of encoding a 283 amino acid polypeptide with a predicted molecular mass of 29151 Da and pI of 9.38. The A. germari chymotrypsin has conserved six cysteine residues and active triad formed by His, Asp and Ser. The deduced amino acid sequence of the A. germari chymotrypsin cDNA was closest in structure to the Anthonomus grandis chymotrypsin.Northern blot analysis revealed that A. germari chymotrypsin showed the midgut-specific expression.
참고문헌 (14)
- "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.", 25, pp.3389~3402 (1997)
- "A gene family in Drosophila melanogastercoding for trypsin-like enzymes. Nucl. Acids Res. 13", pp.6605~6619 (1985)
- "Serine protease mechanism and specificity", 102, pp.4501~4523 (2002)
- "Molecular cloning of a cDNA encoding a cathepsin D homologue from the mulberry longicorn beetle, Apriona germari.", 3, pp.121~126 (2001)
- "Molecular cloning of three cDNAs encoding putative larval cuticle protein expressed differentially after larval ecdysis from the mulberry longicorn beetle", B 136, pp.803~811 (2003)
- "Identification of six chymotrypsin cDNAs from larval midguts of Helicoverpa zea and Agrotis ipsilon feeding on the soybean (Kunitz)", 31, pp.633~644 (2001)
- "Analysis of single-and double-stranded nucleic acids on polyacrylamideand agarose gels by using glyoxal and acridine orange. Proc.Natl. Acad. Sci. USA 74", pp.4835~4838 (1977)
- "Early trypsin, a female-specific midgut protease in Aedes aegypti: isolation, aminoterminal sequence determination", pp.119~126 (1996)
- "A diverse family of serine proteinase expressed in cotton boll weevil (Anthonomus grandis): implications for the design of pest-resistant transgenic cotton plants", 34(mus), pp.903~918 (2004)
- "Cloning and characterization of trypsin- and chymotrypsin-like proteases from the midgut of the sand fly vector Phlebotomus papatasi.", 33, pp.163~171 (2003)
- "Serine proteinase of the human body louse (Pediculus humanus): sequence characterization and expression patterns", 97, pp.486~500 (2005)
- "Molecular characterization of two serine proteases expressed in gut tissue of the African trypanosome vector, Glossina morsitans morsitans.", 10, pp.47~56 (2001)
- "Life cycle of the mulberrylongicorn beetle Apriona germari Hope on an artificial diet", 2, pp.169~173 (1999)
- "Molecular cloning of trypsin-like cDNAs and comparison of proteinase activities in the salivary glands and gut of the tarnished plant bug Lygus lineolaris (Heteroptera: Miridae)", 33, pp.889~899 (2003)
관련 논문AI추천
Molecular Cloning of a Cdna Encoding a Cathepsin B Homologue from the Mulberry Longicorn Beetle,Apriona germari윤형주, 진병래, HungDaeSohn, JaeYuMoon, NamSookPark, SangMongLee, SeongRyulKim한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2002 · 제4권 제1호Cloning and mRNA Expression of an Actin cDNA from the Mulberry Longicorn Beetle, Apriona germari김익수, 손흥대, 윤형주, 이광식, 진병래, Xijie Guo, Yadong Wei, Zhongzheng Gui한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2004 · 제9권 제2호Molecular Cloning of a LIM Protein cDNA from the Mulberry Longicorn Beetle, Apriona germari김익수, 손흥대, 윤형주, 진병래, Xijie Guo, Yadong Wei, Zhongzheng Gui한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2004 · 제9권 제1호Apriona germari Larval Cuticle Protein Genes: Genomic Structure of Three Cuticle Protein Genes and cDNA Cloning of a Novel Cuticle Protein손흥대, 윤형주, 진병래, Bo Yeon Kim, Gui Zhong Zheng, Guo Xijie, Ya Dong Wei한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2007 · 제14권 제1호cDNA Cloning and mRNA Expression of a Cuticle Protein Gene Homologue from Protaetia brevitarsis김미애, 김익수, 안미영, 윤은영, 이은미, 진병래, 최용수한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2005 · 제11권 제1호Cdna Sequence and mRNA Expression of a Novel Serine Protease from the Firefly,Pyrocoelia rufa손흥대, 이광식, 진병래, SeongRyulKim한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2002 · 제5권 제1호
이 간행물의 다른 논문 (10건)
Mode of Transmission of A New Microsporidian and Its Affect on Fecundity and Hatching in Silkmoth, Bombyx mori L.B. Nataraju, Shabir Ahmad Bhatpp. 81-86Isolation and Characterization of Plutella xylostella Granulovirus Isolated in Korea왕용, Ming Shun Li, 강중남, 노종열, 진병래, 제연호, 최희규, 최재영, 김양수, 임대준pp. 87-91Effect of Extended Egg Preservation Schedule in Conservation of Mutant Silkworm (Bombyx mori L.) Genetic Stocks in Gene BankK. Thangavelu, M. Muthulakshmi, B. Mohan, N. Balachandran, R. K. Sinhapp. 93-98Oviposition and Colony Development of the Bumblebees, Bombus ignitus and B. terrestris depending on Different Pollen이경용, 이상범, 김삼은, 윤형주, 박인균pp. 99-105Seasonal Impact of Microsporidian Infection on the Reproductive Potential of Silkworm, Bombyx mori L. (Lepidoptera: Bombycidae)B. Saratchandra, N. Krishnan, N. K. Das, N. Madana Mohanan, D. P. Haldar, P. Mitrapp. 107-111
