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cDNA Cloning and Developmental Expression of Hemolin in Bombyx mandarina

김경아|김남순|이진성|강석우|노시갑|강민욱
한국잠사학회 (Korean Society Of Sericultural Science)|2005.06|International Journal of Industrial Entomology and Biomaterials|제10권 제2호|pp. 101-106

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Abstract

In this study, we describe the Bombyx mandarina hemolin cDNA. A sequence analysis of cDNA revealed a single open reading frame (ORF) of 1233 nucleotides. The deduced 410 amino acid sequence of B. mandarina hemolin contains 4 imunoglobulin (Ig) C-2 type domains. B. mandarina hemolin cDNA showed the highest sequence homology to known those of B. mori.The developmental profile in terms of expression level of hemolin mRNA was determined in the absence of a bacterial challenge. Hemolin mRNA was detected only in mid-gut, but not in hemocytes, fat body, testis, and silkglands. Hemolin mRNA in mid-gut was not detected until the spinning stage of the last instar larva, however,it dramatically increased at the beginning of spinning and gradually decreased until pupal stage.

참고문헌 (24)

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