Operation Modes Can Affect the Activity of Immobilized Enzyme onto Silk Fibroin Nanofibrous Membrane
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In the present study, we report that the selection of operation mode is important to take thefull advantage of nanofibrous membrane in enzyme immobilization. Silk fibroin nanofibrousmembrane has been prepared by electrospinning, and a-chymotrypsin was immobilized asa model enzyme. When the immobilized enzyme was operated in the membrane reactormode, the Michaelis constant, Km, was lower and the Vmax was higher compared to the batchreactor mode. No concentration gradient was observed in the membrane reactor mode andthe immobilized enzyme was stable even after 7 times of re-use. Our results suggests that theenzyme immobilized nanofibrous membrane should be operated in the membrane reactormode rather than in the bath reactor mode
Abstract
In the present study, we report that the selection of operation mode is important to take thefull advantage of nanofibrous membrane in enzyme immobilization. Silk fibroin nanofibrousmembrane has been prepared by electrospinning, and a-chymotrypsin was immobilized asa model enzyme. When the immobilized enzyme was operated in the membrane reactormode, the Michaelis constant, Km, was lower and the Vmax was higher compared to the batchreactor mode. No concentration gradient was observed in the membrane reactor mode andthe immobilized enzyme was stable even after 7 times of re-use. Our results suggests that theenzyme immobilized nanofibrous membrane should be operated in the membrane reactormode rather than in the bath reactor mode
참고문헌 (6)
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