Activity and Stability of Immobilized Enzyme on Silk Sericin Bead
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In present preliminary report, we showed the possibility of silk sericin (SS) in enzymeimmobilization. SS beads were prepared and enzymes were immobilized on it. The specificactivity of immobilized a-chymotrypsin retained more than 87% compared to the freeenzyme. The immobilized a-chymotrypsin has better stability against ethanol especially thoseimmobilized on SS beads coagulated in methanol. Immobilized trypsin and lipase had alsocomparable apparent activity compared to free enzyme. Our result indicates that SS could bea good candidate for enzyme immobilization support due to its hydrophilicity.
Abstract
In present preliminary report, we showed the possibility of silk sericin (SS) in enzymeimmobilization. SS beads were prepared and enzymes were immobilized on it. The specificactivity of immobilized a-chymotrypsin retained more than 87% compared to the freeenzyme. The immobilized a-chymotrypsin has better stability against ethanol especially thoseimmobilized on SS beads coagulated in methanol. Immobilized trypsin and lipase had alsocomparable apparent activity compared to free enzyme. Our result indicates that SS could bea good candidate for enzyme immobilization support due to its hydrophilicity.
참고문헌 (11)
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