Preparation and Characterization of Silk Fibroin Nanoparticles
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초록
Silk fibroin was conjugated with methoxypoly(ethylene glycol) derivatives to prepare silk nanoparicles. Conjugation of SF with PEG was examined with various instrumental analyses. Nuclear magnetic resonance spectrometry and amino acid analysis showed that serine and tyrosine residues in SF were reacted with PEG and resulted in increasing molecular weight. The sizes and shapes of SF nanoparticles observed by transmission electronmicroscope were ranged about 150-400 nm in diameter and spherical morphology. UV/VIS spectrometry showed SF nanoparticles might be outer PEG and inner SF structure.
Abstract
Silk fibroin was conjugated with methoxypoly(ethylene glycol) derivatives to prepare silk nanoparicles. Conjugation of SF with PEG was examined with various instrumental analyses. Nuclear magnetic resonance spectrometry and amino acid analysis showed that serine and tyrosine residues in SF were reacted with PEG and resulted in increasing molecular weight. The sizes and shapes of SF nanoparticles observed by transmission electronmicroscope were ranged about 150-400 nm in diameter and spherical morphology. UV/VIS spectrometry showed SF nanoparticles might be outer PEG and inner SF structure.
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