Effect of Silk Fibroin Hydrolysate on the Apoptosis of MCF-7 human Breast Cancer Cells
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Breast cancer is one of the most common cancers among women worldwide. Recentlyanticancer agents have been developed using natural substances. To evaluate the anticancereffect of hydrolysates of silk fibroin (HSF), we investigated the effect of HSF on cell viability andapoptosis of a breast cancer cell line, MCF-7, induced through the mitochondrial pathway. Theresult showed that HSF decreased cell viability in MCF-7 cells in a dose- and time-dependentmanner, resulting in an increase in the sub-G1 phase cell population. HSF increased the levelof the pro-apoptotic Bax protein and decreased the levels of the anti-apoptotic Bcl-2 protein. Inaddition, HSF induced apoptosis in MCF-7 cells through a mitochondria-dependent pathwayby increasing levels of cytochtome c, and cleavage of PARP. Taken together, these findingssuggest that HSF inhibits the proliferation of MCF-7 breast cancer cells through a mitochondriaand caspase dependent apoptotic pathway.
Abstract
Breast cancer is one of the most common cancers among women worldwide. Recentlyanticancer agents have been developed using natural substances. To evaluate the anticancereffect of hydrolysates of silk fibroin (HSF), we investigated the effect of HSF on cell viability andapoptosis of a breast cancer cell line, MCF-7, induced through the mitochondrial pathway. Theresult showed that HSF decreased cell viability in MCF-7 cells in a dose- and time-dependentmanner, resulting in an increase in the sub-G1 phase cell population. HSF increased the levelof the pro-apoptotic Bax protein and decreased the levels of the anti-apoptotic Bcl-2 protein. Inaddition, HSF induced apoptosis in MCF-7 cells through a mitochondria-dependent pathwayby increasing levels of cytochtome c, and cleavage of PARP. Taken together, these findingssuggest that HSF inhibits the proliferation of MCF-7 breast cancer cells through a mitochondriaand caspase dependent apoptotic pathway.
참고문헌 (38)
- Acharya C, "Silk fibroin protein from mulberry and non-mulberry silkworms: Cytotoxicity, biocompatibility and kinetics of L929 murine fibroblast adhesion", J Mater Sci Mater Med, 19, pp.2827~2836 (2008)
- Anelli TFM, "Tamoxifen administration is associated with a high rate of treatmentlimiting symptoms in male breast cancer patients", Cancer, 74, pp.74~77 (1994)
- Chien S, "Quercetin-induced apoptosis acts through mitochondrialand caspase-3-dependent pathways in human breast cancer MDAMB-231 cells", Human Toxicol, 28, pp.493~503 (2009)
- Cotter TG, "Apoptosis and cancer: The genesis of a research field", Nat Rev Cancer, 9, pp.501~507 (2009)
- Danial NN, "Cell death: Critical control points", Cell, 116, pp.205~219 (2004)
- de Bruin EC, "Apoptosis and non-apoptotic deaths in cancer development and treatment response", Cancer Treat Rev, 34, pp.737~749 (2008)
- Desagher S, "Mitochondria as the central control point of apoptosis", Trends Cell Biol, 10, pp.369~377 (2000)
- Donovan M, "Control of mitochondrial integrity by bcl-2 family members and caspase-independent cell death", BBA, 1644, pp.133~147 (2004)
- Evan GI, "Proliferation, cell cycle and apoptosis in cancer", Nature, 411, pp.342~348 (2001)
- Ghobrial IM, "Targeting apoptosis pathways in cancer therapy", CA Cancer J Clin, 55, pp.178~194 (2005)
- Hanawa T, "New oral dosage form for elderly patients. II. Release behavior of benfotiamine from silk fibroin gel", Chem Pharm Bull, 43, pp.872~876 (1995)
- Hajra KM, "Apoptosome dysfunction in human cancer", Apoptosis, 9, pp.691~704 (2004)
- Hengartner MO, "The biochemistry of apoptosis", Nature, 407, pp.770~776 (2000)
- Herceg Z, "Functions of poly(ADP-ribose)polymerase(PARP)in DNA repair, genomic integrity and cell death", Mutat Res-Fund Mol M, 477, pp.97~110 (2001)
- Igney FH, "Death and anti-death: Tumor resistance to apoptosis", Nat Rev Cancer, 2, pp.277~288 (2002)
- Jin S, "Daidzein induces MCF-7 breast cancer cell apoptosis via the mitochondrial pathway", Ann Oncol, 21, pp.263~268 (2010)
- Kallio A, "Role of mitochondria in tamoxifen-induced rapid death of MCF-7 breast cancer cells", Apoptosis, 10, pp.1395~1141 (2005)
- Kato N, "Silk protein, sericin, inhibits lipid peroxidation and tyrosinase activity", Biosci Biotechnol Biochem, 62, pp.145~147 (1998)
- Kim ED, "Fibroin-derived peptides stimulate glucose transport in normal and insulin-resistant 3T3-L1 adipocytes", Biol Pharm Bull, 32, pp.427~433 (2009)
- Kluck RM, "The release of cytochrome c from mitochondria: A primary site for bcl-2 regulation of apoptosis", Science, 275, pp.1132~1136 (1997)
- Kroemer G, "Mitochondrial membrane permeabilization in cell death", Physiol Rev, 87, pp.99~163 (2007)
- Kwang Gill Lee, "Cocoon Filament Quality of a Special Silkworm Variety, GoldenSilk", International Journal of Industrial Entomology, 24(1), pp.17~21 (2012), 한국잠사학회
- Minoura N, "Attachment and growth of cultured fibroblast cells on silk protein matrices", J Biomed Mater Res 29, 29, pp.1215~1221 (1995)
- Mousavi SH, "Role of caspases and bax protein in saffron-induced apoptosis in MCF-7 cells", Food Chem Toxicol, 47, pp.1909~1913 (2009)
- 남미경, "적양배추 추출물이 인체 유방암 세포 MDA-MB-231의 세포사멸에 미치는 영향", 한국식품영양과학회지, 42(1), pp.8~16 (2013), 한국식품영양과학회
- Nishida A, "Use of silk protein, sericin, as a sustained-release material in the form of a gel, sponge and film", Chem Pharm Bull, 58, pp.1480~1486 (2010)
- Roh DH, "Wound healing effect of silk fibroin", alginate-blended sponge in full thickness skin defect of rat, 2006, pp.17, 547~552
- Hye-Young Shim, "Acacetin-induced Apoptosis of Human Breast Cancer MCF-7 Cells Involves Caspase Cascade, Mitochondria-mediated Death Signaling and SAPK", JNK1, Molecules and Cells, pp.2007, 24(1)[DOI]
- Sofia S, "Functionalized silk-based biomaterials for bone formation", J Biomed Mater Res, 54, pp.139~148 (2001)
- Solary E, "The role of apoptosis in the pathogenesis and treatment of diseases", Eur Respir J, 9, pp.1293~1305 (1996)
- Taraphdar AK, "Natural products as inducers of apoptosis: Implication for cancer therapy and prevention", Curr Sci India, 80, pp.1387~1396 (2001)
- Tsukada M, "Preparation and application of porous silk fibroin materials", J Appl Polym Sci, 54, pp.507~514 (1994)
- Wang X, "Anticancer activity of litchi fruit pericarp extract against human breast cancer in vitro and in vivo", Toxicol Appl Pharmacol, 215, pp.168~178 (2006)
- Wang G, "The use of silk fibroin", hydroxyapatite composite co-cultured with rabbit bonemarrow stromal cells in the healing of a segmental bone defect, 2010, pp.92, 320~325
- Zhang G, "Early detection of apoptosis using a fluorescent conjugate of annexin V", Biotechniques, 23, pp.525~531 (1997)
- Zhou C, "Silk fibroin: Structural implications of a remarkable amino acid sequence", Proteins, 44, pp.119~122 (2001)
- Zhu W, "Hypoxia and serum deprivation-induced apoptosis in mesenchymal stem cells", Stem Cells, 24, pp.416~425 (2006)
- Zimmermann KC, "The machinery of programmed cell death", Pharmacol Ther, 92, pp.57~70 (2001)
