토사자 추출물이 MCF-7 유방암 세포의 세포자멸사에 미치는 영향
Effects of Cuscutae Semen Water Extract on Apoptosis of MCF-7 Human Breast Cancer Cells
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Objectives: This study aimed to evaluate the effects of Cuscutae Semen waterextract (CS) on MCF-7 human breast cancer cells. Methods: To clarify the results, we cultivated MCF-7 cells in cell culture plates. And then we extracted each of 100 μg/ml, 300 μg/ml, 600 μg/ml CS, gave it toMCF-7 cell. After these process we performed MTT assay to elucidate the abilityof apoptosis. The result of mRNA was analyzed by RT-PCR. Results: Each of concentrated extracts CS decreased the survival rate of MCF-7cells. CS decreased Bcl-2 which is known as a blocking cell apoptosis. Bax, caspase-3,P21 and RIP-1 that accelerate apoptogenic activity factors increased by CS. CSdid not change the condition of caspase-8, caspase-9, P53 factors on MCF-7 cells. Furthermore caspase-8, caspase-9, P53 factors on MCF-7 cells does not make itmore active but turn it on. Conclusions: According to the above results, we could suggest that CS can occurthe apoptosis on MCF-7 cells.
Abstract
Objectives: This study aimed to evaluate the effects of Cuscutae Semen waterextract (CS) on MCF-7 human breast cancer cells. Methods: To clarify the results, we cultivated MCF-7 cells in cell culture plates. And then we extracted each of 100 μg/ml, 300 μg/ml, 600 μg/ml CS, gave it toMCF-7 cell. After these process we performed MTT assay to elucidate the abilityof apoptosis. The result of mRNA was analyzed by RT-PCR. Results: Each of concentrated extracts CS decreased the survival rate of MCF-7cells. CS decreased Bcl-2 which is known as a blocking cell apoptosis. Bax, caspase-3,P21 and RIP-1 that accelerate apoptogenic activity factors increased by CS. CSdid not change the condition of caspase-8, caspase-9, P53 factors on MCF-7 cells. Furthermore caspase-8, caspase-9, P53 factors on MCF-7 cells does not make itmore active but turn it on. Conclusions: According to the above results, we could suggest that CS can occurthe apoptosis on MCF-7 cells.
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