五靈脂가 유방암세포의 사멸에 미치는 영향
Effects of Trogopterorum Faeces on the Apoptostic Cell Death in Breast Cancer Cells
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Objectives: This study was designed to investigate the effects of TrogopterorumFaeces on the apoptostic cell death in breast cancer cells. Methods: In the experiment, the effects of Trogopterorum Faeces on proliferationrates and type of cell death were investigated using MCF-7 cells in vitro. The effectson expression levels of caspase 3 and caspase 9 were also investigated. Results: The effects on expression levels of caspase 3 and caspase 9 were alsoinvestigated. In the present results, treatment with Trogopterorum Faeces decreasedproliferation rates in a dose dependent manner. ID50 (50 % inhibitory dosage) was177.2 μg/ml. In addition, treatment with Trogopterorum Faeces increased percentageof apoptotic cells. Finally the expression level of caspase 3 and caspase 9 wereelevated by treatment with Trogopterorum Faeces respectively. Conclusions: This study suggests that Trogopterorum Faeces can trigger caspasedependent apoptosis in MCF-7 cells.
Abstract
Objectives: This study was designed to investigate the effects of TrogopterorumFaeces on the apoptostic cell death in breast cancer cells. Methods: In the experiment, the effects of Trogopterorum Faeces on proliferationrates and type of cell death were investigated using MCF-7 cells in vitro. The effectson expression levels of caspase 3 and caspase 9 were also investigated. Results: The effects on expression levels of caspase 3 and caspase 9 were alsoinvestigated. In the present results, treatment with Trogopterorum Faeces decreasedproliferation rates in a dose dependent manner. ID50 (50 % inhibitory dosage) was177.2 μg/ml. In addition, treatment with Trogopterorum Faeces increased percentageof apoptotic cells. Finally the expression level of caspase 3 and caspase 9 wereelevated by treatment with Trogopterorum Faeces respectively. Conclusions: This study suggests that Trogopterorum Faeces can trigger caspasedependent apoptosis in MCF-7 cells.
참고문헌 (40)
- Ferlay J, "Cancer incidence and mortality patterns in Europe: estimates for 40 countries in 2012", Eur J Cancer, 49, pp.1374~1403 (2013)
- "Breast cancer statistics" (2013)[URL]
- Shim JK, "A study on dose analysis of lung and cardiac with respiration methods in radiotherapy of breast cancer", pp.2010
- Editing commission of Globooks, "Gynecology" (2009), Globooks
- Kim JJ, "A bibliographic study on the treatment of the breast mass", The Journal of Korean Obstetrics & Gynecology, 12(4), pp.29~43 (1998)
- 황은경, "항호르몬요법을 받는 유방암 환자의 삶의 질 영향 요인", Journal of Korean Academy of Nursing, 44(1), pp.108~117 (2014), 한국간호과학회
- 정재원, "Details of Lymphedema, Upper Limb Morbidity, and Self Management in Women after Breast Cancer Treatment", 여성건강간호학회지, 17(5), pp.474~483 (2011), 여성건강간호학회
- The compilation committee of Herbalogy, University College of Korean Medicine, "Herbalogy textbook of The national University College of Korean Medicine", pp.464~465 (2005), Yeongrimsa
- Moon CM, "Inhibiting effect of faeces trogopterori on MCP-1 gene expression", pp.2005
- 김병진, "오령지 추출물의 염증성 세포활성물질 억제효과", 대한본초학회지, 24(3), pp.153~160 (2009), 대한본초학회
- Oh YE, "Effect of trogopterus xanthipes milen-edwards and paeonia lactiflora pallas extracts on uterine leiomyoma cell", pp.2012
- Lee YW, "Study on inhibition of metastasis of acute skin infection via method of promoting blood flow to eliminate blood stasis", Daejeon University, Institute of korean medicine, Thesis Collection, 7(2), pp.437~451 (1999)
- Jeong G, "An experimental study on the effect to the serum prothrobin time in mouse by the pteropi stercus and tythae pollen extract administration", The Kyung Hee University Oriental Medical Journal, 2(1), pp.171~181 (1979)
- Oh YS, "Apoptotic effect of Sojucktang on human KLE endometrial cancer cells", pp.2008
- Lee YS, "Clinical case report on the treatment of medicines for promoting blood circulation to remove blood stasis(沽血化瘀劑) on Three Patients With an Ovarian Cyst", The Journal of Korean Obstetrics & Gynecology, 14(3), pp.218~227 (2001)
- 박영선, "자궁근종 2례 임상경과 보고", 대한한방부인과학회지, 17(3), pp.199~208 (2004), 대한한방부인과학회
- Shin SW, "Study on apoptosisinducing mechanism of Sujeom-san", The Korea Journal of Physiology &Pathology, 15(5), pp.745~756 (2001)
- Shin WW, "Study on antitumor activity of Sobokchukeotang and Kamisobokchukeotang", The Journal of Korean Oriental Medicine, 22(2), pp.22~30 (2001)
- The Society of Korean Obstetrics and Gynecology, "Korean Obstetrics and Gynecology(2)", pp.371~390 (2012), Euiseongdang
- Jone DB, "Cancer in the contralateral breast after radiotherapy for breast cancer", The New England Journal of Medicine, 326, pp.781~785 (1992)
- Son SM, "Predictive factors of fatigue in breast cancer patients undergoing chemotherapy", pp.2009
- Moxley G, "Rheumatic disorders and functional disability with aromatase inhibitor therapy", Clinical Breast Cancer, 10(2), pp.144~147 (2010)
- Huh J, "Dong-Eui-Bo-Kham", pp.683~ (2006), Dong-Eui-Bo-Kham
- 용형순, "황금의 유방암세포주에 대한 항암작용", 대한한방내과학회지, 25(3), pp.451~460 (2004), 대한한방내과학회
- 양동선, "울금이 MDA-MB-231 세포 및 DMBA로 유발된 흰쥐의 유방암에 미치는 영향", 대한한방부인과학회지, 26(3), pp.44~58 (2013), 대한한방부인과학회
- 정경아, "三稜 추출물의 인간 유방암 세포 성장 억제 효과", 대한한방부인과학회지, 19(1), pp.166~177 (2006), 대한한방부인과학회
- Song KC, "The clinical study in 60 cases for breast cancer patients on the effects by Hangamdan(抗癌丹)", The Journal of Korean Oriental Internal Medicine, 22(4), pp.669~674 (2001)
- 반혜란, "歸朮破?湯 추출물의 인간 유방암세포에 대한 성장억제 효과", 대한한방부인과학회지, 19(1), pp.155~165 (2006), 대한한방부인과학회
- Hong WS, "Whang Jae Nae Kyung", pp.103~104 (1974), Komoonsa
- Lee SH, "A bibliographic study on the inhibitory of tumor growth, and metastasic using Hwalhyulhwau", Daejeon University, Institute of korean medicine, Thesis Collection, 7(1), pp.700~ (1998)
- Meers P, "Phospholipid determinants for annexin V binding sites and the role of tryptophan", Biochemistry, 33(19), pp.5829~5837 (1994)
- Koopman G, "Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis", Blood, 84(5), pp.1415~1420 (1994)
- Vermes I, "A novel assay for apoptosis-flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V", Journal of Immunology Methods, 184(1), pp.39~51 (1995)
- Lecoeur H, "Nuclear apoptosis detection by flow cytometry: influence of endogenous endonucleases", Exp. Cell Res, 277(1), pp.1~14 (2002)
- Alnemri ES, "Human ICE", CED-3 Protease Nomenclature, 1996, pp.87(2), 171~
- Chu ZL, "A novel enhancer of the apaf1apoptosome involved in cytochrome c-dependent caspase activation and apoptosis", J. Biol. Chem, 276(12), pp.9239~9245 (2001)
- Alnemri ES, "Human ICE", CED-3protease nomenclature, 1996, pp.87(2), 171~
- S. Fulda, "K-M. Debatin extrinsic versus intrinsic apoptosis pathways in anticancer chemotherapy", Oncogene, 25(34), pp.4798~4811 (2006)
- Salvesen GS, "Caspases : opening the boxes and interpreting the arrows", Cell Death Differ, 9(1), pp.3~5 (2002)
- Ghavami S, "Apoptosis and cancer:mutations within caspase genes", J. Med. Genet, 46(8), pp.497~510 (2009)
