난소절제술한 백서에 식립한 임플란트에 대한 PostGraft™의 경구 투여 효과
The effect of PostGraft™ on implants were installed at the tibia of ovariectomized rats
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연구 목적: 본 연구에서는 난소절제술을 시행한 백서의 골결손부에 골이식을 시행하고 소형 임플란트를 제작하여 식립한 후 PostGraft™를 투여하여 임플란트의 골유착 및 안정성에 미치는 영향에 대해 알아보고자 하였다. 연구 재료 및 방법: 난소절제술을 시행한 백서 48 마리의 골결손부에 Biocera™를 사용하여 골이식을 시행하고 소형 임플란트를 제작하여 식립한 후 PostGraft™를 투여한 군 24마리와 투여하지 않은 대조군 24마리를 식립 2, 4, 6주 후 Periotest®를 이용한 임플란트 안정성, 골밀도, 골-임플란트 접촉률 측정 및 조직학적 검사 등을 통하여 비교 분석하였다. 결과: Periotest®측정 결과 시간이 지남에 따라 실험군과 대조군에서 모두 측정값이 감소하였고 실험군에서 더 낮은 값을 나타내었으나 각 군 간에 유의한 차이는 관찰되지 않았다. 골밀도 값은 골다공증으로 인해, 시간이 경과함에 따라 실험군과 대조군에서 모두 감소하였고 실험군에서 더 적은 감소량을 보였으나 각 군 간에 유의한 차이는 관찰되지 않았다. 골-임플란트 접촉률 측정 결과 시간이 경과함에 따라 실험군과 대조군 모두 증가하였고 실험군에서 더 높은 값을 보였으나 2주와 4주에서는 각 군 간에 유의한 차이가 없었고 6주에서는 각 군 간에 유의한 차이가 관찰되었다(P<.05). 조직학적 검사 결과 시간이 경과함에 따라 실험군과 대조군 모두 골반응이 증가하였으며 실험군에서 더 활발한 골형성이 이루어졌다. 결론: PostGraft™의 경구 투여가 난소절제술을 시행한 백서의 경골에 식립한 임플란트의 골유착과 골형성을 향상시키는 효과가 있을 수 있다.
Abstract
Purpose: This study evaluated PostGraft™ which enhances implant stability and bone density. Materials and methods: Forty eight implants were installed at the tibia of ovariectomized rats. The group administrated with PostGraft™ was the experimental group, and the control group was not administrated. Implant stability was evaluated at the 2nd, 4th and 6th week by Periotest value, bone mineral density, bone-to-implant contact. These values were analyzed statistically with Mann-Whitney U test (P<.05). Histological analysis was evaluated at the 2nd, 4th and 6th week. Results: According to the Periotest®. measurement, both experimental and control groups showed decrease in values as time elapsed. Greater decrease was observed in the experimental group but there was no significant difference. By examining the radiographic images, both experimental and control groups showed tendency of increase in bone density. Greater increase was seen in the experimental group but there was no significant difference. According to the bone-to-implant contact measurement, both experimental and control groups showed increase in values as time elapsed. Greater increase was seen in the experimental group. At the 2nd and 4th week, there was no significant difference. But at the 6th week, there was significant difference (P<.05). By histological analysis, both experimental and control groups showed ncrease in bone formation as time elapsed. In addition, greater increase was seen in the experimental group. Conclusion: It could be concluded that the PostGraft™ medicated group showed better results in the bone density and osseointegration.
참고문헌 (19)
- Sykaras N, "Implant materials, designs, and surface topographies: their effect on osseointegration. A literature review", Int J Oral Maxillofac Implants, 15, pp.675~690 (2000)
- Le Gue′hennec L, "Surface treatments of titanium dental implants for rapid osseointegration", Dent Mater, 23, pp.844~854 (2007)
- Cochran DL, "A comparison of endosseous dental implant surfaces", J Periodontol, 70, pp.1523~1539 (1999)
- Albrektsson T, "Osseointegrated titanium implants. Requirements for ensuring a long-lasting, direct bone-to-implant anchorage in man", Acta Orthop Scand, 52, pp.155~170 (1981)
- Kim JK, "Effect of Rehmannia glutinosa Libosch and Acanthopanax senticosus extracts on osteoblastic activity and proliferation and osteoclastic bone resorption", Korean J Bone Metab, 9, pp.133~143 (2002)
- Zhang RX, "Rehmannia glutinosa: review of botany, chemistry and pharmacology", J Ethnopharmacol, 117, pp.199~214 (2008)
- Amano S, "Stimulatory effect on bone resorption of interleukin-1-like cytokine produced by an osteoblast-rich population of mouse calvarial cells", Calcif Tissue Int, 43, pp.88~91 (1988)
- Ritchlin CT, "Mechanisms of TNF-alpha- and RANKL-mediated osteoclastogenesis and bone resorption in psoriatic arthritis", J Clin Invest, 111, pp.821~831 (2003)
- Park SW, "The effect of PRF on the osseointegration and stability of implant", 석사, pp.2005
- 강성진, "OPB-K가 임플랜트의 골유착 및 안정성에 미치는 영향에 관한 연구", 대한치과보철학회지, 46(1), pp.31~41 (2008), 대한치과보철학회
- 최유성, "임플란트 식립 환자의 PostPlant(TM) Calcium 투여 효과에 대한 임상연구", 대한치과보철학회지, 48(02), pp.128~134 (2010), 대한치과보철학회
- Anderson JJ, "The ovariectomized, lactating rat as an experimental model for osteopenia: calcium metabolism and bone changes", Bone Miner, 11, pp.43~53 (1990)
- Bagi CM, "Comparative morphometric changes in rat cortical bone following ovariectomy and", or immobilization, 1993, pp.14, 877~883
- Thompson DD, "FDA Guidelines and animal models for osteoporosis", Bone, 17, pp.125~133 (1995)
- Ohta H, "Influences of menopause and oophorectomy on bone metabolism and spinal bone mineral content", Nihon Sanka Fujinka Gakkai Zasshi, 43, pp.422~428 (1991)
- Sennerby L, "Early tissue response to titanium implants inserted in rabbit cortical bone. Part I. Light microscope observation", J Mater Sci Mater Med, 4, pp.240~250 (1993)
- Masuda T, "Cell and matrix reactions at titanium implants in surgically prepared rat tibiae", Int J Oral Maxillofac Implants, 12, pp.472~485 (1997)
- Lukas D, "Periotest-a dynamic procedure for the diagnosis of the human periodontium", Clin Phys Physiol Meas, 11, pp.65~75 (1990)
- Chen MM, "Effect of ovariectomy on cancellous bone in the hypophysectomized rat", J Bone Miner Res, 10, pp.1334~1342 (1995)