난소 절제술을 시행한 쥐와 정상 쥐에서 합성 골 재료의 이식 후 신생 골 생성 비교
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연구목적: 난소 절제술을 시행하여 골다공증을 재연한 쥐의 두개 결함부에 골 이식시 정상 쥐와 어떠한 골 재생 능력 차이를 보이는지 비교하고자 한다. 연구재료 및 방법: 20마리의 쥐를 2 그룹으로 각 10마리씩 나눈다. 그 중 10마리만 난소 절제술을 시행하고 7주 후 20마리 모두 두개골 에 8 mm 직경의 임계 크기의 결함을 형성한 후 OSTEONTM을 이식한다. 그 후 4주 후 모두 희생하여 조직학적 조각을 형성하여 방사선 학적 및 조직형태학적 분석을 시행한다. 결과: Ovariectomy & OSTEONTM군과 Non-Ovariectomy & OSTEONTM군과의 신생골 형성 넓이의 효과 검증 결과, Ovariectomy & OSTEONTM군은 평균 넓이는 19660.93 ± 17207.68 ㎛으로 Non-Ovariectomy & OSTEONTM군의 69793.57 ± 33227.17 ㎛에 비해 적어진 것으 로 나타났으며, 이러한 차이는 통계적으로도 유의한 것으로 나타났다 (t = -4.237, P < .01). 따라서 Ovariectomy & OSTEONTM군의 new bone formation 넓이가 Non-Ovariectomy & OSTEONTM군에 비해 작아진 것을 알 수 있다. Ovariectomy & OSTEONTM 군과 Non-Ovariectomy & OSTEONTM 군과의 알칼리 포스파타아제의 효과 검증 결과, Ovariectomy & OSTEONTM군은 ALP는 평균 163.90 ± 61.44 IU/L으로 Non-Ovariectomy & OSTEONTM군의 58.40 ± 18.30 IU/L에 비해 크게 높아진 것으로 나타났으며, 이러한 차이는 통계적으로도 유의한 것으로 나타났다 (t = 5.204, P < .001). 따라서 Ovariectomy & OSTEONTM군의 ALP 수치 가 Non-Ovariectomy & OSTEONTM군 ALP 수치보다 높아진 것을 알 수 있다. 결론: 본 실험을 통해 골다공증이 유발되면 골 재생능력이 감소한다는 것을 결론지을 수 있다.
Abstract
Purpose of study: To compare on bone formation between ovariectomized rats and normal rats after graft of alloplastic bone material. Material and methods: Twenty female rats were used in this study. They were divided into two groups by each ten rats. One group was ovariectomized and grafted alloplastic bone material. Other group was non-ovariectomized and grafted alloplastic bone material. After a healing time of 4 weeks, the animals were sacrificed and decalcified preparations were routinely processed for histologic evaluations. Results: Ovariectomy group was significantly lesser bone formation compared with non-ovariectomy group. Conclusions: Ovariectomy acts as a negative factor in new bone formation.
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