Response of osteoblast-like cells cultured on zirconia to bone morphogenetic protein-2
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Purpose: The aim of this study was to compare osteoblast behavior on zirconia and titanium under conditions cultured with bone morphogenetic protein-2. Methods: MC3T3-E1 cells were cultured on sandblasted zirconia and sandblasted/etched titanium discs. At 24 hours after seeding MC3T3-E1, the demineralized bone matrix (DBM) gel alone and the DBM gel with bone morphogenetic protein-2(BMP-2) were added to the culture medium. The surface topography was examined by confocal laser scanning microscopy. Cellular proliferation was measured at 1, 4, and 7 days after gel loading. Alkaline phosphatase activity was measured at 7 days after gel loading. The mRNA expression of ALPase, bone sialoprotein, type I collagen, runt-related transcription factor 2 (Runx-2),osteocalcin, and osterix were evaluated by real-time polymerase chain reaction at 4 days and 7 days. Results: At 1, 4, and 7 days after loading the DBM gel alone and the DBM gel with BMP-2, cellular proliferation on the zirconia and titanium discs was similar and that of the groups cultured with the DBM gel alone and the DBM gel with BMP-2 was not significantly different, except for titanium with BMP-2 gel. ALPase activity was higher in the cells cultured with BMP-2than in the other groups, but there was no difference between the zirconia and titanium. In ALPase, bone sialoprotein, osteocalcin,Runx-2 and osterix gene expression, that of cells on zirconia or titanium with BMP-2 gel was much more highly increased than titanium without gel at day 7. The gene expression level of cells cultured on zirconia with BMP-2 was higher than that on titanium with BMP-2 at day 7. Conclusions: The data in this study demonstrate that the osteoblastic cell attachment and proliferation of zirconia were comparable to those of titanium. With the stimulation of BMP-2, zirconia has a more pronounced effect on the proliferation and differentiation of the osteoblastic cells compared with titanium.
Abstract
Purpose: The aim of this study was to compare osteoblast behavior on zirconia and titanium under conditions cultured with bone morphogenetic protein-2. Methods: MC3T3-E1 cells were cultured on sandblasted zirconia and sandblasted/etched titanium discs. At 24 hours after seeding MC3T3-E1, the demineralized bone matrix (DBM) gel alone and the DBM gel with bone morphogenetic protein-2(BMP-2) were added to the culture medium. The surface topography was examined by confocal laser scanning microscopy. Cellular proliferation was measured at 1, 4, and 7 days after gel loading. Alkaline phosphatase activity was measured at 7 days after gel loading. The mRNA expression of ALPase, bone sialoprotein, type I collagen, runt-related transcription factor 2 (Runx-2),osteocalcin, and osterix were evaluated by real-time polymerase chain reaction at 4 days and 7 days. Results: At 1, 4, and 7 days after loading the DBM gel alone and the DBM gel with BMP-2, cellular proliferation on the zirconia and titanium discs was similar and that of the groups cultured with the DBM gel alone and the DBM gel with BMP-2 was not significantly different, except for titanium with BMP-2 gel. ALPase activity was higher in the cells cultured with BMP-2than in the other groups, but there was no difference between the zirconia and titanium. In ALPase, bone sialoprotein, osteocalcin,Runx-2 and osterix gene expression, that of cells on zirconia or titanium with BMP-2 gel was much more highly increased than titanium without gel at day 7. The gene expression level of cells cultured on zirconia with BMP-2 was higher than that on titanium with BMP-2 at day 7. Conclusions: The data in this study demonstrate that the osteoblastic cell attachment and proliferation of zirconia were comparable to those of titanium. With the stimulation of BMP-2, zirconia has a more pronounced effect on the proliferation and differentiation of the osteoblastic cells compared with titanium.
참고문헌 (19)
- Schliephake H, "Metal release from titanium fixtures during placement in the mandible: an experimental study", Int J Oral Maxillofac Implants, 8, pp.502~511 (1993)
- Urban RM, "Dissemination of wear particles to the liver, spleen, and abdominal lymph nodes of patients with hip or knee replacement", J Bone Joint Surg Am, 82, pp.457~476 (2000)
- Lalor PA, "Sensitivity to titanium. A cause of implant failure", J Bone Joint Surg Br, 73, pp.25~28 (1991)
- Valentine-Thon E, "Validity of MELISA for metal sensitivity testing", Neuro Endocrinol Lett, 24, pp.57~64 (2003)
- Chevalier J, "What future for zirconia as a biomaterial", Biomaterials, 27, pp.535~543 (2006)
- Piconi C, "Zirconia as a ceramic biomaterial", Biomaterials, 201, pp.1~25 (1999)
- Akagawa Y, "Comparison between freestanding and tooth-connected partially stabilized zirconia implants after two years’ function in monkeys: a clinical and histologic study", J Prosthet Dent, 80, pp.551~558 (1998)
- Scarano A, "Bone response to zirconia ceramic implants: an experimental study in rabbits", J Oral Implantol, 29, pp.8~12 (2003)
- Kohal RJ, "Loaded custom-made zirconia and titanium implants show similar osseointegration: an animal experiment", J Periodontol, 75, pp.1262~1268 (2004)
- Depprich R, "Behavior of osteoblastic cells cultured on titanium and structured zirconia surfaces", Head Face Med, 4, pp.29~ (2008)
- Yamashita D, "Effect of surface roughness on initial responses of osteoblast-like cells on two types of zirconia", Dent Mater J, 28, pp.461~470 (2009)
- Kohal RJ, "In vitro reaction of human osteoblasts on alumina-toughened zirconia", Clin Oral Implants Res, 20, pp.1265~1271 (2009)
- Hempel U, "Response of osteoblast-like SAOS-2 cells to zirconia ceramics with different surface topographies", Clin Oral Implants Res, 21, pp.174~181 (2010)
- Suzuki A, "Evidence for a role of p38 MAP kinase in expression of alkaline phosphatase during osteoblastic cell differentiation", Bone, 30, pp.91~98 (2002)
- Sudo H, "In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria", J Cell Biol, 96, pp.191~198 (1983)
- Albrektsson T, "Oral implant surfaces: Part 1--review focusing on topographic and chemical properties of different surfaces and in vivo responses to them", Int J Prosthodont, 17, pp.536~543 (2004)
- Albrektsson T, "Oral implant surfaces: Part 2--review focusing on clinical knowledge of different surfaces", Int J Prosthodont, 17, pp.544~564 (2004)
- He J, "The anatase phase of nanotopography titania plays an important role on osteoblast cell morphology and proliferation", J Mater Sci Mater Med, 19, pp.3465~3472 (2008)
- Bächle M, "Behavior of CAL72 osteoblast-like cells cultured on zirconia ceramics with different surface topographies", Clin Oral Implants Res, 18, pp.53~59 (2007)