본문 바로가기
KERD
논문KCI 등재

골다공증 유발 쥐에서 혈소판 농축 혈장이 골 재생에 미치는 영향

Effect of platelet-rich plasma on bone regeneration in ovariectomized osteoporotic rats

류재준|서규원|강정경|조종문
대한치과보철학회 (The Korean Academy Of Prosthodontics)|2010.01|대한치과보철학회지 (The Journal of Korean Academy of Prosthodontics)|제48권 제01호|pp. 16-27|DOI https://doi.org/10.4047/jkap.2010.48.1.16

1

조회

0

다운로드

1

인용

0

참고문헌

키워드

골다공증Osteoporosis골재생OvariectomyBone regeneration난소절제술임계 크기 결함혈소판 농축 혈장Critical-sized defect (CSD)Platelet-rich plasma (PRP)
주제분류치의학

초록

연구 목적:본 연구의 목적은 실험용 쥐의 초기 골 재생 과정에서 혈소판 농축 혈장 (platelet-rich plasma; PRP)이 난소호르몬 분비 유무에 따라 각각 어떤 효과를 나타내는지 확인 비교해 보는 것이다. 연구 재료 및 방법: 실험용 쥐 40마리 중 20마리에는 난소절제술 (ovariectomy; OVX)을 시행하여 골다공증을 유발시킨 상태에서 골 이식을 하였고, 나머지 20마리에는 난소절제술 없이 골 이식을 하였다. 또, 난소절제술을 시행한 쥐 중 10마리와 난소절제술을 시행하지 않은 쥐 중 10마리에는 골 이식 시 골전도성 합성골 이식재인 MBCPTM (Micro- & macro-porous biphasic calcium phosphate)에 혈소판 농축 혈장을 첨가하여 적용하고, 아래와 같은 실험군으로 구분하였다. A군 10마리의 non - OVX 쥐 / 골 이식재 (MBCPTM). B군 10마리의 non - OVX 쥐 / 골 이식재(MBCPTM) + PRP. C군 10마리의OVX 쥐 / 골 이식재(MBCPTM). D군 10마리의OVX 쥐 / 골 이식재(MBCPTM) + PRP. 모든 실험동물의 두개관 정중부에 직경 8 mm 원형의 임계 크기 결함을 한 개씩 인위적으로 형성한 후, 임계 크기 결함 내부에 골 이식재 및 혈소판 농축 혈장을 적용하여 골 이식술을 실시하였다. 골이식시행4주 후에 실험 동물을 희생시켜 표본을 제작한 후, 광학현미경상을 관찰하고 기존에 형성한 임계 크기 결함 내부에 새롭게 침착된 신생골의 면적을 측정하여 그 측정값을 통계 분석하였다. 결과: 신생골 면적 측정값을 각 군 간 비교하여 다음과 같은 결과를 얻었다. 1. 난소절제술이 시행되지 않은 정상 쥐에서는 혈소판 농축 혈장의 사용이 골 재생에 유의한 효과를 나타내지는 않았다 (P> .05). 2. 난소절제술이 시행된 골다공증 유발 쥐에서는 혈소판 농축 혈장의 사용이 골 재생에 유의한 효과를 나타내었다 (P< .05). 3. 혈소판 농축 혈장이 사용되지 않은 경우, 난소절제술이 시행된 골다공증 유발 쥐는 정상 쥐보다 골 재생 능력이 유의할 만큼 감소하였다 (P< .05). 4. 혈소판 농축 혈장이 사용된 경우, 난소절제술이 시행된 골다공증 유발 쥐라고 하더라도 정상 쥐보다 골 재생 능력이 유의할 만큼 감소하지는 않았다 (P> .05). 결론: 이상의 결과를 토대로, 골전도성 합성골 이식재와 함께 쓰인 혈소판 농축 혈장은 정상 쥐에서보다 난소절제술이 시행된 골다공증 유발 쥐에서 골 재생 및 치유에 더 큰 효과가 있음을 알 수 있었다.

Abstract

Purpose: The aim of this experimental study is to observe the effect of platelet-rich plasma (PRP) on early bone regeneration of rats both in normal condition and in osteoporosisinduced by ovariectomy. Material and methods: Total 40 Sprague-Dawley female rats were divided into 4 groups. A 8-mm-diameter calvarial critical-sized defect (CSD) was made by drilling with trephine at the center of calvaria in cranium of every rat. Every CSD was augmented with an osteoconductive synthetic alloplastic substitute (MBCPTM) and PRP as follows. Group A; 10 non-ovariectomized rats grafted with only MBCPTM. Group B; 10 non-ovariectomized rats grafted with MBCPTM and PRP. Group C; 10 ovariectomized rats grafted with only MBCPTM. Group D; 10 ovariectomized rats grafted with MBCPTM and PRP. At 4 weeks after graft, every rat was sacrificed. And histomorphometric analysis was performed by measuring calcified area of new bone formation within the CSD. Results: Comparing four groups, results were obtained as follows. 1. In non-ovariectomized groups, PRP showed a positive effect somewhat but not significant (P > .05). 2. In ovariectomized groups, PRP showed a positive effect significantly (P < .05). 3. In PRP untreated groups, ovariectomy diminished bone regeneration significantly (P < .05). 4. In PRP treated groups, ovariectomy diminished bone regeneration somewhat but not significant (P > .05). Conclusion: Within the limitation of this study, it can be concluded that PRP in combination with an osteoconductive synthetic alloplastic substitute has an effect on bone regeneration more significantly in ovariectomized osteoporotic rats than in normal rats.

참고문헌 (64)

  1. Lynch SE, "The effects of short-term application of a combination of platelet-derived and insulin-like growth factors on periodontal wound healing", J Periodontol, 62, pp.458~467 (1991)
  2. Terranova VP, "Repopulation of dentin surfaces by periodontal ligament cells and endothelial cells. Effect of basic fibroblast growth factor", J Periodontol, 60, pp.293~301 (1989)
  3. Kiritsy CP, "Role of growth factors in cutaneous wound healing: a review", Crit Rev Oral Biol Med, 4, pp.729~760 (1993)
  4. Giannobile WV, "Periodontal tissue engineering by growth factors", Bone, 19, pp.S23~37 (1996)
  5. Kenley RA, "Biotechnology and bone graft substitutes", Pharm Res, 10, pp.1393~1401 (1993)
  6. Sculean A, "Clinical and histologic evaluation of human intrabony defects treated with an enamel matrix protein derivative (Emdogain)", Int J Periodontics Restorative Dent, 20, pp.374~381 (2000)
  7. Jiang D, "Modification of an osteoconductive anorganic bovine bone mineral matrix with growth factors", J Periodontol, 70, pp.834~839 (1999)
  8. Roberts AB, "Physiological actions and clinical applications of transforming growth factor-beta (TGF-beta)", Growth Factors, 8, pp.1~9 (1993)
  9. Aspenberg P, "Local application of growth-factor IGF-1 to healing bone. Experiments with a titanium chamber in rabbits", Acta Orthop Scand, 60, pp.607~610 (1989)
  10. Marx RE, "Platelet-rich plasma: Growth factor enhancement for bone grafts", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 85, pp.638~646 (1998)
  11. Marx RE, "Platelet-rich plasma: evidence to support its use", J Oral Maxillofac Surg, 62, pp.489~496 (2004)
  12. Sa′nchez AR, "Is platelet-rich plasma the perfect enhancement factor? A current review", Int J Oral Maxillofac Implants, 18, pp.93~103 (2003)
  13. Aghaloo TL, "Investigation of plateletrich plasma in rabbit cranial defects: A pilot study", J Oral Maxillofac Surg, 60, pp.1176~1181 (2002)
  14. Greenhalgh DG, "The role of growth factors in wound healing", J Trauma, 41, pp.159~167 (1996)
  15. Mohan S, "Bone growth factors", Clin Orthop Relat Res, 263, pp.30~48 (1991)
  16. Wergedal JE, "Skeletal growth factor and other growth factors known to be present in bone matrix stimulate proliferation and protein synthesis in human bone cells", J Bone Miner Res, 5, pp.179~186 (1990)
  17. Yim SB, "The effect of platelet rich plasma combined with bovine bone on the treatment of grade II furcation defects in beagle dogs", J Korean Acad Periodontol, 30, pp.257~278 (2000)
  18. Wartiovaara U, "Peripheral blood platelets express VEGF-C and VEGF which are released during platelet activation", Thromb Haemost, 80, pp.171~175 (1998)
  19. Anitua E, "Plasma rich in growth factors: preliminary results of use in the preparation of future sites for implants", Int J Oral Maxillofac Implants, 14, pp.529~535 (1999)
  20. Lozada JL, "Platelet-rich plasma application in sinus graft surgery: Part IBackground and processing techniques", J Oral Implantol, 27, pp.38~42 (2001)
  21. Kassolis JD, "Alveolar ridge and sinus augmentation utilizing platelet-rich plasma in combination with freezedried bone allograft: case series", J Periodontol, 71, pp.1654~1661 (2000)
  22. Wiltfang J, "Sinus floor augmentation with betatricalciumphosphate (beta-TCP): does platelet-rich plasma promote its osseous integration and degradation?", Clin Oral Implants Res, 14, pp.213~218 (2003)
  23. Rodriguez A, "Maxillary sinus augmentation with deproteinated bovine bone and platelet rich plasma with simultaneous insertion of endosseous implants", J Oral Maxillofac Surg, 61, pp.157~163 (2003)
  24. Vercellotti T, "Piezoelectric surgery in implantology: a case report-- a new piezoelectric ridge expansion technique", Int J Periodontics Restorative Dent, 20, pp.358~365 (2000)
  25. Fennis JP, "Mandibular reconstruction: a histological and histomorphometric study on the use of autogenous scaffolds, particulate cortico-cancellous bone grafts and platelet rich plasma in goats", Int J Oral Maxillofac Surg, 33, pp.48~55 (2004)
  26. Fennis JP, "Mandibular reconstruction: a clinical and radiographic animal study on the use of autogenous scaffolds and platelet-rich plasma", Int J Oral Maxillofac Surg, 31, pp.281~286 (2002)
  27. Camargo PM, "Platelet-rich plasma and bovine porous bone mineral combined with guided tissue regeneration in the treatment of intrabony defects in humans", J Periodontal Res, 37, pp.300~306 (2002)
  28. Lekovic V, "Effectiveness of a combination of platelet-rich plasma, bovine porous bone mineral and guided tissue regeneration in the treatment of mandibular grade II molar furcations in humans", J Clin Periodontol, 30, pp.746~751 (2003)
  29. de Obarrio JJ, "The use of autologous growth factors in periodontal surgical therapy: platelet gel biotechnology-case reports", Int J Periodontics Restorative Dent, 20, pp.486~497 (2000)
  30. Choi BH, "Effect of platelet-rich plasma on bone regeneration in autogenous bone graft", Int J Oral Maxillofac Surg, 33, pp.56~59 (2004)
  31. Wiltfang J, "Effects of platelet-rich plasma on bone healing in combination with autogenous bone and bone substitutes in critical-size defects. An animal experiment", Clin Oral Implants Res, 15, pp.187~193 (2004)
  32. Kim SG, "A comparative study of osseointegration of Avana implants in a demineralized freeze-dried bone alone or with platelet-rich plasma", J Oral Maxillofac Surg, 60, pp.1018~1025 (2002)
  33. Kim ES, "Platelet concentration and its effect on bone formation in calvarial defects: an experimental study in rabbits", J Prosthet Dent, 86, pp.428~433 (2001)
  34. Aghaloo TL, "Evaluation of platelet-rich plasma in combination with anorganic bovine bone in the rabbit cranium: a pilot study", Int J Oral Maxillofac Implants, 19, pp.59~65 (2004)
  35. Schlegel KA, "De novo bone formation using bovine collagen and platelet-rich plasma", Biomaterials, 25, pp.5387~5393 (2004)
  36. Whitman DH, "Platelet gel: an autologous alternative to fibrin glue with applications in oral and maxillofacial surgery", J Oral Maxillofac Surg, 55, pp.1294~1299 (1997)
  37. Whitman DH, "A technique for improving the handling of particulate cancellous bone and marrow grafts using platelet gel", J Oral Maxillofac Surg, 56, pp.1217~1218 (1998)
  38. Plachokova AS, "The bone regenerative effect of platelet-rich plasma in combination with an osteoconductive material in rat cranial defects", Clin Oral Implants Res, 17, pp.305~311 (2006)
  39. Fürst G, "Sinus grafting with autogenous plateletrich plasma and bovine hydroxyapatite. A histomorphometric study in minipigs", Clin Oral Implants Res, 14, pp.500~508 (2003)
  40. Plachokova AS, "Early effect of platelet-rich plasma on bone healing in combination with an osteoconductive material in rat cranial defects", Clin Oral Implants Res, 18, pp.244~251 (2007)
  41. Froum SJ, "Effect of platelet-rich plasma on bone growth and osseointegration in human maxillary sinus grafts: three bilateral case reports", Int J Periodontics Restorative Dent, 22, pp.45~53 (2002)
  42. Schaaf H, "Sinus lift augmentation using autogenous bone grafts and platelet-rich plasma: radiographic results", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 106, pp.673~678 (2008)
  43. Schmitz JP, "The biology of platelet-rich plasma", J Oral Maxillofac Surg, 59, pp.1119~1121 (2001)
  44. Riggs BL, "A unitary model for involutional osteoporosis: estrogen deficiency causes both type I and type II osteoporosis in postmenopausal women and contributes to bone loss in aging men", J Bone Miner Res, 13, pp.763~773 (1998)
  45. Acarturk TO, "Commercially available demineralized bone matrix compositions to regenerate calvarial critical-sized bone defects", Plast Reconstr Surg, 118, pp.862~873 (2006)
  46. Schmitz JP, "The critical size defect as an experimental model for craniomandibulofacial nonunions", Clin Orthop Relat Res, 205, pp.299~308 (1986)
  47. Okamura A, "Effect of the difference of bone turnover on peri-titanium implant osteogenesis in ovariectomized rats", J Biomed Mater Res A, 70, pp.497~505 (2004)
  48. Ozawa S, "Ovariectomy hinders the early stage of bone-implant integration: histomorphometric, biomechanical, and molecular analyses", Bone, 30, pp.137~143 (2002)
  49. Cho P, "Examination of the boneimplant interface in experimentally induced osteoporotic bone", Implant Dent, 13, pp.79~87 (2004)
  50. Jakse N, "Influence of PRP on autogenous sinus grafts. An experimental study on sheep", Clin Oral Implants Res, 14, pp.578~583 (2003)
  51. Park SI, "Biologic effect of platelet rich plasma on the initial attachment, proliferation and cellular activity of osteoblast", J Korean Acad Periodontol, 31, pp.513~529 (2001)
  52. Riggs BL, "Overview of osteoporosis", West J Med, 154, pp.63~77 (1991)
  53. von Wowern N, "Osteoporosis: a risk factor in periodontal disease", J Periodontol, 65, pp.1134~1138 (1994)
  54. Hohlweg-Majert B, "Significance of osteoporosis in craniomaxillofacial surgery: a review of the literature", Osteoporos Int, 17, pp.167~179 (2006)
  55. White SC, "Oral radiographic predictors of osteoporosis", Dentomaxillofac Radiol, 31, pp.84~92 (2002)
  56. Kribbs PJ, "Comparison of mandibular bone in normal and osteoporotic women", J Prosthet Dent, 63, pp.218~222 (1990)
  57. Kribbs PJ, "Relationships between mandibular and skeletal bone in an osteoporotic population", J Prosthet Dent, 62, pp.703~707 (1989)
  58. Nagareddy PR, "Withania somnifera improves bone calcification in calcium-deficient ovariectomized rats", J Pharm Pharmacol, 58, pp.513~519 (2006)
  59. Sozer V, "Bone metabolism in ovariectomized rats with induced hyperthyroidism: the effect of estrogen replacement", Chin J Physiol, 49, pp.335~341 (2006)
  60. Midy V, "Vasculotropin", vascular endothelial growth factor induces differentiation in cultured osteoblasts, 1994, pp.199, 380~386
  61. Shui C, "Mild heat shock induces proliferation, alkaline phosphatase activity, and mineralization in human bone marrow stromal cells and Mg-63 cells in vitro", J Bone Miner Res, 16, pp.731~741 (2001)
  62. Augat P, "Mechanics and mechanobiology of fracture healing in normal and osteoporotic bone", Osteoporos Int, 16, pp.S36~43 (2005)
  63. Akinci B, "Low transforming growth factor-beta1 serum levels in idiopathic male osteoporosis", J Endocrinol Invest, 30, pp.350~355 (2007)
  64. Pierce GF, "Platelet-derived growth factor (BB homodimer), transforming growth factor-beta 1, and basic fibroblast growth factor in dermal wound healing. Neovessel and matrix formation and cessation of repair", Am J Pathol, 140, pp.1375~1388 (1992)

관련 논문AI추천

난소 절제술을 시행한 쥐와 정상 쥐에서 합성 골 재료의 이식 후 신생 골 생성 비교류재준, 안재석, 안진수, 이종원대한치과보철학회 · 대한치과보철학회지2008 · 제46권 제4호혈소판 농축 혈장과 혼합된 이종골 이식재(Bio-Oss？)가 가토 두개골 결손부 초기 치유에 미치는 영향김병옥, 김종관, 김흥중, 박주철, 이종우, 임동웅, 장현선대한치주과학회 · Journal of Periodontal & Implant Science2005 · 제35권 제1호비글견 경골에서 임플란트 식립 시 혈소판 농축 혈장이 골형성에 미치는 영향강성수, 국민석, 박홍주, 심경미, 오희균, 유선열, 정승곤대한구강악안면외과학회 · 대한구강악안면외과학회지2010 · 제36권 제2호난소 제거된 쥐의 두개골 결손에서 bisphosphonate와 parathyroid hormone 전신 투여 후 골 재생 평가박경미, 임지영대한통합치과학회 · 대한통합치과학회지2023 · 제12권 제3호난소절제술한 백서에 식립한 임플란트에 대한 PostGraft™의 경구 투여 효과김세원, 조인호, 조춘일, 최유성대한치과보철학회 · 대한치과보철학회지2011 · 제49권 제4호가토 상악동 거상술 후 Bovine Bone (Bio-Oss)과 함께 이식된 혈소판풍부혈장과 혈소판결핍혈장의 골치유능 비교이의석, 이태형, 임재석, 장현석, 정유민, 최용근대한구강악안면외과학회 · 대한구강악안면외과학회지2010 · 제36권 제1호

이 간행물의 다른 논문 (8건)

MAD/MAM을 이용한 치과용 지르코니아 코어의 변연 적합도문홍석, 강동림, 심준성, 이근우pp. 1-7상아질의 봉쇄 시기와 표면처리 방법이 미세인장 결합강도에 미치는 영향강동호, 김선재, 한종현, 박정원pp. 8-15임플란트의 생존율에 영향을 미치는 국소적 인자에 대한 19년간의 후향적 연구김성회, 김선재, 이근우, 한동후pp. 28-40높이가 다른 폐쇄장치에 대한 상악절제술 환자의 만족도이재봉, 임순호, 권호범pp. 41-47임플란트 인상 채득 시 트레이 종류에 따른 인상체의 정확도에 관한 비교 연구이준석, 임종화, 이현정pp. 48-54
원문 보기