비글견 경골에서 임플란트 식립 시 혈소판 농축 혈장이 골형성에 미치는 영향
The effect of platelet rich plasma in bone formation on implant installation in the tibia of beagle dogs
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Introduction: The purpose of this study is to evaluate the effect of platelet rich plasma in bone formation and osseointegration of implant installed in the bone marrow space. Materials and Methods: Five beagle dogs were used as experimental animals. Two implants were installed on each tibia in the dogs. Implants of the control group were installed with no additional graft. Those of the experimental group 1 were installed with autogenous bone graft from the ilium autogenous bone. Platelet rich plasma (PRP) alone was used in experimental group 2 and the mixture of PRP and autogenous bone was used in experimental group 3. The animals were sacrificed at 8 weeks after implantation. The parts of tibia containing implant were harvested and radiographs were taken for radiographic examination. The specimens were prepared for histological examination and histomorphometric analysis of implant-bone contact ratios. Results:1. All implants showed sufficient osseointegration in the cortical bone radiographically and histologically, but osseointergration in the marrow space was not satisfactory. 2. Histomorphometrically, the implant-bone contact ratios in the bone marrow was sequentially high in the experimental group 3 (autogenous bone + PRP group), group 1 (autogenous bone group), group 2 (PRP group), and control group (non-additive). 3. It was verified that there was statistical significance between two experimental groups (group 1 and 3) and the other groups (group 2 and control group). (P<0.005)4. However, there was no statistical significance between group 3 and group 1, also group 2 and control group respectively. Conclusion: These results suggest that platelet rich plasma is effective to osseointegration in the implant installation but there is no statistical significance.
Abstract
Introduction: The purpose of this study is to evaluate the effect of platelet rich plasma in bone formation and osseointegration of implant installed in the bone marrow space. Materials and Methods: Five beagle dogs were used as experimental animals. Two implants were installed on each tibia in the dogs. Implants of the control group were installed with no additional graft. Those of the experimental group 1 were installed with autogenous bone graft from the ilium autogenous bone. Platelet rich plasma (PRP) alone was used in experimental group 2 and the mixture of PRP and autogenous bone was used in experimental group 3. The animals were sacrificed at 8 weeks after implantation. The parts of tibia containing implant were harvested and radiographs were taken for radiographic examination. The specimens were prepared for histological examination and histomorphometric analysis of implant-bone contact ratios. Results:1. All implants showed sufficient osseointegration in the cortical bone radiographically and histologically, but osseointergration in the marrow space was not satisfactory. 2. Histomorphometrically, the implant-bone contact ratios in the bone marrow was sequentially high in the experimental group 3 (autogenous bone + PRP group), group 1 (autogenous bone group), group 2 (PRP group), and control group (non-additive). 3. It was verified that there was statistical significance between two experimental groups (group 1 and 3) and the other groups (group 2 and control group). (P<0.005)4. However, there was no statistical significance between group 3 and group 1, also group 2 and control group respectively. Conclusion: These results suggest that platelet rich plasma is effective to osseointegration in the implant installation but there is no statistical significance.
참고문헌 (36)
- Hutton JE, "Factors related to success and failure rates at 3-year follow-up in a multicenter study of overdentures supported by Branemark implants", Int J Oral Maxillofac Implants, 10, pp.33~42 (1995)
- Jaffin RA, "The excessive loss of Branemark fixtures in type IV bone: a 5-year analysis", J Periodontol, 62, pp.2~4 (1991)
- Marx RE, "Platelet-rich plasma growth factor enhancement for bone grafts", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 85, pp.638~646 (1998)
- Plachokova AS, "Effect of platelet-rich plasma on bone regeneration in dentistry: a systematic review", Clin Oral Implants Res, 19, pp.539~545 (2008)
- Whitman DH, "Platelet gel: an autologous alternative to fibrin glue with applications in oral and maxillofacial surgery", J Oral Maxillofac Surg, 55, pp.1294~1299 (1997)
- Kassolis JD, "Evaluation of the adjunctive benefits of platelet-rich plasma in subantral sinus augmentation", J Craniofac Surg, 16, pp.280~287 (2005)
- Camargo PM, "Platelet-rich plasma and bovine porous bone mineral combined with guided tissue regeneration in the treatment of intrabony defects in humans", J Periodontal Res, 37, pp.300~306 (2002)
- Lekovic V, "Effectiveness of a combination of platelet-rich plasma, bovine porous bone mineral and guided tissue regeneration in the treatment of mandibular grade II molar furcations in humans", J Clin Periodontol, 30, pp.746~751 (2003)
- Fennis JP, "Mandibular reconstruction: a histological and histomorphometric study on the use of autogenous scaffolds, particulate cortico-cancellous bone grafts and platelet rich plasma in goats", Int J Oral Maxillofac Surg, 33, pp.48~55 (2004)
- Nikolidakis D, "The effect of platelet-rich plasma on the bone healing around calcium phosphate-coated and non-coated oral implants in trabecular bone", Tissue Eng, 12, pp.2555~2563 (2006)
- Froum SJ, "Effect of plateletrich plasma on bone growth and osseointegration in human maxillary sinus grafts: three bilateral case reports", Int J Periodontics Restorative Dent, 22, pp.45~53 (2002)
- Monov G, "The effect of platelet-rich plasma upon implant stability measured by resonance frequency analysis in the lower anterior mandibles. A pilot study", Clin Oral Implants Res, 16, pp.461~465 (2005)
- Raghoebar GM, "Does platelet-rich plasma promote remodeling of autologous bone grafts used for augmentation of the maxillary sinus floor?", Clin Oral Implants Res, 16, pp.349~356 (2005)
- Thor A, "Reconstruction of the severely resorbed maxilla with autogenous bone, plateletrich plasma, and implants: 1-year results of a controlled prospective 5-year study", Clin Implant Dent Relat Res, 7, pp.209~220 (2005)
- Plachokova AS, "The bone regenerative effect of platelet-rich plasma in combination with an osteoconductive material in rat cranial defects", Clin Oral Implants Res, 17, pp.305~311 (2006)
- Carlsson L, "Implant fixation improved by close fit. Cylindrical implant-bone interface studied in rabbits", Acta Orthop Scand, 59, pp.272~275 (1988)
- Lekholm U, "Patient selection and preparation. In: Tissue integrated prostheses: osseointegration in clinical dentistry", pp.199~209 (1985), Quintessence
- Esposito M, "Biological factors contributing to failures of osseointegrated oral implants. (II)", Etiopathogenesis. Eur J Oral Sci, 106, pp.721~764 (1998)
- Miyamoto I, "Influence of cortical bone thickness and implant length on implant stability at the time of surgery-clinical, prospective, biomechanical, and imaging study", Bone, 37, pp.776~780 (2005)
- Branemark R, "Biomechanical characterization of osseointegration: an experimental in vivo investigation in the beagle dog", J Orthop Res, 16, pp.61~69 (1998)
- Marx RE, "Platelet-rich plasma: evidence to support its use", J Oral Maxillofac Surg, 62, pp.489~496 (2004)
- Roussy Y, "Activation of human platelet-rich plasmas: effect on growth factors release, cell division and in vivo bone formation", Clin Oral Implants Res, 18, pp.639~648 (2007)
- Marx RE, "Platelet-rich plasma (PRP): what is PRP and what is not PRP?", Implant Dent, 10, pp.225~228 (2001)
- Bauer EA, "Stimulation of in vitro human skin collagenase expression by platelet-derived growth factor", Proc Natl Acad Sci USA, 82, pp.4132~4136 (1985)
- Tzeng DY, "Platelet-derived growth factor promotes human peripheral monocyte activation", Blood, 66, pp.179~183 (1985)
- Miyazono K, "Receptors for transforming growth factor-beta", Adv Immunol, 55, pp.181~220 (1994)
- Canalis E, "Insulin-like growth factor I mediates selective anabolic effects of parathyroid hormone in bone cultures", J Clin Invest, 83, pp.60~65 (1989)
- Jakse N, "Influence of PRP on autogenous sinus grafts. An experimental study on sheep", Clin Oral Implants Res, 14, pp.578~583 (2003)
- Kim SG, "Use of particulate dentin plaster of paris combination with", without platelet-rich plasma in the treatment of bone defects around implants, 2002, pp.17, 86~94
- Butterfield KJ, "Effect of platelet-rich plasma with autogenous bone graft for maxillary sinus augmentation in a rabbit model", J Oral Maxillofac Surg, 63, pp.370~376 (2005)
- Sa′.nchez AR, "Is platelet-rich plasma the perfect enhancement factor?", A current review. Int J Oral Maxillofac Implants, 18, pp.93~103 (2003)
- Anitua E, "New insights into and novel applications for platelet-rich fibrin therapies", Trends Biotechnol, 24, pp.227~234 (2006)
- Hallman M, "Bone substitutes and growth factors as an alternative", complement to autogenous bone for grafting in implant dentistry, 2008, pp.47, 172~192
- Wallace SS, "Effect of maxillary sinus augmentation on the survival of endosseous dental implants. A systematic review", Ann Periodontol, 8, pp.328~343 (2003)
- Schlegel KA, "De novo bone formation using bovine collagen and platelet-rich plasma", Biomaterials, 25, pp.5387~5393 (2004)
- Wiltfang J, "Effects of platelet-rich plasma on bone healing in combination with autogenous bone and bone substitutes in critical-size defects. An animal experiment", Clin Oral Implants Res, 15, pp.187~193 (2004)
