소아 천식에서 유도 객담 내 CXCL13의 의의
Clinical Implication of Sputum CXCL13 in Children with Asthma
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
목 적 : CXCL13은 B 세포의 귀소 반응에 관여하는 케모카인으로 알려져 있으나 최근 T 세포 관련 염증에 관여한다는 보고가 있고, Th17 세포에서 주로 분비된다는 보고도 있다. 이에 본 연구에서는 소아 천식에서 CXCL13의 임상적인 의의와 기관지과민성, 폐기능과 기도 내 호산구성 염증과의 관련성에 대해 알아보고자 하였다. 방 법 : 세브란스 어린이 병원에 내원한 만 6세에서 15세 이하의 소아 160 명을 대상으로 하였다. 천식 환아는 80명, 대조군은 80명이었다. 전체 대상아에서 폐기능 검사, 메타콜린 기관지 유발 검사를 시행하였고, 혈액 내 호산구 수, 혈청 총 IgE, ECP 및 유도객담 내 호산구 수, ECP 및 CXCL13을 측정하였다. 결 과 : 유도 객담 내 CXCL13은 천식군에서 대조군에 비해 의미 있게 높았으며(P=0.003), 객담 내 호산구 수(r=0.190, P=0.017) 및 ECP (r=0.285, P=0.002)와 양의 상관관계를 보였다. 혈액 내 호산구 수 및 ECP, 총 IgE와는 통계적 연관성을 보이지 않았고, 폐기능 및 메타콜린 기관지과민성과도 연관성을 보이지 않았다. 결 론 : CXCL13은 소아 천식에서 호산구성 염증과 관련하여 기도 내 만성 염증에 역할을 담당할 것으로 사료되며, 유도 객담 CXCL13은 향후 케모카인과 천식의 연관성을 연구하는데 도움이 될 것으로 생각된다.
Abstract
Purpose : CXCL13 is known to be a chemokine delivering B cell to the secondary lymphatic follicles, such as spleen, lymph nodes, and Peyer's patches. Recently, there have been some reports that CXCL13 is important for T cell inflammation and expressed by Th17 cell which produces IL-17. This study attempted to examine the clinical implication of CXCL13 in children with asthma. Methods : This study included a total of 160 children aged 6 to 15 years who visited Severance Children's Hospital. There were 80 children with asthma and 80 children without. The pulmonary function test and the methacholine challenge test were performed. Total eosinophil count, eosinophil cationic protein (ECP) and total IgE in serum and eosinophils, ECP and CXCL13 in sputum were measured. Results : The mean age of the subjects was 8.3±2.3 years for the asthma group and 9.3±2.5 years for the control group. The male to female ratio was 67.5% and 55.0% respectively. The sputum CXCL13 level for the asthma group was significantly higher than that level for the control group (P=0.003). This significance persisted even after adjustment for age and sex (P= 0.010). The sputum CXCL13 level showed a positive correlation with that of sputum eosinophils (r=0.190, P=0.017) and ECP (r=0.285, P=0.002). Conclusion : Our results suggest that CXCL13 may play an important role in the pathophysiology of asthma related to eosinophilic inflammation. Further studies on sputum CXCL13 could help to reveal the role of chemokines in asthma.
참고문헌 (25)
- Eder W, "The asthma epidemic", N Engl J Med, 355, pp.2226~2235 (2006)
- 홍수종, "한국 소아알레르기 질환의 유병률", 대한소아알레르기및호흡기학회지, 18(1), pp.15~25 (2008), 대한 소아알레르기 호흡기학회
- Smit JJ, "A closer look at chemokines and their role in asthmatic responses", Eur J Pharmacol, 533, pp.277~288 (2006)
- Holgate ST, "Pathogenesis of asthma", Clin Exp Allergy, 38, pp.872~897 (2008)
- Zimmermann N, "Chemokines in asthma: cooperative interaction between chemokines and IL-13", J Allergy Clin Immunol, 111, pp.227~242 (2003)
- Rothenberg ME, "Chemokines and chemokine receptors: their role in allergic airway disease", J Clin Immunol, 19, pp.250~265 (1999)
- Pease JE, "Asthma, allergy and chemokines", Curr Drug Targets, 7, pp.3~12 (2006)
- Renauld JC, "New insights into the role of cytokines in asthma", J Clin Pathol, 54, pp.577~589 (2001)
- Medoff BD, "T cell trafficking in allergic asthma: the ins and outs", Annu Rev Immunol, 26, pp.205~232 (2008)
- Oliveira SH, "The role of chemokines and chemokine receptors in eosinophil activation during inflammatory allergic reactions", Braz J Med Biol Res, 36, pp.1455~1463 (2003)
- Gunn MD, "A B-cell-homing chemokine made in lymphoid follicles activates Burkitt's lymphoma receptor-1", Nature, 391, pp.799~803 (1998)
- Ansel KM, "A chemokine- driven positive feedback loop organizes lymphoid follicles", Nature, 406, pp.309~314 (2000)
- Kanemitsu N, "CXCL13 is an arrest chemokine for B cells in high endothelial venules", Blood, 106, pp.2613~2618 (2005)
- Ebisuno Y, "Cutting edge: the B cell chemokine CXC chemokine ligand 13", B lymphocyte chemoattractant is expressed in the high endothelial venules of lymph nodes and Peyer's patches and affects B cell trafficking across high endothelial venules, 2003, pp.171, 1642~1646
- Rangel-Moreno J, "Role of CXC chemokine ligand 13, CC chemokine ligand (CCL) 19, and CCL21 in the organization and function of nasal-associated lymphoid tissue", J Immunol, 175, pp.4904~4913 (2005)
- Legler DF, "B cell-attracting chemokine 1, a human CXC chemokine expressed in lymphoid tissues, selectively attracts B lymphocytes via BLR1", CXCR5, 1998, pp.187, 655~660
- Manzo A, "Mature antigen-experienced T helper cells synthesize and secrete the B cell chemoattractant CXCL13 in the inflammatory environment of the rheumatoid joint", Arthritis Rheum, 58, pp.3377~3387 (2008)
- Schaerli P, "CXC chemokine receptor 5 expression defines follicular homing T cells with B cell helper function", J Exp Med, 192, pp.1553~1562 (2000)
- Takagi R, "B cell chemoattractant CXCL13 is preferentially expressed by human Th17 cell clones", J Immunol, 181, pp.186~189 (2008)
- Masoli M, "The global burden of asthma: executive summary of the GINA Dissemination Committee report", Allergy, 59, pp.469~478 (2004)
- Crapo RO, "Guidelines for methacholine and exercise challenge testing-1999. This official statement of the American Thoracic Society was adopted by the ATS Board of Directors, July 1999", Am J Respir Crit Care Med, 161, pp.309~329 (2000)
- Yoon KA, "Normal predicted values of pulmonary function test in Korean school-aged children", J Korean Pediatr Soc, 36, pp.25~37 (1993)
- Yoshikawa T, "Severity of exercise-induced bronchoconstriction is related to airway eosinophilic inflammation in patients with asthma", Eur Respir J, 12, pp.879~884 (1998)
- Fulkerson PC, "Pulmonary chemokine expression is coordinately regulated by STAT1, STAT6, and IFN-gamma", J Immunol, 173, pp.7565~7574 (2004)
- Jenh CH, "Human B cell-attracting chemokine 1 (BCA-1; CXCL13) is an agonist for the human CXCR3 receptor", Cytokine, 15, pp.113~121 (2001)