국내 유통되는 인플루엔자 백신에서 ELISA에 의한 계란 단백의 정량적 비교
Quantitative Analysis of Egg Protein by ELISA in Distributed Influenza Vaccine in Korea
2
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
목 적 : 인플루엔자는 매년 중대한 영향을 끼치는 질병이므로 특히 소아와 같은 고위험군에서는 인플루엔자 예방접종이 강조된다. 인플루엔자 백신은 계란 단백을 함유하므로 계란 알레르기 환자에게 투여시 심각한 부작용을 우려하여 적극적인 백신투여가 이루어지지 못한다. 따라서 본 연구에서는 국내 유통되는 인플루엔자 백신의 계란 단백의 정량을 분석하고자 한다. 방 법 : 2004년, 2005년, 2006년에 국내 유통되고 있는 인플루엔자 백신을 각 회사별로 같은 lot 번호별로, 다른 lot 번호별로 준비하여 각 백신에 포함된 ovalbumin이 coating되어 있는 microtiter plates에 백신 원액부터 2배로 단계 희석한 백신과 ovalbumin을 분주하였고, 2차 항체로 horse radish peroxidase-conjugate rabbit anti-ovalbumin을 분주하였다. 450 nm에서 흡광도를 측정한 후 standard curve를 통해 ng/mL로 변환시켰다. 결 과 : 백신에 가장 많이 측정된 ovalbumin 농도는 101.24 ng/mL였고, 가장 낮은 농도는 21.23 ng/mL였다. ovalbumin 농도는 제약 회사간 차이를 보였고, 같은 제조회사에서 같은 년도에 제작되었지만 다른 lot 번호를 가진 백신의 ovalbumin 농도가 모두 달랐으며 같은 lot 번호를 가진 백신에서도 ovalbumin 농도는 차이를 보였다. 결 론 : 본 인구에서 조사한 국내 유통되고 있는 백신에 함유되어 있는 ovalbumin의 양은 모두 1.2 µg/mL 이하로 측정되었다. 따라서 심각한 계란 알레르기가 있는 환자에서 본 연구 결과는 인플루엔자 백신 접종시 참고자료가 될 수 있겠다.
Abstract
Purpose : Influenza infection is a major public health concern, especially in young children and in high risk groups such as patients. Traditionally, influenza vaccines are produced from infertilized eggs. Therefore, patients with severe egg allergy can show adverse reactions after vaccination and be dalayed in active immunization. The aim of this study was to determine the ovalbumin concentration of a commercially available influenza vaccines in Korea. Methods : The ovalbumin content in each vaccine was determined by enzyme linked immunosorbent assay. The ovalbumin content was compared among the different manufactures, the different lot numbers and the same lot numbers of vaccines. The microtiter plates coated with goat anti-chicken egg albumin antibody were inoculated with serial diluted vaccines and ovalbumin. After incubation with horse radish peroxidase-conjugate rabbit anti-ovalbumin, absorbance was read at 450 nm and fitted to the standard curve. Results : The highest ovalbumin content was 101.24 ng/mL, and the lowest ovalbumin content was 21.23 ng/mL. The amount of ovalbumin differed not only among the different vaccines, but also different lot numbers of individual vaccines and even the same lot numbers of vaccines. Conclusion : In none of the vaccines tested in our study, ovalbumin content reached the maximum values for ovalbumin (1.2 µg/mL). Therefore, in severe egg allergic patients, our results provide the useful information on influenza vaccination.
참고문헌 (28)
- Fukuda K, "Inactivated influenza vaccines", Vaccines, pp.348~349
- CDC, "Prevention and control of influenza: recommendation of the advisory committee on immunization practice (ACIP)", MMWR Morb Mortal Wkly Rep, 57, pp.1~60 (2008)
- Esposito S, "Safe administration of an inactivated virosomal adjuvanted influenza vaccine in asthmatic children with egg allergy", Vaccine, 26, pp.4664~4668 (2008)
- Kemp AS, "Egg allergy", Pediatr Allergy Immunol, 18, pp.696~702 (2007)
- Madaan A, "Vaccine allergy: diagnosis and management", Immunol Allergy Clin North Am, 23, pp.555~588 (2003)
- Neuzil KM, "The effect of influenza on hospitalizations, outpatient visits and courses of antibiotics in children", N Eng J Med, 342, pp.225~231 (2000)
- Kondo S, "The effects of influenza virus infection on FEV1 in asthmatic children. the time-course study", Chest, 100, pp.1235~1238 (1991)
- Neuzil KM, "The burden of influenza illness in children with asthma and other chronic medical conditions", J Pediatr, 137, pp.856~864 (2000)
- Salk JE, "A clinical, epidemiological and immunological evaluation of vaccination against epidemic influenza", Am J Hyg, 42, pp.57~93 (1945)
- Edwards KM, "A randomized controlled trial of cold-adapted and inactivated vaccines for the prevention of influenza A disease", J Infect Dis, 169, pp.68~76 (1994)
- Nichol KL, "The effectiveness of vaccination against influenza in healthy, working adults", N Engl J Med, 333, pp.889~893 (1995)
- Neuzil KM, "Efficacy of inactivated and cold- adapted vaccines against influenza A infection, 1985 to 1990: the pediatric experience", Pediatr Infect Dis J, 20, pp.733~740 (2001)
- Hoberman A, "Efficacy of inactivated influenza vaccine in preventing acute otitis media in children", JAMA, 290, pp.1608~1616 (2003)
- Sugaya N, "Efficacy of inactivated vaccine in preventing antigenically drifted influenza type A and well matched type B", JAMA, 272, pp.1122~1126 (1994)
- Bell TD, "Immunization with killed influenza virus in children with chronic asthma", Chest, 73, pp.140~145 (1978)
- Kramarz P, "Does influenza vaccination prevent asthma exacerbations in children?", J Pediatr, 138, pp.306~310 (2001)
- Edevg G, "The development and standardization of an ELISA for ovalbumin determination in influenza vaccines", J Biol Stand, 14, pp.223~230 (1986)
- Eggesbo M, "The prevalence of allergy to egg: a population based study in young children", Allergy, 56, pp.403~411 (2001)
- Roberts G, "Relationship between aeroallergen and food allergen sensitization in childhood", Clin Exp Allergy, 35, pp.933~940 (2005)
- Sampson HA, "Relationship between food specific IgE concentrations and the risk of positive food challenges in children and adolescents", J Allergy Clin Immunol, 100, pp.444~451 (1997)
- Zeiger RS, "Current issues with influenza vaccination in egg allergy", J Allergy Clin Immunol, 110, pp.834~840 (2002)
- Report of the Committee on Infectious Diseases ed 27, "Pickering LK", American Academy of Pediatrics, ~ (2006)
- Ratner B, "Allergy to virus and rickettsial vaccines. I", Allergy to influenza A and B vaccine JAMA, 132, pp.899~905 (1946)
- Bierman CW, "Safety of influenza vaccination in allergic children", J Infect Dis, 136, pp.652~655 (1977)
- Stull CA, "A study to determine whether chick egg yolk sac vaccines contained sufficient egg proteins to cause severe systemic reactions if given to egg sensitive individuals", J Immunol, 53, pp.343~351 (1946)
- O'Brien TC, "Quantitation of residual host protein in chicken embryo-derived vaccines by radial immunodiffusion", Appl Microbiol, 21, pp.780~782 (1971)
- Davies R, "Egg allergy, influenza vaccine, and immunoglobulin E antibody", J Allergy Clin Immunol, 57, pp.373~383 (1976)
- Chaloupka I, "Comparative analysis of six European influenza vaccines", Eur J Clin Microbiol Infect Dis, 15, pp.121~127 (1996)