소아 갈치알레르기: 대구 특이 IgE 검사 활용성 및 대구와의 교차반응
Cutlassfish allergy in children: usefulness of serum cod specific IgE and cross-reaction with cod
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Purpose: Fish allergy is the ninth common food allergy, and cutlassfish is one of the common allergenic fishes in Korean children. However, there is no commercial diagnostic tool for testing cutlassfish allergy in the world. We evaluated the usefulness of serum cod specific IgE (cod-sIgE) to diagnose cutlassfish allergy and cross-reaction between cutlassfish and cod. Methods: Nineteen children who experienced immediate type reactions after consumption of cutlassfish were enrolled. Cod-sIgE was measured by ImmunoCAP, and serum samples were obtained from 11 allergic patients and 11 controls. Using our own homemade crude extracts, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), anti-parvalbumin (PV) immunoglobulin G immunoblot, enzyme-linked immunosorbent assay (ELISA), and ELISA inhibition were performed. Results: Thirteen patients were clinically allergic to both cutlassfish and cod, and 6 were allergic to cutlassfish alone. The median age and cod-sIgE concentrations were not significantly different between the 2 groups. The clear fish protein bands and PVs were identified on SDS-PAGE and immunoblotting. Serum cod-sIgE was positive in 4 out of 6 cutlassfish mono-allergic patients, however, there was no significant correlation between cod-sIgE by ImmunoCAP and cutlassfish-specific IgE by ELISA. The cutlassfish IgE ELISA was profoundly inhibited by cutlassfish, while the cod IgE ELISA was profoundly inhibited by cod but partially inhibited by cutlassfish. Conclusion: We found a potential diagnostic value of cod-sIgE to diagnose cutlassfish allergy and the asymmetric cross-reaction between cutlassfish and cod. These results could help diagnose and provide a dietary guidance in cutlassfish allergic children.
Abstract
Purpose: Fish allergy is the ninth common food allergy, and cutlassfish is one of the common allergenic fishes in Korean children. However, there is no commercial diagnostic tool for testing cutlassfish allergy in the world. We evaluated the usefulness of serum cod specific IgE (cod-sIgE) to diagnose cutlassfish allergy and cross-reaction between cutlassfish and cod. Methods: Nineteen children who experienced immediate type reactions after consumption of cutlassfish were enrolled. Cod-sIgE was measured by ImmunoCAP, and serum samples were obtained from 11 allergic patients and 11 controls. Using our own homemade crude extracts, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), anti-parvalbumin (PV) immunoglobulin G immunoblot, enzyme-linked immunosorbent assay (ELISA), and ELISA inhibition were performed. Results: Thirteen patients were clinically allergic to both cutlassfish and cod, and 6 were allergic to cutlassfish alone. The median age and cod-sIgE concentrations were not significantly different between the 2 groups. The clear fish protein bands and PVs were identified on SDS-PAGE and immunoblotting. Serum cod-sIgE was positive in 4 out of 6 cutlassfish mono-allergic patients, however, there was no significant correlation between cod-sIgE by ImmunoCAP and cutlassfish-specific IgE by ELISA. The cutlassfish IgE ELISA was profoundly inhibited by cutlassfish, while the cod IgE ELISA was profoundly inhibited by cod but partially inhibited by cutlassfish. Conclusion: We found a potential diagnostic value of cod-sIgE to diagnose cutlassfish allergy and the asymmetric cross-reaction between cutlassfish and cod. These results could help diagnose and provide a dietary guidance in cutlassfish allergic children.
참고문헌 (18)
- Kamdar TA, "Prevalence and characteristics of adult-onset food allergy", J Allergy Clin Immunol Pract, 3, pp.114~115 (2015)
- Lee E, "Causes of food allergy according to age and severity : a recent 10-year retrospective study from a single tertiary hospital", Allergy Asthma Respir Dis, 8, pp.80~88 (2020)
- Buyuktiryaki B, "IgE-mediated fish allergy in children", Medicina, 57, pp.76~ (2021)
- Sharp MF, "Fish allergy : in review", Clin Rev Allergy Immunol, 46, pp.258~271 (2014)
- Pascual CY, "Fish allergy in childhood", Pediatr Allergy Immunol, 19, pp.573~579 (2008)
- Youm S, "White meat fish allergy in Korean children : a single hospital based retrospective study", Allergy Asthma Repir Dis, 12, pp.72~77 (2024)
- Lim DLC, "Parvalbumin–the major tropical fish allergen", Pediatr Allergy Immunol, 19, pp.399~407 (2008)
- Stephen JN, "Allergenicity of bony and cartilaginous fish-molecular and immunological properties", Clin Exp Allergy, 47, pp.300~312 (2017)
- Yuk JE, "Allergenicity and stability of 6 new Korean bony fish extracts", Allergy Asthma Immunol Res, 13, pp.623~637 (2021)
- Hilger C, "Diagnosis of allergy to mammals and fish: cross-reactive vs. specific markers", Curr Allergy Asthma Rep, 17, pp.64~ (2017)
- Cox AL, "Clinical relevance of cross-reactivity in food", J Allergy Clin Immunol Pract, 9, pp.82~99 (2021)
- Schulkes KJ, "Specific IgE to fish extracts does not predict allergy to specific species within an adult fish allergic population", Clin Transl Allergy, 4, pp.27~ (2014)
- Aalberse RC, "Assessment of allergen cross reactivity", Clin Mol Allergy, 5, pp.2~ (2007)
- Van Do T, "Allergy to fish parvalbumins : studies on the cross-reactivity of allergens from 9 commonly consumed fish", J Allergy Clin Immunol, 116, pp.1314~1320 (2005)
- Beale JE, "Characterisation of purified parvalbumin from five fish species and nucleotide sequencing of this major allergen from Pacific pilchard, Sardinops sagax", Mol Immunol, 46, pp.2985~2993 (2009)
- Swoboda I, "Recombinant carp parvalbumin, the major cross-reactive fish allergen : a tool for diagnosis and therapy of fish allergy", J Immunol, 168, pp.4576~4584 (2002)
- Bugajska-Schretter A, "Parvalbumin, a cross-reactive fish allergen, contains IgE-binding epitopes sensitive to periodate treatment and Ca2+ depletion", J Allergy Clin Immunol, 101, pp.67~74 (1998)
- Sicherer SH, "Food allergy", J Allergy Clin Immunol, 125(2 Suppl 2), pp.S116~S125 (2010)