Backbone NMR Assignments and Secondary Structure Determination of a Cupin-family Protein YaiE from Escherichia coli
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Cupin-superfamily proteins represent the most functionally diverse groups of proteins and include a huge number of functionally uncharacterized proteins. Recently, YaiE, a cupin protein from Escherichia coli has been suggested to be involved in a novel activity of pyrimidine/purine nucleoside phosphorylase (PPNP). In the present study, we achieved a complete backbone NMR assignments of YaiE, by a series of heteronuclear multidimensional NMR experiments on its [13C/15N]-enriched sample. Subsequently, secondary structure analysis using the assigned chemical shift values identified 10 obvious β-strands and a tentative 310-helix. Taken all together, the results constitute the first structural characterization of a putative PPNP cupin protein.
Abstract
Cupin-superfamily proteins represent the most functionally diverse groups of proteins and include a huge number of functionally uncharacterized proteins. Recently, YaiE, a cupin protein from Escherichia coli has been suggested to be involved in a novel activity of pyrimidine/purine nucleoside phosphorylase (PPNP). In the present study, we achieved a complete backbone NMR assignments of YaiE, by a series of heteronuclear multidimensional NMR experiments on its [13C/15N]-enriched sample. Subsequently, secondary structure analysis using the assigned chemical shift values identified 10 obvious β-strands and a tentative 310-helix. Taken all together, the results constitute the first structural characterization of a putative PPNP cupin protein.
참고문헌 (14)
- J. M. Dunwell, , Phytochemistry, 65, pp.7~ (2004)
- R. Uberto, , PLoS One, 8, pp.e74477~ (2013)
- D. C. Sévin, , Nat. Methods, 14, pp.187~ (2017)
- M. J. Pugmire, , Biochem. J, 361, pp.1~ (2002)
- R. G. Silva, , Curr. Drug Targets, 8, pp.413~ (2007)
- G. Liechti, , J. Bacteriol, 194, pp.839~ (2012)
- F. Delaglio, , J. Biomol. NMR, 6, pp.277~ (1995)
- B. A. Johnson, , Methods Mol. Biol, 278, pp.313~ (2004)
- D. S. Wishart, , J. Biomol. NMR, 4, pp.171~ (1994)
- Y. Shen, , J. Biomol. NMR, 56, pp.227~ (2013)
- 이유섭, "Backbone NMR Assignments of an Uncharacterized Protein, SF1002 from Shigella flexneri 5a M90T", Journal of the Korean Magnetic Resonance Society, 19(1), pp.36~41 (2015), 한국자기공명학회[DOI]
- Dae-Won Sim, "Backbone NMR assignments of a putative secretory protein from Helicobacter pylori, using ahigh - field (900MHz) NMR", Journal of the Korean Magnetic Resonance Society, 13(2), pp.108~116 (2009), 한국자기공명학회
- 심대원, "HP0902 from Helicobacter pylori is a thermostable, dimeric protein belonging to an all-β topology of the cupin superfamily", BMB reports, 42(6), pp.387~392 (2009), 한국생화학분자생물학회
- D. W. Sim, , FEBS Lett, 590, pp.2997~ (2016)