加味歸脾湯이 P815 세포의 serotonin 대사 과정에 미치는 영향
Study on Effect to Serotonin Metabolism of Gamiguibi-tang on P815 Cell
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Objectives :This study was perfomed to investigate the antioxidant activity and serotonin activity of Gami-guibi-tang on P815 Mast Cell. Methods :The effects of Gami-guibi-tang on activation of TPH-1 mRNA and AAADC mRNA in P815 mast cell were investigated. The effect of Gami-guibi-tang on content of serotonin in P815 mast cell was investigated. The effects of Gami-guibi-tang on activation of DPPH radical‐scavenging and SOD in P815 mast cell were investigated. Results :1. The Gami-guibi-tang increased SOD activity and DPPH radical‐scavenging activity. 2. The Gami-guibi-tang increased the intracellular concentration of serotonin in 60 ㎍/㎖, 80 ㎍/㎖ experiment group. 3. The Gami-guibi-tang increased menaingful the manifestation TPH mRNA. 4. The manifestation of AAADC and MAO mRNA have not made menaingful changes on Gami-guibi-tang. Conclusions :This experiment shows that Gami-guibi-tang had significant anti-oxidative effect. And Gami-guibi-tang increased the intracellular concentration of serotonin. Therefore, Gami-guibi-tang can be used by the medication of major depression disorder. But Study on mechanism of increased serotonin and clinical research of Gami-guibi-tang is suggested for future research.
Abstract
Objectives :This study was perfomed to investigate the antioxidant activity and serotonin activity of Gami-guibi-tang on P815 Mast Cell. Methods :The effects of Gami-guibi-tang on activation of TPH-1 mRNA and AAADC mRNA in P815 mast cell were investigated. The effect of Gami-guibi-tang on content of serotonin in P815 mast cell was investigated. The effects of Gami-guibi-tang on activation of DPPH radical‐scavenging and SOD in P815 mast cell were investigated. Results :1. The Gami-guibi-tang increased SOD activity and DPPH radical‐scavenging activity. 2. The Gami-guibi-tang increased the intracellular concentration of serotonin in 60 ㎍/㎖, 80 ㎍/㎖ experiment group. 3. The Gami-guibi-tang increased menaingful the manifestation TPH mRNA. 4. The manifestation of AAADC and MAO mRNA have not made menaingful changes on Gami-guibi-tang. Conclusions :This experiment shows that Gami-guibi-tang had significant anti-oxidative effect. And Gami-guibi-tang increased the intracellular concentration of serotonin. Therefore, Gami-guibi-tang can be used by the medication of major depression disorder. But Study on mechanism of increased serotonin and clinical research of Gami-guibi-tang is suggested for future research.
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