LC-MS/MS에 의한 하천수중 스트렙토마이신 분석
The Analysis of Residual Streptomycin in Surface Water by Liquid Chromatography-Tandem Mass Spectrometry
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초록
The method for the determination of streptomycin in environmental aqueous water samples by LC/ESI-MS/MS was developed. After ion pairing reagent was added into surface water sample, aqueous samples were extracted and concentrated through a Hydrophilic-Lipophilic Balanced (HLB) cartridge. In reverse phase liquid chromatographic (RPLC) system, chromatographic separation was achieved on a Eclipse Plus C18 column (2.1×100 mm, 5 μm), using gradient elution with 20 mM HFBA in water and acetonitrile as mobile phase. After HPLC separation, quantification and qualification were performed by electrospray ionization(ESI) and tandem mass spectrometry. As a result, calibration curve showed good linearities (above r2=0.99) in the concentration range of 0.25~1.0 μg/L. From the spiked blank surface water, the limit of detection (LOD) and the limit of quantitation (LOQ) were 0.07 μg/L and 0.23 μg/L, respectively and recovery was the range of 48.7~76%. The established method can be used to determine μg/L levels of streptomycin from the environmental aqueous sample, and also can be applied to the other environmental samples.
Abstract
The method for the determination of streptomycin in environmental aqueous water samples by LC/ESI-MS/MS was developed. After ion pairing reagent was added into surface water sample, aqueous samples were extracted and concentrated through a Hydrophilic-Lipophilic Balanced (HLB) cartridge. In reverse phase liquid chromatographic (RPLC) system, chromatographic separation was achieved on a Eclipse Plus C18 column (2.1×100 mm, 5 μm), using gradient elution with 20 mM HFBA in water and acetonitrile as mobile phase. After HPLC separation, quantification and qualification were performed by electrospray ionization(ESI) and tandem mass spectrometry. As a result, calibration curve showed good linearities (above r2=0.99) in the concentration range of 0.25~1.0 μg/L. From the spiked blank surface water, the limit of detection (LOD) and the limit of quantitation (LOQ) were 0.07 μg/L and 0.23 μg/L, respectively and recovery was the range of 48.7~76%. The established method can be used to determine μg/L levels of streptomycin from the environmental aqueous sample, and also can be applied to the other environmental samples.
참고문헌 (12)
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