LC/MS/MS를 이용한 축산밀집지역 하천에서 테트라사이클린계 항생제 및 대사체 분석 연구
Analysis of Tetracycline Antibiotics and their Metabolites Sampled from Streams near Concentrated Livestock Operations Using LC/MS/MS
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초록
The purpose of this study was to optimize an analytical method for the detection of tetracycline antibiotics and their metabolites in water and to determine their fate and movement in streams. To analyze three tetracycline antibiotics and eight of their metabolites, water quality samples were taken from nine sites, which were scattered across upstream areas, into which livestock manure was directly released from nearby livestock operations, to downstream areas, between November 2010 and October 2011. These samples were pretreated with hydrophilic-lipophilic balance (HLB; 200 mg, 6 cc) cartridges and analyzed using liquid chromatography/ tandem mass spectrometry (LC/MS/MS). Consequently, the limit of detection of the analysis method for the tetracycline antibiotics and their metabolites ranged from 3.7 to 18.2 ng/L. The recovery rates were 81-122%, and the relative standard deviation was less than 16%. In addition, the concentration of tetracycline antibiotics and their metabolites decreased or they were undetected when sampled from the upstream to downstream sites. Although the levels of tetracycline antibiotics and their metabolites detected were lower than their EC50, it is necessary to continue monitoring these substances in the environment.
Abstract
The purpose of this study was to optimize an analytical method for the detection of tetracycline antibiotics and their metabolites in water and to determine their fate and movement in streams. To analyze three tetracycline antibiotics and eight of their metabolites, water quality samples were taken from nine sites, which were scattered across upstream areas, into which livestock manure was directly released from nearby livestock operations, to downstream areas, between November 2010 and October 2011. These samples were pretreated with hydrophilic-lipophilic balance (HLB; 200 mg, 6 cc) cartridges and analyzed using liquid chromatography/ tandem mass spectrometry (LC/MS/MS). Consequently, the limit of detection of the analysis method for the tetracycline antibiotics and their metabolites ranged from 3.7 to 18.2 ng/L. The recovery rates were 81-122%, and the relative standard deviation was less than 16%. In addition, the concentration of tetracycline antibiotics and their metabolites decreased or they were undetected when sampled from the upstream to downstream sites. Although the levels of tetracycline antibiotics and their metabolites detected were lower than their EC50, it is necessary to continue monitoring these substances in the environment.
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