Comparative Analysis of Antioxidant, Antibacterial, and Anti-inflammatory Effects as Cosmetic Ingredients of Sargassum Yezoense Extracts: NF-κB and JAK/STAT Signaling Pathways
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Seaweed is rich in nutrients such as dietary fiber, amino acids, and minerals and it is consumed in various Asian countries, including South Korea. Recently, seaweed has been utilized as a raw material for products such as health supplements for pets, cosmetics, and eco-friendly health foods. However, research on seaweed, being marine-based, is relatively limited compared to terrestrial plants. This study evaluated the potential of Sargassum yezoense (S. yezoense), belonging to the Sargassaceae family of brown algae, extracted using distilled water (SYW) and 70% ethanol (SYE), as a cosmetic ingredient. Antioxidant effect of SYE was higher than that of SYW. Through the paper disc diffusion assay, the antibacterial effects against C. acnes, S. epidermidis, S. aureus, E. coli, and P. aeruginosa were evaluated. In all strains, the antibacterial effect of SYE was higher than SYW. Both extracts showed a cell viability of more than 80% at 50 μg/ml in RAW 264.7 cells, and NO production was significantly reduced by SYE compared to SYW in LPS-induced RAW 264.7 cells. Furthermore, protein and mRNA expression of iNOS and COX-2 were significantly reduced by SYE compared to SYW in LPS-induced RAW 264.7 cells. SYE inhibited the activation of NFκB and JAK/STAT signaling pathways. Based on these results, it suggests that SYE has more applicability as a cosmetic material than SYW.
Abstract
Seaweed is rich in nutrients such as dietary fiber, amino acids, and minerals and it is consumed in various Asian countries, including South Korea. Recently, seaweed has been utilized as a raw material for products such as health supplements for pets, cosmetics, and eco-friendly health foods. However, research on seaweed, being marine-based, is relatively limited compared to terrestrial plants. This study evaluated the potential of Sargassum yezoense (S. yezoense), belonging to the Sargassaceae family of brown algae, extracted using distilled water (SYW) and 70% ethanol (SYE), as a cosmetic ingredient. Antioxidant effect of SYE was higher than that of SYW. Through the paper disc diffusion assay, the antibacterial effects against C. acnes, S. epidermidis, S. aureus, E. coli, and P. aeruginosa were evaluated. In all strains, the antibacterial effect of SYE was higher than SYW. Both extracts showed a cell viability of more than 80% at 50 μg/ml in RAW 264.7 cells, and NO production was significantly reduced by SYE compared to SYW in LPS-induced RAW 264.7 cells. Furthermore, protein and mRNA expression of iNOS and COX-2 were significantly reduced by SYE compared to SYW in LPS-induced RAW 264.7 cells. SYE inhibited the activation of NFκB and JAK/STAT signaling pathways. Based on these results, it suggests that SYE has more applicability as a cosmetic material than SYW.
참고문헌 (48)
- 강갑석, "김치첨가에 따른 해물만두의 저장 효과", 한국식품저장유통학회지, 8(4), pp.393~398 (2001)
- 김미선, "35종 해조류 추출물의 병원성 세균 및 Candida sp. 진균에 대한 항균 활성 평가", 한국미생물·생명공학회지, 40(2), pp.144~151 (2012), 한국미생물·생명공학회[DOI]
- 옥정현, "한국산 갈조식물 모자반속(Sargassum)의 분류 II.Bactrophycus아속 Halochloa와 Repentia절", ALGAE, 21(4), pp.393~405 (2006), 한국조류학회I
- 전의상, "한국에서 자생하는 갈조식물문에 관한 본초학적 연구", 대한본초학회지, 36(4), pp.9~22 (2021), 대한본초학회
- 허윤정, "뷰티 소비시장의 변화에 따른 비건 화장품 매칭 융합 서비스 애플리케이션 개발 연구", 한국과학예술융합학회, 39(3), pp.505~517 (2021), 한국전시산업융합연구원[DOI]
- 홍인선, "갈조류 왜모자반(Sargassum yezoense)의 계절에 따른 형태변화와 생활사", 한국자연보호학회지, 2(2), pp.132~139 (2008)
- Achterman, R. R., "A foot in the door for dermatophyte research", PLoS pathogens, 8(3), pp.e1002564~ (2012)
- 안선미, "해조류 추출물의 항산화 및 nitrite 소거활성 평가", 생명과학회지, 21(4), pp.576~583 (2011), 한국생명과학회
- Alsamarai, A. M., "Association between stress and skin disease", Middle East Journal of Internal Medicine, 3(1) (2010)
- Basavaraj, K., "Diet in dermatology : present perspectives", Indian journal of dermatology, 55(3), pp.205~ (2010)
- Benabbas, R., "Development and pharmaceutical performance of a novel co-processed excipient of alginic acid and microcrystalline cellulose", Powder technology, 378, pp.576~584 (2021)
- Briganti, S., "Antioxidant activity, lipid peroxidation and skin diseases. What’s new", Journal of the European Academy of Dermatology and Venereology, 17(6), pp.663~669 (2003)
- Byrd, A. L., "The human skin microbiome", Nature Reviews Microbiology, 16(3), pp.143~155 (2018)
- Chen, P., "Potential role of the skin microbiota in Inflammatory skin diseases", Journal of Cosmetic Dermatology, 20(2), pp.400~409 (2021)
- Davidovici, B. B., "Psoriasis and systemic inflammatory diseases : potential mechanistic links between skin disease and co-morbid conditions", Journal of Investigative Dermatology, 130(7), pp.1785~1796 (2010)
- Fonacier, L. S., "Allergic skin diseases", Journal of Allergy and Clinical Immunology, 125(2), pp.S138~S149 (2010)
- Generalić Mekinić, I., "Phenolic content of brown algae(Pheophyceae)species : Extraction, identification, and quantification", Biomolecules, 9(6), pp.244~ (2019)
- George, G., "Downregulation of TLR4", MyD88, STAT pathway in RAW 264. 7 cells by Alpinia galanga reveals its beneficial effects in inflammation, pp.Journal of Ethnopharmacology, 2021[DOI]
- Haftcheshmeh, S. M., "Berberine as a natural modulator of inflammatory signaling pathways in the immune system : Focus on NF-κB, JAK", STAT, and MAPK signaling pathways, 2022, pp.36(3), 1216~1230
- Hamdy, D., "Recent use of natural thickeners in the printing process", Journal of Textiles, Coloration and Polymer Science, 18(2), pp.75~81 (2021)
- Han, H.-S., "Cirsimarin, a flavone glucoside from the aerial part of Cirsium japonicum var. ussuriense(Regel)Kitam. ex Ohwi, suppresses the JAK", STAT and IRF-3signaling pathway in LPS-stimulated RAW 264. 7macrophages, 2018, pp.293, 38~47
- 황은경, "Seaweed cultivation and utilization of Korea", ALGAE, 35(2), pp.107~121 (2020), 한국조류학회I[DOI]
- Jeffrey, B. K., "<em>Staphylococcus epidermidis<", em> enables <em>Cutibacterium acnes<, bioRxiv, pp.2023, ~
- Jeong, J.-W., "Ethanol extract of Poria cocos reduces the production of inflammatory mediators by suppressing the NF-kappaB signaling pathway in lipopolysaccharide-stimulated RAW 264. 7macrophages", BMC Complementary and Alternative Medicine, 14, pp.1~8 (2014)
- Kadam, S. U., "Application of Novel Extraction Technologies for Bioactives from Marine Algae", Journal of Agricultural and Food Chemistry, 61(20), pp.4667~4675 (2013)
- Kasimala, M. B., "A review on biochemical composition and nutritional aspects of seaweeds", Caribbean Journal of Sciences and Technology(CJST), 3(1), pp.789~797 (2015)
- Kılınç, B., "Seaweeds for food and industrial applications. In Food industry" (2013), IntechOpen
- Kim, S.-N., "Anti-diabetic and hypolipidemic effects of Sargassum yezoense in db", db mice, 2012, pp.424(4), 675~680
- Kwon, Y.-J., "Chamaecyparis obtusa (Siebold & Zucc.) Endl. leaf extracts prevent inflammatory responses via inhibition of the JAK", STAT axis in RAW264.7 cells, 2022, pp.282, 114493~
- Lee, H.-S., "Anti-inflammatory effects of Allium cepa L. peel extracts via inhibition of JAK-STAT pathway in LPS-stimulated RAW264.7 cells", Journal of Ethnopharmacology, 317, pp.116851~ (2023)[DOI]
- Li, Y., "Brown algae carbohydrates : Structures, pharmaceutical properties, and research challenges", Marine Drugs, 19(11), pp.620~ (2021)
- Liu, C., "Autophagy in skin barrier and immune-related skin diseases", The Journal of Dermatology, 48(12), pp.1827~1837 (2021)
- Megha, K., "Cascade of immune mechanism and consequences of inflammatory disorders", Phytomedicine, 91, pp.153712~ (2021)
- Meyer-Hoffert, U., "Reddish, scaly, and itchy : how proteases and their inhibitors contribute to inflammatory skin diseases", Archivum immunologiae et therapiae experimentalis, 57, pp.345~354 (2009)
- Mikołajczyk, T., "A new generation of fibers from alginic acid for dressing materials", Journal of applied polymer science, 107(3), pp.1670~1677 (2008)
- Muszer, M., "Human Microbiome : When a Friend Becomes an Enemy", Archivum immunologiae et therapiae experimentalis, 63(4), pp.287~298 (2015)
- Nørreslet, L. B., "The skin microbiome in inflammatory skin diseases", Current Dermatology Reports, 9, pp.141~151 (2020)
- Ring, J., "Atopic eczema : role of microorganisms on the skin surface", Allergy, 47(4), pp.265~269 (1992)
- K. K. Asanka Sanjeewa, "Ethanol extract separated from Sargassum horneri (Turner) abate LPS-induced inflammation in RAW 264.7 macrophages", Fisheries and Aquatic Sciences, 22(1), pp.1~10 (2019), 한국수산과학회[DOI]
- Sawyer, D. T., "How super is superoxide", Accounts of Chemical Research, 14(12), pp.393~400 (1981)
- Serafini, M., "Flavonoids as anti-inflammatory agents", Proceedings of the Nutrition Society, 69(3), pp.273~278 (2010)
- Sun, K.-Y., "Lactobacillus paracasei modulates LPS-induced inflammatory cytokine release by monocyte-macrophages via the up-regulation of negative regulators of NF-kappaB signaling in a TLR2-dependent manner", Cytokine, 92, pp.1~11 (2017)
- Urban, K., "Burden of skin disease and associated socioeconomic status in Asia : A cross-sectional analysis from the Global Burden of Disease Study 1990-2017", JAAD International, 2, pp.40~50 (2021)
- Velu, G., "Phytochemical and pharmacological importance of plant secondary metabolites in modern medicine", Bioorganic phase in natural food: an overview, pp.135~156 (2018)
- Wang, L., "The potential of sulfated polysaccharides isolated from the brown seaweed Ecklonia maxima in cosmetics : Antioxidant, anti-melanogenesis, and photoprotective activities", Antioxidants, 9(8), pp.724~ (2020)
- Yahfoufi, N., "The immunomodulatory and anti-inflammatory role of polyphenols", Nutrients, 10(11), pp.1618~ (2018)
- Yan, Z., "Role of the JAK", STAT signaling pathway in regulation of innate immunity in neuroinflammatory diseases, 2018, pp.189, 4~13
- Zhang, T., "NF-κB signaling in inflammation and cancer", MedComm, 2(4), pp.618~653 (2021)