Ethanol extract separated from Sargassum horneri (Turner) abate LPS-induced inflammation in RAW 264.7 macrophages
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Background: This study is aimed at identifying the anti-inflammatory properties of 70% ethanol extract produced from an edible brown seaweed Sargassum horneri (SJB-SHE) with industrial-scale production by Seojin Biotech Co. Ltd. S. horneri is a rich source of nutrient and abundantly growing along the shores of Jeju, South Korea. Methods: Here, we investigated the effect of SJB-SHE on LPS-activated RAW 264.7 macrophages. The cytotoxicity and NO production of SJB-SHE were evaluated using MTT and Griess assays, respectively. Additionally, protein expression and gene expression levels were quantified using ELISA, Western blots, and RT-qPCR. Results: Our results indicated that pre-treatment of RAW 264.7 macrophages with SJB-SHE significantly inhibited LPS-induced NO and PGE2 production. SJB-SHE downregulated the proteins and genes expression of LPS-induced iNOS and COX2. Additionally, SJB-SHE downregulated LPS-induced production of pro-inflammatory cytokines (tumor necrosis factor-α, interleukin (IL)-6, and IL-1β). Furthermore, SJB-SHE inhibited nuclear factor kappa-B (NF-κB) activation and translocation to the nucleus. SJB-SHE also suppressed the phosphorylation of mitogen-activated protein kinases (ERK1/2 and JNK). Conclusions: Collectively, our results demonstrated that SJB-SHE has a potential anti-inflammatory property to use as a functional food ingredient in the future.
Abstract
Background: This study is aimed at identifying the anti-inflammatory properties of 70% ethanol extract produced from an edible brown seaweed Sargassum horneri (SJB-SHE) with industrial-scale production by Seojin Biotech Co. Ltd. S. horneri is a rich source of nutrient and abundantly growing along the shores of Jeju, South Korea. Methods: Here, we investigated the effect of SJB-SHE on LPS-activated RAW 264.7 macrophages. The cytotoxicity and NO production of SJB-SHE were evaluated using MTT and Griess assays, respectively. Additionally, protein expression and gene expression levels were quantified using ELISA, Western blots, and RT-qPCR. Results: Our results indicated that pre-treatment of RAW 264.7 macrophages with SJB-SHE significantly inhibited LPS-induced NO and PGE2 production. SJB-SHE downregulated the proteins and genes expression of LPS-induced iNOS and COX2. Additionally, SJB-SHE downregulated LPS-induced production of pro-inflammatory cytokines (tumor necrosis factor-α, interleukin (IL)-6, and IL-1β). Furthermore, SJB-SHE inhibited nuclear factor kappa-B (NF-κB) activation and translocation to the nucleus. SJB-SHE also suppressed the phosphorylation of mitogen-activated protein kinases (ERK1/2 and JNK). Conclusions: Collectively, our results demonstrated that SJB-SHE has a potential anti-inflammatory property to use as a functional food ingredient in the future.
참고문헌 (26)
- Akira S., "Advances in immunology, vol 78", pp.1~56 (2001), Academic Press
- da Silva RPFF, "Supercritical fluid extraction of bioactive compounds", TrAC Trends Anal Chem, 76, pp.40~51 (2016)
- Fleurence J, "Seaweed proteins : biochemical, nutritional aspects and potential uses", Trends Food Sci Technol, 10, pp.25~28 (1999)
- Herath K, "Differential modulation of immune response and cytokine profiles of Sargassum horneri ethanol extract in murine spleen with or without Concanavalin A stimulation", Biomed Pharmacother, 110, pp.930~942 (2019)
- Hseu YC, "Antiinflammatory potential of Antrodia Camphorata through inhibition of iNOS, COX-2 and cytokines via the NF-kappaB pathway", Int Immunopharmacol, 5, pp.1914~1925 (2005)
- Huang C, "Molecular analysis of Sargassum from the northern China seas", Phytotaxa, 319, pp.71~83 (2017)
- Jayawardena TU, "Sargassum horneri (Turner) C. Agardh ethanol extract inhibits the fine dust inflammation response via activating Nrf2", HO-1 signaling in RAW 264.7 cells, 2018, pp.18, 249~
- Jung HA, "Anti-inflammatory activity of edible brown alga Eisenia bicyclis and its constituents fucosterol and phlorotannins in LPSstimulated RAW264.7 macrophages", Food Chem Toxicol, 59, pp.199~206 (2013)
- Kadam SU, "Marine foods as functional ingredients in bakery and pasta products", Food Res Int, 43, pp.1975~1980 (2010)
- Kiemer AK, "Phyllanthus amarus has anti-inflammatory potential by inhibition of iNOS, COX-2, and cytokines via the NF-κB pathway", J Hepatol, 38, pp.289~297 (2003)
- Hyun-Soo Kim, "A comparative study of Sargassum horneri Korea and China strains collected along the coast of Jeju Island South Korea: its components and bioactive properties", ALGAE, 33(4), pp.341~349 (2018), 한국조류학회I[DOI]
- Kim MM, "Anti-inflammatory effect of Ishige okamurae ethanolic extract via inhibition of NF-kappaB transcription factor in RAW 264. 7 cells", Phytother Res, 23, pp.628~634 (2009)
- Kubo N, "Development and characterization of simple sequence repeat markers for genetic analyses of Sargassum horneri(Sargassaceae, Phaeophyta)populations in Kyoto, Japan", J Appl Phycol, 29, pp.1729~1733 (2017)
- Li KK, "Dihydrofisetin exerts its anti-inflammatory effects associated with suppressing ERK", p38 MAPK and Heme Oxygenase-1 activation in lipopolysaccharide-stimulated RAW 264.7 macrophages and carrageenaninduced mice paw edema, 2018, pp.54, 366~374
- Lund AK., "Comprehensive toxicology", pp.243~274 (2010), Elsevier
- Ma AC, "Isolation of the molecular species of monogalactosyldiacylglycerols from brown edible seaweed Sargassum horneri and their inhibitory effects on triglyceride accumulation in 3T3-L1 adipocytes", J Agric Food Chem, 62, pp.11157~11162 (2014)
- Merchant M, "Crocodylian nuclear factor kappa B", Comp Biochem Physiol B Biochem Mol Biol, 213, pp.28~34 (2017)
- Mulgund A, "Handbook of fertility", pp.273~281 (2015), Academic Press
- Nomura M, "Seasonal variations of total lipids, fatty acid composition, and fucoxanthin contents of Sargassum horneri(Turner)and Cystoseira hakodatensis(Yendo)from the northern seashore of Japan", J Appl Phycol, 25, pp.1159~1169 (2012)
- Pugh TJ, "Clinical radiation oncology", pp.83~94 (2012), W.B. Saunders
- Salter DM., "Rheumatology", pp.61~64 (2015), Content Repository Only
- KALU KAPUGE ASANKA SANJEEWA, "Anti-inflammatory activity of a sulfated polysaccharide isolated from an enzymatic digest of brown seaweed Sargassum horneri in RAW 264.7 cells", Nutrition Research and Practice, 11(1), pp.3~10 (2017), 한국영양학회
- Surh Y-J, "Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-κB activation", Mutat Res Fundam Mol Mech Mutagen, 480-481, pp.243~268 (2001)
- Wijesinghe W, "Biological activities and potential cosmeceutical applications of bioactive components from brown seaweeds : a review", Phytochem Rev, 10, pp.431~443 (2011)
- Xie X, "Development of oogonia of Sargassum horneri(Fucales, Heterokontophyta)and concomitant variations in PSII photosynthetic activities", Phycologia, 53, pp.10~14 (2014)
- Zielinski MR, "Therapy in sleep medicine", pp.607~616 (2012), W.B. Saunders