피부재생용 나노셀룰로스가 포함된 복합 소재의 제조 및 특성평가
Manufacturing and characterization of composite materials containing nanocellulose for skin regeneration
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초록
본 연구에서는 우뭇가사리 나노셀룰로스, 새싹삼 추출물, 담팔수 추출물이 포함된 복합소재의 제조 및 항균, 항염, 창상치유 효과를 연구하여 드레싱제의 원료로 활용하고자 한다. 이를 위해 나노셀룰로스 제조 검증, HPLC 분석을 통한 compound K와 Pentagalloylglucose 함량분석, 복합추출물 배합비에 따른 항균 활성 평가, NO 생성량을 통한 항염효능, SD rat을 통한 창상치유 검증을 수행하였다. 실험결과 각각의 추출물에 항균, 항염효능을 가지는 Compound K (0.38 ± 0.02 mg/mL) , PGG (177.67 μg/mL)가 포함된 정도와 SS3DP7에서 표피포도상구균과 황색포도상구균에 대한 항균 효능을 확인하였다. 복합소재는 55% 이상의 항염증 효과와 창상 유발 후 1~2일 차에 대조군 대비 10% 이상 높은 창상회복율이 관찰되었다. 추후 위와 같은 친환경 소재의 효능을 활용한 습윤드레싱 제품 개발을 진행하고자 한다.
Abstract
In this study, we will study the antibacterial, anti-inflammatory, and wound healing effects of a composite material containing Gelidium amansii nanocellulose, ginseng extract, and Elaeocarpus sylvestris var. elliptcus extract and use it as a raw material for a dressing. To this end, we performed verification of nanocellulose manufacturing, analysis of compound K and pentagalloylglucose contents through HPLC analysis, evaluation of antibacterial activity according to the mixing ratio of the composite extract, anti-inflammatory efficacy through NO production, and wound healing verification using SD rats. As a result of the experiment, the degree of Compound K (0.38 ± 0.02 mg/mL) and PGG (177.67 μg/mL) containing antibacterial and anti-inflammatory efficacy in each extract was confirmed, and antibacterial efficacy against Staphylococcus epidermidis and Staphylococcus aureus was confirmed in SS3DP7. The composite material showed an anti-inflammatory effect of more than 55%, and a wound healing rate higher than 10% was observed compared to the control group 1-2 days after wound induction. In the future, we plan to develop a wet dressing product that utilizes the efficacy of eco-friendly materials as described above.
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