Effectiveness of Ginseng Berry Marc Extract for Skin Improvement

Abstract

Purpose: This study identifies the possibility of ginseng berry marc as a cosmetic material for skin improvement through a physiological activation experiment. Methods: The measurement of the total polyphenol and flavonoid content, evaluation of antioxidants through DPPH radical scavenging activity, and cell viability ratio of 70% ethanol extract of ginseng berry marc extracts were conducted. To access the anti-inflammatory effects, NO generative inhibitory measurements and cell regeneration by cell migration assessment were investigated. Results: The total polyphenol content was 335±12.3 mg/g, and the total flavonoid content was 79±3.2 mg/g. DPPH radical scavenging activity also showed a concentration￾dependent increase as the concentration increased to 37.73%±2.47% at 0.25 mg/ mL, 49.33%±0.66% at 0.5 mg/mL, 73.56%±3.56% at 0.75 mg/mL, and 80.51%± 0.51% at 0.9 mg/mL. The cell viability evaluation (MTT assay) was 93.33%±1.88% at 0.25 mg/mL, 90.66%±1.24% at 0.5 mg/mL, 88.66%±1.69% at 0.75 mg/mL, and 83.1%±0.94% at 0.9 mg/mL. At concentrations of 0.25 and 0.5 mg/mL, the cell survival rate is greater than 90%, and the lowest survival rate is greater than 80%, indicating that there is no problem with the safety of cytotoxicity. The anti￾inflammatory evaluation showed that NO decreased as the concentration increased with 31.76±0.52 μM at 0.25 mg/mL, 21.76±0.52 μM at 0.5 mg/mL, 10.86± 0.47 μM at 0.75 mg/mL, and 9.76±0.28 μM at 0.9 mg/mL. According to the comparison of cell movement and recovery conditions by concentration, the higher the concentration 2.5 mg/mL, 5 mg/mL, and 10 mg/mL, the faster space is filled, which can help the skin recover and regenerate. Conclusion: Eco-friendly natural ingredients can be obtained through upcycling of ginseng berry marc, which can be used as an effective skin-improving cosmetics ingredient through more studies.