Streptomyces shinuiensis sp. nov., a Salternamides-Producing Bacterium Isolated from Saltern Sediment
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Strain HK10T , previously isolated from saltern sediment and identified as a salternamides-producing bacterium, was taxonomically characterized in the present study. The strain is a Gram-stain-positive, aerobic, and filamentous actinobacterium. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HK10T belongs to the genus Streptomyces and is most closely related to Streptomyces radiopugnans R97T (99.4% sequence similarity), followed by other Streptomyces species with sequence similarities of ≤98.9%. The draft genome sequence of strain HK10T was 6.69 Mbp and a DNA G+C content of 72.7%. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain HK10T and type strains of the genus Streptomyces were ≤92.7% and ≤53.0%, respectively, which are below the thresholds for species delineation, indicating that strain HK10T represents a novel species. The major cellular fatty acids were iso-C16:0, anteisoC15:0, anteiso-C17:0, and iso-C14:0. The predominant menaquinone was menaquinone-9 (MK-9). The polar lipid profile included phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol dimannoside. Whole-cell hydrolysates of strain HK10T contained L,L-diaminopimelic acid and glycine. Based on phylogenetic, genomic, chemotaxonomic, and physiological evidence, strain HK10T is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces shinuiensis sp. nov. is proposed. The type of strain is HK10T (= NBRC 114904T = KACC 22137T ).
Abstract
Strain HK10T , previously isolated from saltern sediment and identified as a salternamides-producing bacterium, was taxonomically characterized in the present study. The strain is a Gram-stain-positive, aerobic, and filamentous actinobacterium. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HK10T belongs to the genus Streptomyces and is most closely related to Streptomyces radiopugnans R97T (99.4% sequence similarity), followed by other Streptomyces species with sequence similarities of ≤98.9%. The draft genome sequence of strain HK10T was 6.69 Mbp and a DNA G+C content of 72.7%. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain HK10T and type strains of the genus Streptomyces were ≤92.7% and ≤53.0%, respectively, which are below the thresholds for species delineation, indicating that strain HK10T represents a novel species. The major cellular fatty acids were iso-C16:0, anteisoC15:0, anteiso-C17:0, and iso-C14:0. The predominant menaquinone was menaquinone-9 (MK-9). The polar lipid profile included phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol dimannoside. Whole-cell hydrolysates of strain HK10T contained L,L-diaminopimelic acid and glycine. Based on phylogenetic, genomic, chemotaxonomic, and physiological evidence, strain HK10T is considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces shinuiensis sp. nov. is proposed. The type of strain is HK10T (= NBRC 114904T = KACC 22137T ).