벼 재조합집단을 이용한 Low Glutelin 계통의 품질분석 및 유망계통 육성
Analysis of Eating Quality in Recombinant Inbred Lines and Selection of Elite Line with Low Glutelin Content in Rice
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초록
In chronic renal failure, to reduce the burden on the kidneys, it is necessary to provide patients with therapy based on a low-protein diet. This study was conducted to improve eating quality for developing low easy to-digest protein rice variety with Lgc1 gene. Two hundred twenty six recombinant inbred lines (RILs) were used, which were derived from the cross between Chugoku173, a low glutelin rice variety developed in Japan, and Junambyeo, a high quality rice variety in Korea. We investigated glutelin phenotype using SDS PAGE and its genotype by functional DNA marker. As results of them were consistent, this DNA marker is expected to use of MAS for the Lgc1- mediated low glutelin variety. The eating quality of rice measured by Toyo meter (GMT) showed significantly negative correlation with amylose content, but there was no significance with protein content in this population. Dividing the RILs into normal amylose group (app. 19%) and low amylose group (app. 13%), the eating quality estimated by GMT was generally lower in low glutelin lines, but that of low amylose group was higher than that of normal amylose group. On the basis of several results, it is supposed that low amlyose gene can be used to breed the low glutelin rice variety with high eating quality. Through the analysis of agronomic traits related to eating quality, Milyang262, low glutelin and amylose rice variety, were developed.
Abstract
In chronic renal failure, to reduce the burden on the kidneys, it is necessary to provide patients with therapy based on a low-protein diet. This study was conducted to improve eating quality for developing low easy to-digest protein rice variety with Lgc1 gene. Two hundred twenty six recombinant inbred lines (RILs) were used, which were derived from the cross between Chugoku173, a low glutelin rice variety developed in Japan, and Junambyeo, a high quality rice variety in Korea. We investigated glutelin phenotype using SDS PAGE and its genotype by functional DNA marker. As results of them were consistent, this DNA marker is expected to use of MAS for the Lgc1- mediated low glutelin variety. The eating quality of rice measured by Toyo meter (GMT) showed significantly negative correlation with amylose content, but there was no significance with protein content in this population. Dividing the RILs into normal amylose group (app. 19%) and low amylose group (app. 13%), the eating quality estimated by GMT was generally lower in low glutelin lines, but that of low amylose group was higher than that of normal amylose group. On the basis of several results, it is supposed that low amlyose gene can be used to breed the low glutelin rice variety with high eating quality. Through the analysis of agronomic traits related to eating quality, Milyang262, low glutelin and amylose rice variety, were developed.
참고문헌 (13)
- Bousse L, "Protein sizing on a microchip", Anal. Chem, 73, pp.1207~1212 (2001)
- Iida S, "A rice (Oryza sativa L.) mutant having a low content of glutelin and a high content of prolamine", Theor. Appl. Genet, 87, pp.374~378 (1993)
- Juliano, B. O., "A simplified assay for milled-rice amylose", Cereal Sci. Today, 16, pp.334~336 (1971)
- Kusaba M, "Low glutelin content 1: a dominant mutation that suppresses the glutelin multigene family via RNA silencing in rice", The Plant Cell, 15, pp.1455~1467 (2003)
- Mochizuki T, "Usefulness of low protein rice in diet therapy in patients with chronic renal failure", Jpn. J. Nephrol, 42, pp.24~29 (2000)
- Morita R, "Development of PCR markers to detect the glb1 and Lgc1 mutations for the production of low easy-todigest protein rice varieties", Theor. Appl. Genet, 119, pp.125~130 (2009)
- Nishimura M, "New rice varieties with low levels of easy to digest protein, LGC-Katu and LGC-Jun", Kor. J. Breed. Sci, 55, pp.103~105 (2005)
- Ogawa M, "Purification of protein body- I of rice seed and its polypeptide composition", Plant Cell Physiol, 28, pp.1517~1528 (1987)
- Sato H, "Selection for high palatable rice lines by use of mido meter and rapid visco analyzer", Jpn. J. Crop. Sci, 72, pp.390~394 (2003)
- Son JR, "Trend and future research of rice quality evaluation", Korean J. Crop Sci, 47, pp.33~54 (2002)
- Tanaka K, "Genetic analysis of rice storage proteins", Proc Inst Rice Genetic Symp. Int Rice Res Inst, pp.887~897 (1986)
- Tanaka K, "Isolation and characterization of two types of protein bodises in the rice endosperm", Agric. Biol. Chem, 44, pp.1633~1639 (1980)
- Youichi O, "Distribution of seed proteins in rice grain of seed-protein mutant cultivars", Jpn. J. Crop Sci, 78, pp.58~65 (2009)