N-methyl-N-nitro-N-nitrosoguanidine 노출에 따른 바지락 (Ruditapes philippinarum) 상피조직의 병리조직학적 반응
Histopathological changes of epithelium following the exposure of N-methyl-N-nitro-N-nitrosoguanidine in Manila clam, Ruditapes philippinarum
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이매패류의 각종 화학적 유해인자에 대한 반응특성을 이해하기 위한 연구의 일환으로 화학적 오염물질의 하나인 N-methyl-N-nitro-N-nitrosoguanidine (MNNG)을 바지락에 개체당 1.0 mg씩 단회 주사한 후 240시간 동안 조직손상의 과정을 경시적으로 추적하였다. MNNG에 의하여 조직변화를 보인 곳은 위, 중장 및 후장, 소화맹낭, 생식소로 한정되어 있었다. MNNG를 주사한 후 가장 먼저 변성변화를 보인 조직은 중장상피였으며, 주사 후 12시간째부터 조직학적 변화를 인정할 수 있었다. 소화관 상피 내에는 혈구 (hemocyte)를 포함한 단핵성 괴사세포가 상피에 대량으로 침윤하는 동시에 부분적이거나 완전한 상피파괴 소견이 실험종료까지 확인되었다. 가장 마지막으로 조직학적 변화를 보인 장기는 생식소였으며, 주사 후 144시간째부터 생식상피의 괴사성 탈락을 인정할 수 있었다. 또한, 혈구에 특이적으로 결합하는 Ricinus communis (RCA-Ⅰ) lectin으로 조직화학염색을 실시한 결과에서 이들 소화상피에 침윤하는 세포가 대부분 혈구임이 강하게 시사되었다. 이상의 결과에서, 소화기계가 MNNG의 비경구적 노출에 의해 손상받기 쉬우며, MNNG에 의해 야기되는 소화상피 파괴에는 혈구가 깊이 관여하는 것으로 사료되었다.
Abstract
To understand responses of bivalve tissues to various injurious agents, histopathological observation was done in the Manila clam, Ruditapes philippinarum, during 240 hours after an intramuscular injection of N-methyl-N-nitro-N-nitrosoguanidine (MNNG). Some histopathological changes were observed with the epithelia of stomach, midgut, hindgut, digestive diverticula and gonads after injecting MNNG. The earliest degenerative changes were recognized in the epithelium of midgut 12 hours after the injection. Partial or complete, epithelial destruction was constantly accompanied by the massive infiltration of a mononuclear, necrotic cell group including hemocytes. At 144 hours later, gonads showed the necrotic desquamation of their germinal epithelia. Nearly all of the infiltrated cells within the destructive epithelium were suggested to be hemocytes, from the binding property with a hemocyte-specific lectin, Ricinus communis (RCA-1). From these results it was concluded that the epithelium of digestive system is vulnerable to parenteral MNNG and hemocytes might be deeply involved in this MNNG-induced destruction of the digestive epithelium.
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