대복, Gomphina veneriformis 아가미의 조직학적 변화와 산소소비율에 미치는 TBTCl의 독성
Tributyltin chloride (TBTCl) toxicity on the oxygen consumption rate and histological changes of gill in the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae)
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
This study was conducted to find out biological response of bivalves exposed to tributyltin chloride (TBTCl). The results of the study confirmed that TBTCl induce the reduction of oxygen consumption rate and histopathological feature in the gill structure of equilateral venus, Gomphina veneriformis. The experimental groups consisted of a control and 3 TBTCl exposure groups (0.4 ㎛ TBTCl L-¹, 0.6 ㎛ TBTCl L-¹, 0.8 ㎛ TBTCl L-¹) and the experimental period was 36 weeks. For histological analysis, gill tissues were fixed in Bouin's fluid and then stained H-E stain, AB-PAS (pH 2.5) reaction and Masson's trichrome stain after having serially sectioned the tissue by paraffin method at thickness of 4~6 ㎛. The oxygen consumption rate was not significantly different between the control and exposure groups at 4 weeks, but in all exposure groups at 28 weeks, it was significantly different to the control. Gill of G. veneriformis had demibranch that attached two sheets of lamellae and a lamella was composed of numerous filaments, numbering 25 on average. The frontal filament zone had three types of cilia; frontal, latero-frontal and lateral depending on locations while the lateral cilia were the longest and largest in number. The mucous cells observed in filaments were more abundant in the frontal filament zone than abfrontal filament zone and mucous substances were showed blue color (542c) in AB-PAS (pH 2.5) reaction. Gill exposed to TBTCl was extended hemolymph sinus and increased hemocytes at 4 weeks, and then it showed increases of mucous cells and partially disappearance of frontal cilia. In the group of 0.8 ㎍ TBTCl L-¹ at 12 weeks, hypertrophy of frontal and latero-frontal epithelia was observed. Also it observed m decrease of mucous cell containing weekly acid mucosubstance and appeared partially destruction muscle fiber bundle. In the groups of 0.4 and 0.6 ㎍ TBTCl L-¹ at 36 weeks, it appeared partially modification of epithelia and in 0.8 ㎍ TBTCl L-¹ group, observed filaments that come out chitinous rod from disappearance of frontal and latero-frontal epithelia.
Abstract
This study was conducted to find out biological response of bivalves exposed to tributyltin chloride (TBTCl). The results of the study confirmed that TBTCl induce the reduction of oxygen consumption rate and histopathological feature in the gill structure of equilateral venus, Gomphina veneriformis. The experimental groups consisted of a control and 3 TBTCl exposure groups (0.4 ㎛ TBTCl L-¹, 0.6 ㎛ TBTCl L-¹, 0.8 ㎛ TBTCl L-¹) and the experimental period was 36 weeks. For histological analysis, gill tissues were fixed in Bouin's fluid and then stained H-E stain, AB-PAS (pH 2.5) reaction and Masson's trichrome stain after having serially sectioned the tissue by paraffin method at thickness of 4~6 ㎛. The oxygen consumption rate was not significantly different between the control and exposure groups at 4 weeks, but in all exposure groups at 28 weeks, it was significantly different to the control. Gill of G. veneriformis had demibranch that attached two sheets of lamellae and a lamella was composed of numerous filaments, numbering 25 on average. The frontal filament zone had three types of cilia; frontal, latero-frontal and lateral depending on locations while the lateral cilia were the longest and largest in number. The mucous cells observed in filaments were more abundant in the frontal filament zone than abfrontal filament zone and mucous substances were showed blue color (542c) in AB-PAS (pH 2.5) reaction. Gill exposed to TBTCl was extended hemolymph sinus and increased hemocytes at 4 weeks, and then it showed increases of mucous cells and partially disappearance of frontal cilia. In the group of 0.8 ㎍ TBTCl L-¹ at 12 weeks, hypertrophy of frontal and latero-frontal epithelia was observed. Also it observed m decrease of mucous cell containing weekly acid mucosubstance and appeared partially destruction muscle fiber bundle. In the groups of 0.4 and 0.6 ㎍ TBTCl L-¹ at 36 weeks, it appeared partially modification of epithelia and in 0.8 ㎍ TBTCl L-¹ group, observed filaments that come out chitinous rod from disappearance of frontal and latero-frontal epithelia.
참고문헌 (49)
- Abel, P.D., "Effects of some pollutants on the filtration rate of Mytilus edulis", Mar. Poll. Bull, 7, pp.228~231 (1976)
- Allen, J.A., "Preliminary experiments on the feeding and excretion of bivalves using Phaeodactylum labelled with 32P", J. Mar. Biol. Ass. U.K, 42, pp.609~623 (1962)
- Asmus, R.M., "Phytoplankton-mussel bed interactions in intertidal ecosystems, In: Bivalve filter feeders in estuarine and coastal ecosystem processes NATO, A.S.I. Series", pp.57~84 (1993), Springer-Verlag
- Beninger, P.G., "New observations of the gills of Placopecten magellanicus (Mollusca: Bivalvia), and implications for nutrition", Mar. Biol, 98, pp.61~70 (1998)
- Bigas, M., "Cytological effects of experimental exposure to Hg on the chilled epithelium of the European flat oyster Ostrea edulis: ultrastructural and quantitative changes related to bioaccumulation", Tissue Cell, 33, pp.178~188 (2001)
- Cranford, P.J., "Particle clearance and absorption of phytoplankton and detritus by the sea scallop Placopecten magellanicus (Gmelin)", J. Exp. Mar. Biol. Ecol, 137, pp.105~121 (1990)
- David, J.A.O., "Fine structure of Mytella falcata (Bivalvia) gill filaments. Micron" (2007), Article in Press, Corrected Proof
- Delhaye, W., "Contribution to the study of the effect of copper on Mytilus edulis during reproductive period", Comp. Biochem. Physiol, 50, pp.511~518 (1975)
- Drury, R.A.B., "Carleton's histological technique", pp.520~ (1980), Oxford University Press
- Dubey, S.K., "Biodegradation of tributyltins (organotins) by marine bacteria", Appl. Organometal Chem, 17, pp.3~8 (2003)
- Eble, A.F., "Biology of the hard clam. In: Anatomy and histology of Mercenaria mercenaria", pp.117~220 (2001), Elsevier
- Foster-Smith, R.L., "The effect of concentration of suspension on the filtration rates and pseudofaecal production on Mytilus edulis (L.), Cerastoderma edule (L.) and Venerupis pullastra (Montagu)", J. Exp. Mar. Biol. Ecol, 17, pp.1~22 (1975)
- Gauthier-Clerc, S., "Delayed gametogenesis of Mya arenaria in the Saguenay fjord (Canada): a consequence of endocrine disruptors?", Comp. Biochem. Physiol, 131, pp.457~467 (2002)
- Gerdes, D., "The Pacific Oyster Crassostrea gigas Part Ⅰ. Feeding behaviour of larvae and adults", Aquaculture, 31, pp.195~219 (1983)
- Gómez-Mendikute, A., "Characterization of mussel gill cells in vivo and in vitro", Cell Tissue Res, 321, pp.131~140 (2005)
- Gregory, M.A., "The effect of mercury exposure on the surface morphology of gill filaments in Perna perna (Mollusca: Bivalvia)", Mar. Pollut. Bull, 39, pp.116~121 (1999)
- Griffiths, R.J., "Filtration, oxygen consumption and assimilation in the black mussel Choromytilus meridionalis", Mar. Ecol. Prog. Ser, 3, pp.63~70 (1980)
- Hinch, S.G., "Size-and age-specific patterns of trace metal concentrations on fresh water clams from on acid-sensitive and a circumneutral lake", J. Zool, 65, pp.2436~2442 (1987)
- Kim S.Y., "The effects of pollutants effluent from a steam-power plant on coastal bivalves", Ocean. Res, 10(1), pp.47~56 (1988)
- Laughlin, R.B., "Tributyltin in seawater: speciation and octanol-water partition coefficient", Environ. Sci. Technol, 20, pp.201~204 (1986)
- Manley, A.R., "The effects of cooper on the behaviour, respiration, filtration and ventilation activity of Mytilius edulis", J. Mar. Biolog. Assoc. U.K, 63, pp.205~222 (1984)
- Matozzo, V., "Evaluation of 4-nonylphenol toxicity in the clam Tapes phhilippinarum", Environ. Res, 91, pp.179~185 (2003)
- Morrison, C.M., "Histology and cell ultrastructure of the mantle and mantle lobes of the Estern oyster, Crassostrea virginica Gmelin: A summary atlas", Amer. Malac. Bull, 10(1), pp.1~24 (1993)
- Morse, M.P., "Bivalvia. In: Microscopic anatomy of invertebrates", pp.7~118 (1997), A John Wiley & Sons, Inc., Publication
- Morton, B.S., "The biology and functional morphology of the weisted ark Trisidos semitorta (Bicalvia: Arcacea) with a discussion on shell "torsion" in the genus", Malacologia, 23, pp.375~396 (1983)
- Morton, B.S., "The biology, ecology and functional aspects of the organs of feeding and digestion of the South East Asian mangrove bivalve, Enigmonia aenigmatica (Mollusca: Anomiacea)", J. Zool. Lond, 179, pp.437~466 (1976)
- Naimo, T.J., "Sublethal effects of cadmium on physiological responses in the pocketbook mussel, Lampsilis ventricosa", Environ. Toxicol. Chem, 11, pp.1013~1021 (1992)
- Nelson, T.C., "The feeding mechanism of the oyster. II. On the gills and palps of Ostrea edulis, Crassostrea viginica and Crassostrea angulata", J. Morphol, 107, pp.163~191 (1960)
- Otludil, B., "The effects of endosulfan on the great ramshorn snail, Planorbariua corneus (Gastropoda, Pulmonata): a histopathological study", Chemosphere, 56, pp.707~716 (2004)
- Palmer, R.E., "Behavioral and rhythmic aspects of filtration in the bay scallop, Argopecten irradians concentricus (Say), and the oyster, Crassostrea virginica (Gmelin)", J. Exp. Mar. Biol. Ecol, 45, pp.273~295 (1980)
- Park, J.J., "The effect of long-term cadmium exposure on the organ structure of the equilateral venus, Gomphina veneriformis (Bivalvia: Veneridae)", Bull. Fish. Sci. Inst. Chonnam Nat'l Univ, 15, pp.10~12 (2006)
- Pekkarinen, M., "Scanning electron microscopy, whole-mount histology, and histochemistry of two Anodontine glochidia (Bivalvia: Unionidae)", J. Zool, 74, pp.1964~1973 (1996)
- Quinn, B., "The endocrine disrupting effect of municipal effluent on the zebra mussel (Dreissena polymorpha)", Aquat. Toxicol, 66, pp.297~292 (2004)
- Rasmussen, L.P.D., "Light and electron microscopic studies of the acute and chronic toxic effects of N-nitoroso compounds on the marine mussel, Mytilus edulis (L). Ⅱ. N-methyl-N-nitro-N- nitrodoguanidine", Aquat. Toxicol, 3, pp.301~311 (1983)
- Regoli, F., "Accumulation and subcelluar distribution of metals (Cu, Fe, Mn, Pb and Zn) in the Mediterranean mussel, Mytilus galloprovincialis during a field transplant experiment", Mar. Pollut. Bull, 28, pp.592~600 (1994)
- Riisgård, H.U., "Efficiency of particle retention and filtration rate in 6 species of Northeast American bivalves", Mar. Ecol. Prog. Ser, 45, pp.217~223 (1988)
- Schulte, E.H., "Influence of algal concentration and temperature on the filtration rate of Mytilus edulis", Mar. Biol, 30, pp.331~341 (1975)
- Shumway, S.E., "Particle selection, ingestion, and absorption in filter-feeding bivalves", J. Exp. Mar. Biol. Ecol, 91, pp.77~92 (1985)
- Silvester, N.R., "Hydrodynamic aspect of particle capture by Mytilus", J. Mar. Biol. Assoc. U.K, 64, pp.859~879 (1984)
- Sobral, P., "Effects of copper exposure on the scope for growth of the clam Ruditapes decussatus from southern Portugal", Mar. Pollut. Bull, 34, pp.992~1000 (1997)
- Sunila, I., "Survival, growth and shell deformities of copper-and cadmium-exposed mussels, Mytilus edulis L. in Brackish water. Estuar", Coast. Shellf Sci, 21, pp.555~565 (1985)
- Sunila, I., "Acute histopathological responses of the gill of the mussels, Mytilus edulis, to exposure by environmental pollutants", J. Invert. Pathol, 52, pp.137~141 (1988)
- Sunila, I., "Chronic histopathological effects of short-term copper and cadmium exposure on the gill of the mussels, Mytilus edulis", J. Invert. Pathol, 47, pp.125~142 (1986)
- Sze, P.W.C., "Effects of chronic copper exposure on the green mussel Perna viridis", Mar. Biol, 137, pp.379~392 (2000)
- U.S. E.P.A., "Abmbient aquatic life water quality criteria for tributyltin (TBT)-final", United States Environmental Protection Agency, pp.129~ (2003)
- Watling H.R., "Comparative effects of metals on the filtering rate of brown mussel, Perna perna", Bull. Environ. Contam. Toxicol, 29, pp.651~657 (1982)
- Widdows, J., "Effects of tributyltin and dibutyltin on the physiological energetica of the mussel, Mytilus edulis", Mar. Environ. Res, 35, pp.233~249 (1993)
- Winter, J.E., "A review on the knowledge of suspension-feeding in lamellibranchiate bivalves, with special reference to artificial aquaculture systems", Aquaculture, 13, pp.1~33 (1978)
- Yoo, J.S., "Korean shells in color", pp.196~ (1976), Iljisa Pub. Co.