대복, Gomphina veneriformis의 생존 및 저질 잠입성에 미치는 TBTCl의 독성
Tributyltin chloride (TBTCl) Toxicity on the Survival Rate and Burrowing Activity of the Equilateral Venus, Gomphina veneriformis (Bivalvia: Veneridae)
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초록
This study was conducted to find out biological responses of bivalves exposed to organotin compound. The results of the study confirmed that tribultyltin chloride (TBTCl) induce reduction of survival rate and burrowing activity, and histopathological feature in the foot structure of the equilateral venus, Gomphina veneriformis. The experimental period was 36 weeks. The experimental groups consisted of a control and 3 TBTCl exposure groups (0.4 ㎛ TBTCl L-¹, 0.6 ㎛ TBTCl L-¹, 0.8 ㎛ TBTCl L-¹). The survival rate and activity were recorded daily. For histological analysis, foot tissues were fixed in Bouin’s fluid and then stained H-E stain and AB-PAS (pH 2.5) reaction after having serially sectioned the tissue by paraffin method at thickness of 4~6 ㎛. The survival rate was not significantly different among the control and exposure groups for 20 weeks, but in 0.8 ㎛ TBTCl L-¹, it was on the decreased ever since the exposure. The burrowing activity was not significantly different in the exposure group compared to the control up to 12 weeks, but in 0.6, 0.8 ㎛ TBTCl L-¹, it measured the lowest level after 20 weeks. The foot is composed of the epidermal layer, connective tissue, and muscular layer. The epidermal layer is composed of simple columnar, cuboidal epithelium and mucous cells. The cilia were well developed on the apical surface of epithelium. Circular, longitudinal and transverse muscle bundle were well developed in the muscular layer. The majority mucous cells showed blue color (542c) when it subjected to AB-PAS (pH 2.5) reaction. No histopathological alterations in the foot were observed before 12 weeks of exposure. After 20 weeks of exposure to 0.8 ㎛ TBTCl L-¹, the foot samples of exposed G. veneriformis showed disappearance of cilia and striated border partially and extension of hemolymph sinus. The mucous cell increased in the marginal of foot. At 28-week of exposure to 0.4 ㎛ TBTCl L-1, it observed weekly acid (564c), neutral (264c) and mixed mucous cell. At 36-week of exposure to 0.6 ㎛ TBTCl L-1, it showed fragmentation of the muscle and collagen fiber bundle, and also diappearance of cilia on epithelia and edema of epithelium in 0.8 ㎛ TBTCl L-¹.
Abstract
This study was conducted to find out biological responses of bivalves exposed to organotin compound. The results of the study confirmed that tribultyltin chloride (TBTCl) induce reduction of survival rate and burrowing activity, and histopathological feature in the foot structure of the equilateral venus, Gomphina veneriformis. The experimental period was 36 weeks. The experimental groups consisted of a control and 3 TBTCl exposure groups (0.4 ㎛ TBTCl L-¹, 0.6 ㎛ TBTCl L-¹, 0.8 ㎛ TBTCl L-¹). The survival rate and activity were recorded daily. For histological analysis, foot tissues were fixed in Bouin’s fluid and then stained H-E stain and AB-PAS (pH 2.5) reaction after having serially sectioned the tissue by paraffin method at thickness of 4~6 ㎛. The survival rate was not significantly different among the control and exposure groups for 20 weeks, but in 0.8 ㎛ TBTCl L-¹, it was on the decreased ever since the exposure. The burrowing activity was not significantly different in the exposure group compared to the control up to 12 weeks, but in 0.6, 0.8 ㎛ TBTCl L-¹, it measured the lowest level after 20 weeks. The foot is composed of the epidermal layer, connective tissue, and muscular layer. The epidermal layer is composed of simple columnar, cuboidal epithelium and mucous cells. The cilia were well developed on the apical surface of epithelium. Circular, longitudinal and transverse muscle bundle were well developed in the muscular layer. The majority mucous cells showed blue color (542c) when it subjected to AB-PAS (pH 2.5) reaction. No histopathological alterations in the foot were observed before 12 weeks of exposure. After 20 weeks of exposure to 0.8 ㎛ TBTCl L-¹, the foot samples of exposed G. veneriformis showed disappearance of cilia and striated border partially and extension of hemolymph sinus. The mucous cell increased in the marginal of foot. At 28-week of exposure to 0.4 ㎛ TBTCl L-1, it observed weekly acid (564c), neutral (264c) and mixed mucous cell. At 36-week of exposure to 0.6 ㎛ TBTCl L-1, it showed fragmentation of the muscle and collagen fiber bundle, and also diappearance of cilia on epithelia and edema of epithelium in 0.8 ㎛ TBTCl L-¹.
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