기내 콜히친 처리에 의한 염색체 배가 감 식물체 유기
Induction of A Chromosome-doubled Persimmon (Diospyros kaki Thunb.) by in vitro Colchicine Treatment
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본 시험은 최근 소비트렌드의 변화에 따라 소비자들이 쉽고편하게 먹을 수 있는 씨 없는 감 개발의 선결조건인 배수체(12x) 교배 모본을 선발하기 위해 수행되었다. 염색체가 배가된 배수체 개체를 얻기 위해 교배 종자의 배배양을 통해 얻은 유묘의 생장점에 콜히친을 처리하였다. 콜히친을 0.03, 0.05%, 0.1% 등3수준으로 처리한 결과, 0.05%에서 염색체가 배가된 개체의 발생률이 가장 높았다. 콜히친 처리 후 우리는 신초와 뿌리의 발생을 유도하기 위한 배지 조건을 구명하는 시험을 수행하였다. 신초는 10 μM, 30 zeatin이 각각 첨가된 1/2MS배지에서 생장이잘 되었으며, 뿌리는 10 mM NAA+5% DMSO에 5초 동안 침지후 1/2MS배지에 배양하였을 때 생장이 잘 되었다. 또한 콜히친처리에 의해 염색체가 배가된 개체(12x)의 기공 특성을 조사한결과 염색체가 배가된 개체(12x)는 염색체 배가가 되지 않은 개체(6x)와 교배친(6x) 보다 잎의 공변세포 밀도는 더 적고, 크기는 더 큰 경향이었다.
Abstract
This was carried out to develop a chromosome-doubled (12x) persimmon that will be used as a crossing parent to select seedless persimmon cultivars with the change of the consumption trend recently. To obtain a chromosome-doubled (12x) persimmon, colchicine was applied at the meristem of seedlings in vitro derived from cross among hexaploid persimmon (Diopyros kaki Thunb.). These were treated with 0.03%, 0.05% and 0.1% colchicine respectively for doubling chromosome, and it was most effective at the concentration of 0.05% colchicine. After colchicine treatment, we conducted tests to elucidate conditions for inducing shoot and root development. As the result, the shoots grew best when cultivated at 1/2MS media plus 10 and 30 μM zeatin respectively, and the roots grew best when cultivated at 1/2MS media after dipping for 5 seconds at 10 mM NAA+5% DMSO. We also compared seedlings that have chromosome (6x) do not doubled and crossing parents (6x) and chromosome-doubled seedlings (12x). As the result, these chromosome-doubled seedlings (12x) showed lower stomatal density and larger stomatal size.
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