본문 바로가기
KERD
논문

배수체 작성에 따른 시호 작물 특성

Colchicine-Induced Polyploidy and It’s Agronomic Characters in Bupleurum falcatum

이상철|정일경|손태권
한국약용작물학회|2008.02|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제16권 제1호|pp. 39-43

1

조회

0

다운로드

1

인용

0

참고문헌

키워드

Agronomic charactersBupleurum falcatumcolchicineFlow CytometryPolyploid

초록

The effect of colchicine treatment on the agronomic performance and polyploid formation of Bupleurum falcatum using flow cytometry technique was investigated. The roots of 4-leaf stage plants were treated with colchicine (0.5%) for 3, 6, 12, and 24 hours and then transplanted in the field. Agronomic characters (survival rate, plant height, chlorophyll content, bolting rate) were recorded at 4 weeks and 6 months after transplanting while flow cytometry technique was conducted for determination of polyploid formation. Flow cytometry technique revealed polyploid nuclear DNA formation in colchicine treated plants. The highest number of polyploids was obtained at the shortest colchicine treatment time indicating an inverse relationship between colchicine treatment time and polyploid formation. Results also showed that survival and bolting rates were inversely related with the treatment time while plant height and chlorophyll were not significantly affected by the treatment. This study showed a convenient method for determination of colchicine-induced polyploid in B. falcatum and its superior agronomic performance at shorter treatment time.

Abstract

The effect of colchicine treatment on the agronomic performance and polyploid formation of Bupleurum falcatum using flow cytometry technique was investigated. The roots of 4-leaf stage plants were treated with colchicine (0.5%) for 3, 6, 12, and 24 hours and then transplanted in the field. Agronomic characters (survival rate, plant height, chlorophyll content, bolting rate) were recorded at 4 weeks and 6 months after transplanting while flow cytometry technique was conducted for determination of polyploid formation. Flow cytometry technique revealed polyploid nuclear DNA formation in colchicine treated plants. The highest number of polyploids was obtained at the shortest colchicine treatment time indicating an inverse relationship between colchicine treatment time and polyploid formation. Results also showed that survival and bolting rates were inversely related with the treatment time while plant height and chlorophyll were not significantly affected by the treatment. This study showed a convenient method for determination of colchicine-induced polyploid in B. falcatum and its superior agronomic performance at shorter treatment time.

참고문헌 (28)

  1. Arumuganathan K, "Estimation of nuclear DNA content of plants by flow cytometry", Plant Mol. Biol. Rep, 9, pp.221~231 (1991), 221-231.
  2. Bino RJ, "Flow cytometric determination of nuclear replication stages in seed tissues", Ann. Bot, 72, pp.181~187 (1993)
  3. Blakeslee AF, "Methods of inducing doubling of chromosomes in plants", J. Hered, 28, pp.393~411 (1937)
  4. 허권, "한국특산식물 섬시호의 해부학적 연구", 한국약용작물학회지, 14(6), pp.342~346 (2006), 한국약용작물학회
  5. Dolezel J, "Plant DNA flow cytometry and estimation of nuclear genome size", Ann. Bot, 95, pp.99~110 (2005)
  6. De Jesus-Gonzalez L, "Tetraploid Artemisia annua hairy roots produce more artemisinin than diploids", Plant Cell Rep, 21, pp.809~813 (2003)
  7. Eigsti OJ, "cytological study of colchicine effects in the induction of polyploidy in plants", Proc. Natl. Acad. Sci. USA, 25, pp.56~63 (1938)
  8. Gao SL, "In vitro production and identification of autotetraploids of Scutellaria baicalensis", Plant Cell Tiss. Org, 70, pp.289~293 (2002)
  9. Hassan L, "Colchicine-induced variation of cell size and chloroplast number in leaf mesophyll of rice", Plant Breeding, 119, pp.531~533 (2000)
  10. Hosoda K, "Shoyakugaku Zasshi", 47(1), pp.17~21 (1992)
  11. Kim IH, "Breeding of tetraploid in Platycodon grandiflorum (Jacq.) A. DC. by colchicine treatment", Korea J. Plant Res, 6(3), pp.188~194 (2003)
  12. Choon Shik Kim, "Root Yields and Saikosaponin Contents Depending onPlanting Time and Cultivated Regions of Bupleurumfalcatum L.", 한국약용작물학회지, 13(5), pp.298~302 (2005), 한국약용작물학회
  13. 이중구, "체세포분열과 감수분열 및 bicolor FISH를 이용한 섬시호의세포유전학적 분석", 한국약용작물학회지, 14(6), pp.354~359 (2006), 한국약용작물학회
  14. Lapins KO, "Polyploidy and mutations induced in apricot by colchicine treatment", Can. J. Genet. Cytol, 17, pp.591~599 (1975)
  15. Madon M, "Polyploid induction of oil palm through colchicine and oryzalin treatments", J. Oil Palm Res, 17, pp.110~123 (2005)
  16. Minami M, "Seasonal variation on growth and saikosaponins concentration of Bupleurum falcatum L", Yakugaku Zasshi, 115(2), pp.145~155 (1995)
  17. Navarro-Alvarez W, "Addition of colchicine to wheat anther culture media to increase doubled haploid plant production", Plant Breeding, 112(3), pp.192~198 (1994)
  18. Obute CG, "Cytogenetic studies on some Nigerian species of Solanum L. (Solanaceae)", Afr. J. Biotech, 5(9), pp.689~692 (2005)
  19. Otsuka H, "Studies on the cultivation of Bupleurum falcatum L. (Mshimasaiko)", Shoyakugaku Zasshi, 31(2), pp.195~197 (1977)
  20. Ramachandran K, "Polyploidy induced in ginger by colchicine treatment", Curr. Sci. India, 51(6), pp.288~288 (1982)
  21. Rauf S, "Colchicine-induced tetraploidy and changes in allele frequencies in colchicine treated populations of diploids assessed with RAPD markers in Gossypium arboreum L", Turk. J. Biol, 30, pp.93~100 (2006)
  22. Shimokawa Y, "Cultivation and breeding of Bupleurum falcatum L. (I) Effect of temperature on growth, development and saikosaponin content of one-yearold plants", Shoyakugaku Zasshi, 34(3), pp.209~214 (1980)
  23. Shon TK, "Variation and distribution of saikosaponin in Bupleurum falcatum L", J. Fac. Agric. Kyushu Univ, 42, pp.17~22 (1997)
  24. Shon TK, "Induction of haploid plantlets by anther culture of Bupleurum falcatum L", Jpn. J. Crop Sci, 66, pp.137~138 (1997)
  25. Stebbins GL, "Polyploidy and the distribution of arctic-alpine flora: new evidence and new approaches", Bot. Helvestica, 94, pp.1~13 (1984)
  26. Tadato T, "Histochemistry XI. Histological and chemical characteristics of bolting and non-bolting roots of cultivated Bupleurum falcatum L", Chem. Pharm. Bull, 35(11), pp.4530~4536 (1987)
  27. Valkonen JP, "Analysis of correlation between nuclear DNA content, chromosome number, and flowering capacity of asymmetric somatic hybrids of diploid Solanum brevidens and (di)haploid S. tuberosu", Jpn. J. Genet, 69, pp.525~536 (1994)
  28. Yan G, "Chromosome doubling of wax flower. Plant regenerated in vitro", The breeding biology of wax flower, 11~20 (2001)

관련 논문AI추천

Colchicine 처리에 의해 유기된 4배체 하수오의 형태 및 세포유전학적 특성김기현, 김영호, 김인재, 우선희, 윤철구, 이경자, 홍성택한국약용작물학회 · 한국약용작물학회지2018 · 제26권 제5호Effect of Colchicine on Chromosome Doubling in Codonopsis lanceolata권수정, 김학현, 문영자, 부희옥, 서동연, 우선희, 이문순, 조갑연한국자원식물학회 · 한국자원식물학회지2016 · 제29권 제3호콜히친 침지처리에 의한 ‘망종화’의 4배체 식물유도권수정, 김학현, 조갑연한국자원식물학회 · 한국자원식물학회지2013 · 제26권 제2호염색체 배수화제를 이용한 4배체 수박품종 개발김영철, 민광현, 박상빈, 오상아, 조송미, 최용수한국자원식물학회 · 한국자원식물학회지2015 · 제28권 제5호식물생장조절물질 처리에 따른 시호의 생육특성이호, 김길웅, 손태권, 이상철, 이지언한국약용작물학회 · 한국약용작물학회지2002 · 제10권 제5호기내 콜히친 처리에 의한 염색체 배가 감 식물체 유기강삼석, 마경복, 서호진, 정해원, 조광식한국자원식물학회 · 한국자원식물학회지2018 · 제31권 제5호

이 간행물의 다른 논문 (9건)

제주 손바닥 선인장의 초음파 추출을 통한 면역활성 증진정향숙, 이현용, 고정림, 최영범, 권민철, 한재권, 임태일, Syed Abdul Qadirpp. 1-8초음파 추출물을 이용한 치콘의 면역활성 증진Syed Abdul Qadir, 이현용, 안주희, 이달호, 권민철, 한재권pp. 9-15정식시기에 따른 바위솔의 생장과 개화이창우, 김홍영, 신성철, 강진호, 전승호pp. 16-19엽록체 DNA 염기서열을 이용한 한약재 지모의 기원 확인 및 유연관계 분석김명겸, 김세영, 양덕춘, 노종훈, 손화, 베갈마pp. 20-26Syk kinase 억제를 통한 관중의 항앨러지 효과김영미pp. 27-32
원문 보기