Priming 처리조건이 광나무 종자의 발아 및 유묘 생육에 미치는 영향
Effect of Priming Treatment Conditions Germination and Early Growth of Ligustrum Japonicum Thunb. Seeds
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
본 연구는 광나무(Ligustrum japonicum) 종자의 파종 효율성 및 유묘 생산성을 증진시키기 위하여, 발아와 균일성에 효과가 있는 priming 처리 중 처리시간, 처리제 및 처리농도를 비교·분석하여 적절한 전처리 조건을 구명하고자 실시하였다. 광나무 종자는 충실 종자를 사용하기 위해1일간 침지처리 후 침강 종자만을 사용하였다. 종자 전처리는 priming 처리시간(24, 48시간)에 따라 GA3 (0, 10, 100, 200 ㎎·L-1) 생장조절제와Ca(NO3)2 및 KNO3 (0, 50, 100, 200 mM) 무기염류로 처리하여 시험을 수행하였다. 발아특성을 분석한 결과 priming 24시간 처리는 대조구보다유의적으로 높은 발아율, 발아속도, 발아균일지수를 보인 반면(p≤0.001), priming 48시간 처리 시 대조구보다 낮은 발아특성을 나타냈다. 특히, priming 24시간·GA3 200 ㎎ 처리에서 발아율 44.0%, 발아속도 0.59%·day, 발아균일지수 0.55으로 가장 높은 효과를 보였다(p≤0.001). 한편, priming 48시간·GA3 및 Ca(NO3)2 200 mM 처리구에서는 발아가 나타나지 않았다. priming 처리된 종자로부터 생장한 유묘들을 대상으로 T/R율및 유묘활력지수를 조사한 결과 발아율이 가장 높았던 priming 24시간·GA3 200 ㎎ 처리에서 낮은 T/R율과 수분감소율을 보였으며, 유묘 활력지수는793.0으로 가장 높게 나타났다(p≤0.001). 결과적으로 priming 종류(GA3, Ca(NO3)2, KNO3) 중 광나무 종자 발아 및 유묘의 생장에 영향을적정 처리제는 GA3로 판단되며, 적정 처리 조건은 200 ㎎, 24시간 처리로 나타났다.
Abstract
The purpose of this study is to determine the proper pretreatment condition by comparing and analyzing the treatment time, type and density during priming treatment which is effective to germination and uniformity to improve the seedling effectiveness and productivity of Ligustrum japonicum. To obtain good quality seedling, after immersion treatment for 1 day, only sedimented seeds were used. For pretreatment, the test was conducted by treating GA3 (0, 10, 100, 200 ㎎·L-1) growth regulator and Ca(NO3)2 and KNO3 (0, 50, 100, 200 mM)inorganic salts according to priming treatment hour(24, 48 hours). As a result of analyzing germination feature, 24 hour priming treatment showed Germination rate, Germination speed, Germination performance index (p≤0.001) which are significantly higher than control group while 48 hour priming treatment showed the germination feature that is lower than control group. In particular, 24 hour priming and GA3 200 ㎎ treatment showed the highest effect (p≤0.001) such as Germination rate 44.0%, Germination speed 0.59%·day, Germination performance index 0.55. On the contrary, 48 hour priming, GA3 and Ca(NO3)2 200 mM treated specimen showed no germination. As a result of researching T/R ratio and Seedling Vitality Index targeting seedling from priming treated seeds, 24 hour priming GA3 200 ㎎ treatment which showed the highest germination rate, showed low T/R rate and reduction rate, and Seedling Vigor Index is 793.0 which is the highest (p≤0.001). In conclusion, the proper treatment agent that influences to the germination and growth of Ligustrum japonicum among priming type (GA3, Ca(NO3)2, KNO3) is judged to GA3 and proper treatment condition is found to be 200 ㎎, 24 hours.
참고문헌 (26)
- Abdul-Baki AA, "Vigor determination in soybean seed by multiple criteria 1", Crop Science, 13(6), pp.630~633 (1973)
- Brocklehurst PA, "A comparison of different chemicals changes during osmotic treatment of vegetable seed", Annals of Applied Biology, 105(2), pp.391~398 (1984)
- Carpenter WJ, "Priming improves high temperature germination on pansy seed", Hortsci, 26(5), pp.541~544 (1991)
- 최충호, "PEG를 이용한 priming 처리가 고로쇠나무 종자 발아에 미치는 영향", 한국자원식물학회지, 19(4), pp.480~4 (2006), 한국자원식물학회
- Coolbear P, "The effect of low temperature pre-sowing treatment on the germination performance and membrane integrity of artificially aged tomato seeds", J. of Experimental Botany, 35(11), pp.1609~1617 (1984)
- Dahal P, "Effects of priming and endosperm integrity on seed germination rates of tomato genotypes: I. Germination at suboptimal temperature", J. of Experimental Botany, 41(11), pp.1431~1439 (1990)
- Stimulation of black spruce germination by osmotic priming: Laboratory studies. 31, "Fleming LS", Great Lakes Forest Reserch Center, Canadian Forestry Service, ~, pp.Information Report O-X-362 (1984)
- 한심희, "지베렐린 처리에 따른 서어나무 16년 저장 종자의 휴면타파 및 발아촉진 효과", 한국산림과학회지, 108(4), pp.531~539 (2019), 한국산림과학회[DOI]
- Heydecker W, "Seed treatment for improved performance-survey and attempted prognosis", Seed Science and Technology, 5, pp.353~425 (1977)
- Heydecker W, "Invigoration of seeds", Seed Science and Technology, 3, pp.881~888 (1975)
- "Bio-industry and regulation" (2020)[URL]
- 정인창, "광나무 잎의 페놀성 화합물", 한국식품영양과학회지, 35(6), pp.713~720 (2006), 한국식품영양과학회
- An analysis on the source of innovation for the new Korean Wave: Focusing on K-beauty, "Jung IY", Science and Technology Policy Institute, ~, pp.222 (2020)
- Kang JS, "Effect of priming on the germinablility of watermelon(Citrullus vulgaris Schrad)seeds and seedling growth", Horticulture, Environment, and Biotechnology, 37(1), pp.12~18 (1996)
- 임효인, "저온습윤 및 지베렐린 처리가 눈잣나무의 종자발아에 미치는 영향", 한국자원식물학회지, 28(2), pp.253~258 (2015), 한국자원식물학회[DOI]
- 임순성, "상록활엽수 정유성분의 GC", MS 분석, 2008, pp.21(4), 237~248[DOI]
- Manangkil OE, "A simple, rapid and reliable bioassay for evaluating seedling vigor under submergence in indica and japonica rice (Oryza sativa L.)", Euphytica, 163(2), pp.267~274 (2008)
- Mishra A, "Seedling characteristics and the early growth of transplanted rice under different water regimes", Experimental Agriculture, 44(3), pp.365~383 (2008)
- Current status and challenges of the biomaterial industry in the agricultural sector, "Park JH", Korea Rural Economic Institute, ~, pp.181, Research Report R815 (2017)
- Research trends and prospects of forest medicinal resources, "Park YM", National Institute of Forest Science, Korea Forest Service, ~, pp.20, NIFoS Forest Policy Issue No. 89 (2017)
- Scott SJ, "Review of data analysis methods for seed germination", Crop Science, 24(6), pp.1160~1162 (1984)
- 서병수, "Priming 처리가 팥배나무의 종자 발아 및 유묘 생장에 미치는 영향", 한국자원식물학회지, 22(1), pp.5~12 (2009), 한국자원식물학회
- Stundstrom FJ, "Effect of seed treatment and planting method on tabasco pepper", J. of the American Society for Horticultural Science, 112(4), pp.641~644 (1987)
- Suzuki H, "Effect of pH of tertiary phosphate solutions on radicle protrusion during priming of carrot seeds", J. of the Japanese Society for Horticultural Science, 59(3), pp.589~595 (1990)
- Taylor AG, "Seed enhancements", Seed Science Research, 8(2), pp.245~256 (1998)
- Yoon JH, "The study on growth characteristics and cultivated methods for Acer pictum complex in Korea", Ph.D., pp.2013