방사선에 의한 암세포주 특이적 유전자 발현 양상
Cell-type-specific Gene Expression Patterns in Human Carcinoma Cells followed by Irradiation
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Abstract
- Ionizing radiation is a well-known therapy factor for human carcinoma cells.Genotoxic stress mediates cell cycle control, transcription and cellular signaling. In this work, wehave used a microarray hybridization approach to characterize the cell type-specifictranscriptional response of human carcinoma MCF- γ-radiation, such as4 Gy 4hr. We found that exposure to γ-ray alters by at least a log2 factor of 1.0 the expression ofknown genes. Of the 27 genes affected by irradiation, 11 are down-regulated in MCF-7cells anddamage-response pathways for cell cycling, transcription factor and cellular signaling response.However, in MCF-7 cells, we observed gene expression pattern in chromatin, apoptosis, stress,differentiation, cytokine, metabolism, ribosome and calcium. In HeLa cells, it showed clearly theexpression changes in adhesion and migration, lysosome, brain, genome instability andtranslation. These insights reveal new therapy directions for studying the human carcinoma cellresponse to radiation.
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