MCF-7 세포주의 ？선에 의한 DNA 손상 반응 유전자 발현 양상의 분석
A DNA-Damage Response Gene Expression Analysis in MCF-7 followed by r-Radiation
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Abstract
- Cell response to genotoxic agents is complex and involves the participation of differentpresented a approach to characterize the cellular functions associated with the altered transcriptprofiles of MCF-7 exposed to low-dose in vitrogamma-irradiation. We used the method ofhuman 2.4 k cDNA microarrays containing apoptosis, cell cycle, chromatin, repair, stress andchromosome genes to analyze the differential gene expression characterization that weredisplayed by radiation- -7 cell line, such as 4 Gy 4hr, 8 Gy 4 hr, and 8 Gy 12 hr. Among these genes, 66 were up-regulated and 49 were down-regulated. Specific genes were concomitantly induced in the results. Cyclin dependent kinase 4(Cdk4) is induced for starting the cell cycle. This regulation is required for a DNA damage-induced G1 arrest. In addition to, an apoptotic pathways gene Bcl-w was concomitantly induced.Mismatch repair protein homologue-1 (hMLH1), a necessary component of DNA mismatchprotein repair (MMR), in G2-M cell cycle checkpoint arrest. The present study provides newinformation on the molecular mechanism underlying the cell response to genotoxic stress, withrelevance to basic and clinical research.
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