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간암세포주에서 지속적인 p16 단백질발현이 유도하는 유전자발현의 변화

Gene Expression Changes Associated with Sustained p16 Expression in Hepatocellular Carcinoma Cells

임지영|이용복|정제훈|오상진
대한면역학회 (The Korean Association of Immunologists)|2004.12|Immune Network|제4권 제4호|pp. 237-243

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Abstract

Background: The normal functions of the cell cycle inhibitor p16INK4a are frequently inactivated in many human cancers. Over 80% of hepatocellular carcinoma (HCC) cases lack a functional p16/Rb pathway. p16/Rb pathway, as well as p53 pathway, is considered as one of key components of tumor suppression. Methods: To study the roles of p16INK4a in HCC, a stable cell line expressing exogenous p16 was generated from SNU-449 hepatocellular carcinoma cells lacking endogenous p16, and suppression subtractive hybridization (SSH) was performed in parallel with the control cells. Results: 1) SSH identifies fibronectin (FN1), crystallin αB (CRYAB), Rac1, WASP, RhoGEF, and CCT3 as differentially-expressed genes. 2) Among the selected genes, the up- regulation of FN1 and CRYAB was confirmed by Northern blot, RT-PCR and by proteomic methods. Conclusion: These genes are likely to be associated with the induction of stress fiber and stabilization of cytoskeleton. Further studies are required to clarify the possible role of p16 in the signal transduction pathway. (Immune Network 2004;4(4):237-243)

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관련 논문AI추천

Cyclin-dependent Kinase 저해 단백질 p16INK4A의 인체 암세포에서의 세포사멸 유도 활성김민경, 이철훈한국미생물·생명공학회 · 한국미생물·생명공학회지2004 · 제32권 제1호방사선에 의한 암세포주 특이적 유전자 발현 양상김진규, 박지윤, 채영규한국환경생물학회 · 환경생물2005 · 제23권 제2호간암 세포주 및 위암 세포주에서 Nonsteroidal Anti-inflammatory Drug Activated Gene (NAG-1)의 기능 연구장태정대한병리학회 · Journal of Pathology and Translational Medicine2007 · 제41권 제4호대장암 및 대장선종에서 p16 유전자의 과메틸화조몽, 김대현, 김도하, 박능화, 양웅석, 주광로대한내과학회 · 대한내과학회지2003 · 제64권 제4호Real-time quantitative PCR법을 이용하여 두경부 편평암 세포주에서 과발현되는 유전자규명강구성, 김정옥, 김정완, 손윤경, 윤상모, 이용규, 이해암, 장현중, 전소영대한구강악안면외과학회 · 대한구강악안면외과학회지2006 · 제32권 제1호간세포암종에서 TPEF의 Methylation에 의한 불활성화강구성, 김순영, 김정옥, 김정완, 박지영, 손윤경, 이해암, 전소영, 정운복대한병리학회 · Journal of Pathology and Translational Medicine2008 · 제42권 제1호

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