호박과실의 성숙과정에 따른 항산화효소의 활성변화
Changes in the activity of Antioxidant Enzymes Depending on Ripening Levels in Cucurbita spp.
0
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
본 연구는 서양계 단호박과 동양계 늙은 호박의 성숙과정에 따른 탄수화물의 축적 양상과 항산화효소의 활성 변화를 조사하기 위하여 실시한 결과, 종간 차이가 뚜렷하게 나타났다. 단호박 과실의 건물률과 탄수화물은 수확기까지 지속적으로 높게 유지된 반면, 늙은 호박은 60일째까지 큰 변화 없이 단호박보다 낮았다. 이는 호박 종간에 과실의 성숙과정이 차이가 있음을 나타내었다. 두 종의 과실의 유리당을 비교하였을 때, 늙은 호박 과실에서는 과당과 포도당이, 단호박 과실에서는 자당이 높았다. 착과 후 발육시기에 따른 호박 과실의 종간 항산화효소(SOD, HPX, APX) 발현 양상은 뚜렷한 차이를 보였다. HPX의 경우 종간에 밴딩 패턴 차이와 더불어 시기에 따른 밴드 밀도에 차이가 있었으나, SOD와 APX 겔 활성은 호박 종간 패턴상의 차이는 없었지만 시기에 따른 발현 밀도 차이는 있었다. 따라서 본 연구결과는 호박 과실의 품질향상과 수확시기 판정 등에 이용될 수 있는 기초자료로 유용하게 활용될 것으로 판단된다.
Abstract
This study was conducted to investigate accumulation patterns of carbohydrate and changes in the activity of antioxidant enzymes depending on levels of ripening in Cucurbita maxima and Cucurbita moschata. The results of this research showed that differences between species were evident. Dry matter and total carbohydrates in Cucurbita maxima maintained continuously in a high levels until harvest stage. While in Cucurbita moschata stayed in a low levels until 60 days. The results indicated differences between species in fruit maturation. Fructose and glucose contents in fruits were higher in Cucurbita moschata, however the accumulation of sucrose were higher in Cucurbita maxima. There was considerable variability in antioxidant enzyme (SOD, HPX, APX) expression patterns in fruit of Cucurbita species. HPX banding pattern and banding density according to ripening levels in fruits of tested species were different. SOD and APX gel activity of banding pattern between Cucurbita species did not differ, but banding density in ripening times was different. SOD and APX gel activity of banding pattern between Cucurbita species were not different but banding density on maturity levels was different. Our results can be useful as a basic data on improvement of fruit quality and determination of harvest time of Cucurbita species.
참고문헌 (31)
- Anderson, M. D., "Changes in isozyme profiles of catalase, peroxidase, and glutathione reductase during acclimation to chilling in mesocotyls of maize seedlings", Plant Physiol, 109, pp.1247~1257 (1995)
- Arrigoni, O, "Ascorbate system in plant development", Journal of bioenergetics and biomembranes, 26, pp.407~419 (1994)
- Asada, K, "Ascorbate peroxidase: A hydrogene peroxide-scavenging enzyme in plants", Physiol. Plant, 85, pp.235~241 (1992)
- Awad, M. A., "Antioxidant capacity, antioxidant compounds and antioxidant enzyme activities in five date cultivars during development and ripening", Sci. Hort, 129, pp.688~693 (2011)
- Baker, J. E, "Superoxide dismutase in ripening fruits", Plant Physiol, 58, pp.644~647 (1976)
- Beauchamp, C., "A mechanism for the production of ethylene from methional. The generation of the hydroxyl radical by xanthine oxidase", J. Bio. Chem, 245, pp.4641~4646 (1970)
- Bradford, M.M., "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", Anal. Biochem, 72, pp.248~254 (1976)
- Brennan, T., "Involvement of hydrogen peroxide in the regulation of senescence in pear", Plant Physiol, 59, pp.411~416 (1977)
- Chen, G.X., "Ascorbate peroxidase in tea leaves : Occurrence of two isozymes and the differences in their enzymatic and molecular properties", Plant Cell Physiol, 30, pp.987~998 (1989)
- Davies, K.J.A., "Oxidative stress: The paradox of aerobic life", Biochem. Soc. Symp, 61, pp.1~32 (1995), Portlant Press
- Foyer, C.H., "Antioxidants in higher plants", pp.31~58 (1993), CRC Press
- oxidative stress and plant metabolism, "Active oxygen", pp.1991, Fridovich, I.
- Hu, C., "Role of carbohydrate moieties in peanut peroxidases", Biochem. J, 263, pp.129~135 (1989)
- Hubbard, N.L., "Sucrose phosphate synthase and acid invertase as determinants of sucrose concentration in developing muskmelon (Cucumis melo L.) fruits", Plant Physiol, 91, pp.1527~1534 (1989)
- Huang, R., "Antioxidant activity and oxygen-scavenging system in orange pulp during fruit ripening and maturation", Sci. Hort, 113, pp.166~172 (2007)
- Jimenez, A., "Changes in oxidative processes and components of the antioxidant system during tomato fruit ripening", Planta, 214, pp.751~758 (2002)
- 강남준, "단호박과 국수호박의 과실발달에 따른 탄수화물 축적과 효소 활성 변화", Horticulture, Environment, and Biotechnology, 43(5), pp.571~574 (2002), 한국원예학회
- Lee, D.H., "Chilling stress-induced changes of antioxidant enzymes in the leaves of cucumber : in gel enzyme activity assays", plant science, 159, pp.75~85 (2000)
- Mittler, R., "Detection of ascorbate peroxidase activity in native gels by inhibition of the ascorbate dependent reduction of nitroblue tetrazolium", Anal. Biochem, 212, pp.540~546 (1993)
- Nelson, N., "A photometric adaptation of the Somogyi method for the determination of glucose", J. Biol. Chem, 153, pp.375~380 (1944)
- Nielsen, T.H., "Carbohydrate metabolism during fruit development in sweetpepper (Capsicum annuum) plants", Physiol. Plant, 82, pp.311~319 (1991)
- Olson, P.D., "Hydrogen peroxide and lignification", Plant J, 4, pp.887~892 (1993)
- Paciolla, C., "Distribution of cytosolic ascorbate peroxidase in angiosperms", Plant Biosystem, 130, pp.729~737 (1996)
- Palafox-Carlos, "Effect of ripeness stage of mango fruit (Mangifera indica L., cv. Ataulfo) on physiological parameters and antioxidant activity", Sci. Hort, 135, pp.7~13 (2012)
- Rao, M.V., "Ultraviolet-B-and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana", Plant Physiol, 110, pp.125~136 (1996)
- Razzaq, K., "Ripening period influences fruit softening and antioxidative system of ‘Samar Bahisht Chaunsa’ mango", Sci. Hort, 160, pp.108~114 (2013)
- Salin, M. L, "Chloroplast and mitochondrial mechanism for protection against oxygen toxicity", Free Radic. Res. Commun, 13, pp.851~858 (1991)
- Tan, C. K., "Changes in ethylene production, carbohydrase activity and antioxidant status in pepper fruits during ripening", Scientia Horticulturae, 142, pp.23~31 (2012)
- Walker, A.J., "Carbon translocation in tomato: carbon import and fruit growth", Ann. Bot, 41, pp.813~823 (1977)
- Wang, F. A., "Sucrose synthase, starch accumulation, and tomato fruit sink strength", Plant Physiol, 101, pp.321~327 (1993)
- Zushi, K., "Developmental and tissue-specific changes in oxidative parameters and antioxidant systems in tomato fruits grown under salt stress", Sci. Hort, 122, pp.362~368 (2009)