본문 바로가기
KERD
논문KCI 등재

미치광이풀 세포의 기관분화에 따른 트로판알칼로이드 함량과 세포미세구조의 변화

Changes of ultrastructure of cells and tropane alkaloid contents during organogenesis of Scopolia parviflora callus cultures

임영길|최명석|김용덕
농업생명과학연구원 (Institute of Agriculture & Life Science, Gyeongsang National University)|2014|농업생명과학연구 (Journal of Agriculture & Life Science)|제48권 제1호|pp. 23-32|DOI http://dx.doi.org/10.14397/jals.2014.48.1.23

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

TEM분화Differentiation탈분화부정근 배양투과전자현미경 (TEM)DedifferentiationAdventitious root cultures

초록

미치광이풀 부정근으로부터 탈분화 및 재분화에 미치는 세포소기관의 형태 및 tropane alkaloids의 함량 변화를 TEM과 HPLC를 사용하여 조사하였다. Scopolamine and hyoscyamine 및 세포 소기관의 형태변화는 세포분화 및 탈분화 단계마다 차이를 보였다. 부정근은 배양 20일후에는 캘러스가 형성되었고, 배양 60일부터 줄기원기가 발생하였으며, 이후 완전한 줄기로 발달하였다. 부정근 정단부 조직을 TEM 분석 결과 핵, 액포, 전분체, 미토콘드리아, 골지체, 세포벽 등 다양한 세포소기관들이 형성되었으나, 배양 20일후부터는 소기관들이 적게 관찰되었다. 그러나 분화가 시작되는 배양 40일 후 callus 세포에는 다수의 전분체 등 다양한 세포소기관들이 다시 관찰되었고, 배양 60일 이후에는 다시 전분립의 수가 감소하였다. 배양 80일째 세포는 전분립이 거의 관찰되지 않았으나, 미토콘드리아, 골지체 등 다양한 세포소기관들이 다시 관찰되었다. 한편, scopolamine과 hyoscyamine 함량은 배양 60일부터 다소 증가하다가 이후 90일까지 급격히 증가하였고, 이후에는 급격한 감소를 보였다. 본 연구 결과 tropane alkaloid 생합성은 탈분화 및 분화 과정에 영향을 받으며, 세포 내 전분립 생성은 기관분화와 더불어 물질생합성과 매우 밀접한 관련성이 있을 것으로 추정된다.

Abstract

Ultrastructure of cell organell and tropane alkaloid production depend on dedifferentiation and differentiation of adventitious root from Scopolia parviflora were determined through TEM and HPLC analysis. Content of scopolamine and hyoscyamine and cell untrastructure were found to be changeable based on dedifferentiation and differentiation stages. Adventitious root induced callus after 20 days and formed organogenic shoot primodia after 40 days and then was grown into entire plantlets. Cells of adventitious root tips observed various cell organells as nucleus, vacuole, amyloplast, mitochondria, golgi apparatus and cell wall, however, some organs in dediferentiated callus was not observed after 20 days of cultures. Starch grain and some organells were re-observed in organogenic calli after 40 days of cultures. After 60 days of culture, starch grains in organogenic callus were decreased. However, cell organells as mitochondria and golgi apparatus were observed in the differentiating calli after 80 days of cultures. Content of scopolamine and hyoscyamine were somewhat increased until 60 days of cultures, however, it was dramatically increased until 90 days of cultures. Starch grain is major factor on cell differentiation and tropane alkoaloid biosynthesis of Scopolia parviflora adventitious root cultures.

참고문헌 (26)

  1. Constabel, F., "Plant Protoplasts", pp.209~217 (1983), Int Rev Cytolsuppl Academic Press
  2. Endo, T., "Tropane alkaloid production in cultured cells of Duboisia leichhardtii", Plant Cell Rep, 3, pp.186~188 (1984)
  3. Fortes, A. M., "Organogenesis from internode-derived nodules of Humulus lupulus var. Nugget : histological studies and changes in the starch content", Am J Bot, 87, pp.971~979 (2000)
  4. Hagemann, K., "Monoclonal antibody based enzyme immunoassay for the quantitative determination of the tropane alkaloid. Scopolamine", Planta, 58, pp.68~72 (1992)
  5. Hashimoto, T., "Progress in Plant Cellular and Molecular Biology", pp.775~ (1990), Kluwer Academic Publishers
  6. Hashimoto, T., "Production of tropane alkaloids in engineered root culture", Phytochem, 32, pp.713~718 (1993)
  7. Iranbakhsh, A., "Distribution of atropine and scopolamine in defferent organs and stages of development in Datura stramonium L. (Solanaceae)structure and ultrastructure of biosynthesizing cells", Acta Biolosia Cracoviensia Series Botanica, 48, pp.13~18 (2006)
  8. Iwase, A., "Development of a novel system for producing ajmalicine and serpentine using direct culture of leaves in Catharanthus roseus intact plant", J Biosci Bioeng, 99, pp.208~215 (2005)
  9. Jung, G., "Use of genetic transformation by the Ri T-DNA of Agrobacterium rhizogenes to stimulate biomass and tropane alkaloid production in Atropa belladonna and Calystegia sepium grown in vitro", Plant Sci, 50, pp.145~151 (1987)
  10. Jung, H. Y., "Optimal culture conditions and XAD resin on tropane alkaloid production in Scopolia parviflorahairy root cultures", Korean J Biotechnol Bioeng, 17(6), pp.525~530 (2002)
  11. Kang, S. M., "Effects of methyl jasmonate and salicylic acid on the production of tropane alkaloids and the expression of PMT and H6H in adventitious root cultures of Scopolia parviflora", Plant Sci, 166, pp.745~751 (2004)
  12. Kang, Y. M., "Enhanced production of tropane alkaloids in transgenic Scopolia parviflora hairy root cultures over-expressing putrescine N-methyl transferase(PMT) and hyoscyamine-6Bhydroxylase( H6H)", In Vitro Cell & Dev, 47, pp.516~524 (2011)
  13. Kim, I. S., "Structural aspects of seven leaves of Portulaca growing in Hawaii", Can. J. Bot, 68, pp.1803~1811 (1990)
  14. Kim, I. S., "Chloroplast microtubules in young leaves of Sedum rotundifolium", J Plant Biol, 40, pp.115~119 (1997)
  15. Kim, Y. D., "Production of tropane alkaloids during De-differentiation of Scopolia parviflora calli", J Nat Prod, 73, pp.147~150 (2010)
  16. Mangat, B. S., "Changes in the starch content during organogenesis in in vitro cultured Begonia rex stem explants", Physiologia Plantarum, 79, pp.267~274 (1990)
  17. Matsumoto, T., "Production of phytoecdysteroids by hairy root cultures of Atropa reptans var. atropurpurea", Agri. Biol. Chem, 55, pp.2715~2722 (1991)
  18. Min, J. Y., "Production of tropane alkaloids by small-scale bubble column bioreactor cultures of Scopolia parviflora adventitious roots", Bioresource Technol, 98, pp.1748~1753 (2007)
  19. Mishra, B. N., "Growth of hairy-root cultures in various bioreactors for the production of secondary metabolites", Biotechnol Appl Biochem, 49, pp.1~10 (2008)
  20. Peerless, C. J., "Enzymes of N-methylputrescine biosynthesis in relation to hyoscyamine formation in transformed root cultures of Datura stramonium and Atropa belladonna", Planta, 182, pp.136~141 (1990)
  21. Sahai, O. P., "Environmental parameters influencing phenolics production by batch cultures of Nicotiana tabacum", Biotechnol Bioeng, 26, pp.111~120 (1984)
  22. Saito, K., "Transgenic medicinal plants: Agrobacterium mediated foreign gene transfer and production of secondary metabolites", J. of Natural Products, 55, pp.149~162 (1992)
  23. Stamp, J. A., "Somatic embryogenesis in cassava : the anatomy and morphology of the regeneration process", Annals of Botany, 57, pp.451~459 (1987)
  24. Yamada, Y., "Production of tropane alkaloids in cultured cells of Hyoscyamus niger", Plant Cell Rep, 1, pp.101~102 (1982)
  25. Yamada, Y., "Plant biotechnoilogy of tropane alkaloids", Plant Biotechnol, 14, pp.1~10 (1997)
  26. Wink, M., "Accumulation of quinolizidine alkaloids in plants and cell suspension cultures : Genera Lupinus, Cytisus, Baptisia, Genista, Laburnum and Sophora", Planta Med, 48, pp.253~257 (1983)

관련 논문AI추천

미치광이풀 모상근 배양에서 Tropane Alkaloids 생산성 증진을 위한 최적 생물학적 엘리시터 선발강승미, 강영민, 김용덕, 양재경, 정영관, 정희영, 최명석한국약용작물학회 · 한국약용작물학회지2003 · 제11권 제5호Production of Tropane Alkaloids by Optimal CultureConditions in Adventitious Root of Hyoscyamus niger L.Dong Jin Park, Ha Na Jung, Hoon Serg Kang, Ji Yun Min, Myung Suk Choi, Seung Mi Kang, Yong Duck Kim한국약용작물학회 · 한국약용작물학회지2005 · 제13권 제5호Production of Tropane Alkaloids by Two-stage Culture of Scopolia parviflora Nakai Adventitious Root김운정한국약용작물학회 · 한국약용작물학회지2004 · 제12권 제5호Production of glycoalkaloids from callus cultures of Solanum hainanense Hance김태금, 양문식, Doan Huu Nhat Binh, Nguyen Hoang Loc, Nguyen Huu Thuan Anh한국식물생명공학회 · Journal of Plant Biotechnology2010 · 제37권 제1호이차대사물질의 생산을 위한 식물 기내배양기술의 이용박소영, 서미숙, 신공식, 하기훈한국자원식물학회 · 한국자원식물학회지2025 · 제38권 제5호오미자 형탁배양세포로부터 Gomisin J의 생산황성진한국약용작물학회 · 한국약용작물학회지2004 · 제12권 제6호

이 간행물의 다른 논문 (18건)

백두대간 향로봉 일대 신갈나무림의 군락구조정성철, 변준기, 천광일, 성주한pp. 1-13단근 강도에 따른 소나무 유묘의 생장 특성김재희, 나성준, 김인식, 이도형pp. 15-21Lethal temperature for Japanese pine sawyer, Monochamus alternatus, in infested wood using microwave energy김진서, 서상재pp. 33-40미강제제 처리가 밭재배 고추의 생육, 이화학적 특성, 미생물 군집 및 시들음병 방제에 미치는 영향박경미, 이용복, 곽연식, 강근혜, 이정한pp. 41-48산지전용타당성평가기준 개선을 위한 평균경사도 분석방법 비교권순덕, 김원경pp. 49-58
원문 보기