식물 추출물의 모유두세포 증식 및 모발성장 관련 단백질 발현에 미치는 영향
Effect of Plant Extracts on Hair Papilla Cell Proliferation and Hair Growth-related Protein Expression
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미녹시딜과 피나스테리드는 FDA의 승인을 받아 탈모증 치료제로 사용되고 있는 유일한 의약품이지만, 이들을 이용한 치료가 불완전하고 안전성과 잠재적 부작용에 대한 의문이 제기되면서 유사한 효과와 부작용이 적은 천연제품에 대한 관심이 높아지고 있다. 본 연구에서는 세 종류의 식물 추출물(사우팔메또 베리, 알로에 베라, 알로에 사포나리아)의 항산화 및 쥐와 사람의 모유두세포 증식을 검사하였다. 항산화 시험에선 사우팔메또 베리의 활성이 가장 높게 나타났다. 모유두세포 증식에서는 사우팔메또 베리와 알로에 사포나리아가 각각 최대 28%, 40%로 높은 성장률을 보였다. 모유두세포 증식 효능이 뛰어난 알로에 사포나리아를 용매분획하여 항산화 검사, 모유두세포 증식, 단백질 발현 분석 등을 진행하였다. 사람의 모유두세포에서 알로에 사포나리아 클로로포름 분획과 에틸 아세테이트 분획은 5 μg/mL에서 각각, 30% 및 8% 증가하였다. 쥐의 모유두세포에서 알로에 사포나리아 클로로포름 분획과 에틸 아세테이트 분획은 25 μg/mL에서 각각, 53% 및 32% 증가하였다. 부탄올 분획과 수분 분획에서 모유두세포의 유의한 증가는 없었다. 단백질 발현 분석결과, 추출물과 분획물은 AKT의 발현과 인산화에 영향을 미치는 것으로 보이지 않았으나 알로에 사포나리아 추출물, 알로에 사포나리아 클로로포름 분획, 에틸 아세테이트 분획은 ERK의 인산화를 증가시켜 모유두세포의 성장을 촉진시키는 것으로 나타났다. 알로에 사포나리아에 대한 기존 연구가 전무해 모발 성장에 효과적인 새로운 천연소재로 개발이 가능하고, 탈모 예방 및 치료에도 활용할 수 있는 것으로 확인되었다.
Abstract
Minoxidil and Finasteride are the only drugs approved by the FDA and used as treatments for alopecia, but interest in natural products with similar effects and less side effects is increasing as treatment using them is incomplete and questions about safety and potential side effects are raised. In this study, three kinds of plant extracts(Serenoa repens, Aloe saponaria, Aloe vera) were examined for antioxidant activity and, human and rat dermal papilla cells proliferation. Aomng them, Aloe saponaria was chosen as the best candidate material. For in-depth study on Aloe saponaria, which has not yet been studied, antioxidant tests, rat and dermal papilla cell proliferation, and protein expression analysis were conducted using solvent fractions. In human dermal papilla cells, Aloe saponaria extract increased cell proliferation by 28% at 25 ㎍/㎖, and in rat dermal papilla cells, it increased cell proliferation by 36% and 40% at 25 ㎍/㎖ and 50 ㎍/㎖, respectively. In human dermal papilla cells, Aloe saponaria chloroform and ethyl acetate fractions increased cell proliferation by 30% and 8% in 5 ㎍/㎖, respectively. In rat dermal papilla cells, Aloe saponaria chloroform and ethyl acetate fractions increased cell proliferation by 53% and 32% in 25 ㎍/㎖, respectively. There was no significant increase in dermal papilla cells in butanol fraction and water fraction. As a result of protein expression analysis, expression and phosphorylation of AKT were not detected, but phosphorylation of ERK was increased in Aloe saponaria extract, Aloe saponaria chloroform fraction, and Aloe saponaria ethyl acetate fraction, thereby promoting the growth of dermal papilla cells. Since there is no prior research on Aloe saponaria, it is reasonable to believe that it can be developed into a new natural substance that is effective in hair growth, hair loss prevention and treatment.
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