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인체 모유두세포의 증식에 미치는 갈근 에탄올추출물의 효과

Effect of Puerariae Radix Ethanol Extract on the Proliferation of Human Dermal Papilla Cells

우원홍|고경숙|문연자|인명희|박설아
한의병리학회 (The Society of Pathology in Korean Medicine)|2017.06|동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)|제31권 제3호|pp. 167-172|DOI http://dx.doi.org/10.15188/kjopp.2017.06.31.3.167

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키워드

ERKcell proliferationPuerariae RadixAktHHDPCs
주제분류한의학

초록

In this study, we investigated the effect of Puerariae Radix ethanol extracts (EPR). The effect of the EPR on proliferation of human hair dermal papilla cells(HHDPCs) by MTT assay and observed Expression of mechanisms that regulate cell proliferation extracellular signal-regulated kinase(ERK) and Akt by western blot. The results showed EPR increased the proliferation of HHDPCs and up-regulation phosphorylation of ERK and Akt. ERK and Akt increased by EPR inhibited phosphorylation by PD98059 (ERK inhibitor) and LY294002 (Akt inhibitor), and cell proliferation was also inhibited. These results suggested EPR increases the proliferation of HHDPCs through phosphorylation of ERK and Akt, and therefore is a beneficial effect for the alopecia treatment.

Abstract

In this study, we investigated the effect of Puerariae Radix ethanol extracts (EPR). The effect of the EPR on proliferation of human hair dermal papilla cells(HHDPCs) by MTT assay and observed Expression of mechanisms that regulate cell proliferation extracellular signal-regulated kinase(ERK) and Akt by western blot. The results showed EPR increased the proliferation of HHDPCs and up-regulation phosphorylation of ERK and Akt. ERK and Akt increased by EPR inhibited phosphorylation by PD98059 (ERK inhibitor) and LY294002 (Akt inhibitor), and cell proliferation was also inhibited. These results suggested EPR increases the proliferation of HHDPCs through phosphorylation of ERK and Akt, and therefore is a beneficial effect for the alopecia treatment.

참고문헌 (39)

  1. Mi Hee Kwack, "Preventable effect of L-threonate, an ascorbate metabolite, on androgen-driven balding via repression of dihydrotestosteroneinduced dickkopf-1 expression in human hair dermal papilla cells", BMB Reports, 43(10), pp.688~692 (2010), 생화학분자생물학회
  2. Hardy MH, "The secret life of the hair follicle", Trends Genet, 8(2), pp.55~61 (1992)
  3. Inui S, "Identification of Androgen-inducible TGF-betal Derived from Dermal Papilla Cells as a Key Mediator in Androgenetic Alopecia", J Investing Dermatol Symp Proc, 8(1), pp.69~71 (2003)
  4. Olive RF, "The Indaction of Hair Follicle Formaltion in the Aduit Hooded Rat by Vibrissa Dermal Papilla", J Embryol Exp Morphol, 23, pp.219~ (1970)
  5. Kim OY, "Effects of Ultraviolet B Irradiation and Oxidantive Stress on MicroRNA Expression Profiles in Normal Himan Dermal Papilla Cells", pp.2014
  6. Lee MJ., "Study on protective effects of epigallocatechin gallate on dihydrotestosterone-induced damage by using the microRNA expression profiles in human dermal papilla cells", pp.2015
  7. Lim NY, "Effect of fructus ligustri lucidi of H2O extract on cell proliferation and cell signal pathway in human hair dermal papilla cells", J Kor Soc Cosm, 17(4), pp.774~779 (2001)
  8. Botchkarev VA, "Molecular control of epithelial-mesenchymal interactions during hair follicle cycling", J invest Dermatol, 8(1), pp.46~55 (2003)
  9. Cho YL, "The Effect of Glycine Semen Nigra Extract on Dermal Papilla(DP)Cell Proliferation and Differentiation During Anagen Phase of Hair Cycle", J Natural Science, 22, pp.73~96 (2010)
  10. McElwee KJ, "Dietary soy oil content and soy-derived phytoestrogen genistein increase resistance to alopecia areata onset in C3H", HeJ mice, 2003, pp.12(1), 30~36
  11. Plikus M. V, "Cyclic dermal BMP Signaling Regulates Stem Cell Activation During Hair Regeneration", Nature, 451(7176), pp.340~344 (2008)
  12. 전희영, "In vitro 및 in vivo에서 검은콩 추출물의 육모 효과", 한국식품과학회지, 43(6), pp.747~753 (2011), 한국식품과학회
  13. Arase S, "Co-culture of human hair follicles and dermal papillae in a collagen matrix", J Dermatol, 17(11), pp.667~676 (1999)
  14. Zhao L, "Treatment of alopecia by transplantation of hair follicle stem cells and dermal papilla cells encapsulated in alginate gels", Med Hypothesis, 10(5), pp.1~3 (2007)
  15. Stenn KS, "Hair follicle growth controls", Dermatol. Clin, 14(4), pp.543~558 (1996)
  16. Buhl AE, "Potassium channel conductance : a mechanism affecting hair growth both in vitro and in vivo", J Invest Dermatol, 98(3), pp.315~319 (1992)
  17. Han JH, "Effect of minoxidil on proliferation and apoptosis in dermal papilla cells of human hair follicle", J Dermatol Sci, 34(2), pp.91~98 (2004)
  18. Meisheri KD, "Mechanism of action of minoxidil sulfate-induced vasodilation: a role for increased K+ permeability", J Pharmacol, 245(3), pp.751~760 (1988)
  19. Melcangi RC, "Neuroactive Steroid Levels are Modified in Cerebrospinal Fluid and Plasma of Post‐Finasteride Patients Showing Persistent Sexual Side Effects and Anxious", Depressive Symptomatology, 2013, pp.10(10), 2598~2603
  20. Hagemann T, "Positive lymphocyte transformation test in a patient with allergic contact dermatitis of the scalp after short-term use of topical minoxidil solution", Cont Derm, 53(1), pp.85~97 (2005)
  21. Lee SJ, "Bonchokungmok", pp.18~ (1980), Jinunsa
  22. 이옥희, "갈근 에탄올 추출물이 흰쥐의 항산화계에 미치는 영향", Journal of Nutrition and Health, 37(10), pp.872~880 (2004), 한국영양학회
  23. Park JM, "Cosmetic composition for reducing skin pore size and secreting sebum containing the extract of Oenothera odorata Jacq., Pueraria radix, Pine tree leaves and Ulmus pumila L", pp.2013
  24. 김일출, "갈근, 복령 및 황련의 항산화성 및 미백효과", 오일 및 응용과학 학회지, 25(2), pp.219~225 (2008), 한국유화학회
  25. 김홍배, "갈근추출액이 알레르기 염증반응에 미치는 영향", 대한한방소아과학회지, 23(3), pp.217~231 (2009), 대한한방소아과학회
  26. Jeong A Park, "Protective Effect of Puerariae Radix on Ovariectomy-induced Bone Loss in Rats", Natural Product Sciences, 17(3), pp.189~197 (2011), 한국생약학회
  27. 황성연, "갈근 추출물에 의한 염증성 Cytokine 생성 억제 및 Prostaglandin E2 활성 저해에 관한 연구", 한국식품영양과학회지, 35(1), pp.28~34 (2006), 한국식품영양과학회
  28. 윤이란, "갈근 및 녹차추출물의 아질산염 소거작용 및 간독성 보호효과", 한국가축위생학회지, 33(3), pp.275~285 (2010), 한국동물위생학회
  29. 이은, "Effects of Gal geun (Puerariae Radix) on lowering lipid and antioxidant", 한국자원식물학회지, 21(6), pp.457~461 (2008), 한국자원식물학회
  30. 이승연, "곽향과 갈근 복합제제의 대장암 세포주 HT-29 증식저해효과 및 PGE2 생성 억제효과", 생약학회지, 37(4), pp.283~289 (2006), 한국생약학회
  31. 박장순, "오레가노 추출물의 모발성장 촉진효과", 생약학회지, 44(3), pp.275~280 (2013), 한국생약학회
  32. 김종란, "산화적 스트레스 모델로써 Hydrogen peroxide에 대한 인간 모유두 세포의 유전자 발현 변화 분석", 한국미용학회지, 18(1), pp.110~119 (2012), 한국미용학회
  33. 이윤경, "검은콩과 발아 검은콩 추출물이 인간 모유두 세포 성장에 미치는 효과", 한국웰니스학회, 10(1), pp.293~301 (2015), 한국웰니스학회
  34. Shin HS, "Ginsenoside F2 Reduces Hair Loss by Controlling Apoptosis through the Sterol Regulatory Element-Binding Protein Cleavage Activating Protein and Transforming Growth Factor-β Pathways in a Dihydrotestosterone-Induced Mouse Model", Biol. Pharm. Bull, 37(5), pp.755~763 (2014)
  35. Jain R, "Avicequinone C isolated from Avicennia marina exhibits 5α-reductase-type 1 inhibitory activity using an androgenic alopecia relevant cell-based assay system", Molecules, 19(5), pp.6809~6821 (2014)
  36. 이재순, "당귀와 감초 복합 한약추출물의 모발성장 촉진효과", 대한미용학회지, 6(1), pp.49~56 (2010), 대한미용학회
  37. Hna JH, "Effect of minoxidil on proliferation and apoptosis in dermal papilla cells of human hair follicle", J Dermatol. Sci, 34(2), pp.91~98 (2004)
  38. Enshell-Seijffers D, "Beta-catenin activity in the dermal papilla regulates morphogenesis and regeneration of hair", Developmental Cell, 18(4), pp.633~642 (2010)
  39. Kiratipaiboon C, "Ciprofloxacin Improves the Stemness of Human Dermal Papilla Cells", Stem Cells Int, 16, pp.1~14 (2016)

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