Potential Control of Foodborne Pathogenic Bacteria by Pediococcus pentosaceus and Lactobacillus graminis Isolated from Fresh Vegetables
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The consumption of fresh vegetables has been related to recurrent outbreaks of foodborne diseases (FBD) worldwide. Therefore, the development of effective alternative technologies is necessary to improve the safety of these products. This study aimed to isolate and identify epiphytic lactic acid bacteria (LAB) from fresh fruits and leafy vegetables and characterize their antagonistic capacity due to their ability to produce bacteriocins or antibacterial compounds. For this, 92 LAB isolates from fruits and leafy vegetables were screened for antagonistic activity. Two strains with the highest and broadest antagonistic activities were selected for further characterization; one from cantaloupe melon (strain CM175) and one from cilantro leaves (strain C15). The cell-free supernatants (CFS) of CM175 and C15 were found to exhibit antagonistic activity against FBD-causing pathogens. The CM175 and C15 strains were identified as Pediococcus pentosaceus and Lactobacillus graminis, respectively. Notably, the P. pentosaceus CM175 CFS stopped the growth of Salmonella Typhimurium, Salmonella Saintpaul, Staphylococcus aureus, and Listeria monocytogenes, and delayed Escherichia coli O157:H7 growth. Moreover, L. graminis C15 CFS delayed the growth of all indicator pathogens, but did not completely stop it. Organic acids and bacteriocin-like molecules were determined to be possibly exerting the observed antagonistic activity of the identified LAB strains. Thus, application of the antagonistic compounds produced by Pediococcus pentosaceus and Lactobacillus graminis could be a novel and ecological strategy in developing antimicrobial biopreservatives for the food industry and mitigating FBD by reducing the biological contamination in fruit and vegetable orchards, mainly via their potential in controlling both gram-negative and gram-positive pathogenic bacteria.
Abstract
The consumption of fresh vegetables has been related to recurrent outbreaks of foodborne diseases (FBD) worldwide. Therefore, the development of effective alternative technologies is necessary to improve the safety of these products. This study aimed to isolate and identify epiphytic lactic acid bacteria (LAB) from fresh fruits and leafy vegetables and characterize their antagonistic capacity due to their ability to produce bacteriocins or antibacterial compounds. For this, 92 LAB isolates from fruits and leafy vegetables were screened for antagonistic activity. Two strains with the highest and broadest antagonistic activities were selected for further characterization; one from cantaloupe melon (strain CM175) and one from cilantro leaves (strain C15). The cell-free supernatants (CFS) of CM175 and C15 were found to exhibit antagonistic activity against FBD-causing pathogens. The CM175 and C15 strains were identified as Pediococcus pentosaceus and Lactobacillus graminis, respectively. Notably, the P. pentosaceus CM175 CFS stopped the growth of Salmonella Typhimurium, Salmonella Saintpaul, Staphylococcus aureus, and Listeria monocytogenes, and delayed Escherichia coli O157:H7 growth. Moreover, L. graminis C15 CFS delayed the growth of all indicator pathogens, but did not completely stop it. Organic acids and bacteriocin-like molecules were determined to be possibly exerting the observed antagonistic activity of the identified LAB strains. Thus, application of the antagonistic compounds produced by Pediococcus pentosaceus and Lactobacillus graminis could be a novel and ecological strategy in developing antimicrobial biopreservatives for the food industry and mitigating FBD by reducing the biological contamination in fruit and vegetable orchards, mainly via their potential in controlling both gram-negative and gram-positive pathogenic bacteria.
참고문헌 (41)
- "Food safety" (2015)[URL]
- "Foodborne germs and illnesses" (2015)[URL]
- Hussain MA, "Understanding the fresh produce safety challenges", Foods, 6, pp.23~ (2017)
- Wadamori Y, "Outbreaks and factors influencing microbiological contamination of fresh produce", J. Sci. Food Agr, 97, pp.1396~1403 (2017)
- "List of selected multistate foodborne outbreak investigations" (2018)[URL]
- "Multistate outbreak of E. coli O157:H7 infections linked to romaine lettuce (Final update)" (2018)[URL]
- "Multistate outbreak of Salmonella Typhimurium and Salmonella newport infections linked to Cantaloupe (Final update)" (2012)[URL]
- Tauxe RV, "Emerging foodborne pathogens", Int. J. Food Microbiol, 78, pp.31~41 (2002)
- Yeni F, "Most common foodborne pathogens and mycotoxins on fresh produce: a review of recent outbreaks", Crit. Rev. Food Sci. Nutr, 56, pp.1532~1544 (2016)
- Janisiewicz WJ, "Biological control of postharvest diseases of fruits", Annu. Rev. Phytopathol, 40, pp.411~441 (2002)
- Jordan K, "Microbes versus microbes: control of pathogens in the food chain", J. Sci. Food Agr, 94, pp.3079~3089 (2014)
- González-Pérez CJ, "Induction of bacteriocins from lactic acid bacteria; a strategy to improve the safety of fresh fruits and vegetables", ARTOAJ, 14, pp.555927~ (2018)
- Karpiński TM, "Characteristic of bacteriocines and their application", Pol. J. Microbiol, 62, pp.223~235 (2013)
- Alvarez-Sieiro P, "Bacteriocins of lactic acid bacteria: extending the family", Appl. Microbiol. Biotechnol, 100, pp.2939~2951 (2016)
- Barbosa AAT, "Bacteriocins from lactic acid bacteria and their potential in the preservation of fruit products", Crit. Rev. Biotechnol, 37, pp.852~864 (2017)
- Lubelski J, "Biosynthesis, immunity, regulation, mode of action and engineering of the model lantibiotic nisin", Cell. Mol. Life Sci, 65, pp.455~476 (2008)
- Woraprayote W, "Bacteriocins from lactic acid bacteria and their applications in meat and meat products", Meat Sci, 120, pp.118~132 (2016)
- Ahmadova A, "Antimicrobial and antifungal activities of Lactobacillus curvatus strain isolated from homemade Azerbaijani cheese", Anaerobe, 20, pp.42~49 (2013)
- Shokryazdan P, "Probiotic potential of Lactobacillus strains with antimicrobial activity against some human pathogenic strains", BioMed. Res. Int, pp.927268~ (2014)
- Todorov SD, "Bacteriocin production by Pediococcus pentosaceus isolated from marula (Scerocarya birrea)", Int. J. Food Microbiol, 132, pp.117~126 (2009)
- Yi L, "Purification, characterization and bactericidal mechanism of a broad spectrum bacteriocin with antimicrobial activity against multidrug-resistant strains produced by Lactobacillus coryniformis XN8", Food Control, 67, pp.53~62 (2016)
- Bin Shan, "Potential Application of Spice and Herb Extracts as Natural Preservatives in Cheese", Journal of Medicinal Food, 14(3), pp.284~290 (2011), 한국식품영양과학회
- Hernandez D, "Antimicrobial activity of lactic acid bacteria isolated from Tenerife cheese: initial characterization of plantaricin TF711, a bacteriocin‐like substance produced by Lactobacillus plantarum TF711", Microbiol, 99, pp.77~84 (2005)
- de Lima Marques J, "Bacteriocin-like substances of Lactobacillus curvatus P99: characterization and application in biodegradable films for control of Listeria monocytogenes in cheese", Food Microbiol, 63, pp.159~163 (2017)
- Hall BG, "Growth rates made easy", Mol. Biol. Evol, 31, pp.232~238 (2014)
- Doi K, "Pediococcus lolii sp. nov., isolated from ryegrass silage. Int. J. Syst", Evol. Microbiol, 59, pp.1007~1010 (2009)
- Cruz‐Guerrero A, "Commercial probiotic bacteria and prebiotic carbohydrates: a fundamental study on prebiotics uptake, antimicrobials production and inhibition of pathogens", J. Sci. Food Agr, 94, pp.2246~2252 (2014)
- Macwana SJ, "A ‘bacteriocin PCR array’ for identification of bacteriocin-related structural genes in lactic acid bacteria", J. Microbiol. Methods, 88, pp.197~204 (2012)
- Savadogo A, "Bacteriocins and lactic acid bacteria-a minireview", Afr. J. Biotechnol, 5, pp.678~683 (2006)
- Leff JW, "Bacterial communities associated with the surfaces of fresh fruits and vegetables", PLoS One, 8, pp.e59310~ (2013)
- Corsetti A, "taxonomic survey of lactic acid bacteria isolated from wheat (Triticum durum) kernels and non-conventional flours", Syst. Appl. Microbiol, 30, pp.561~571 (2007)
- Beck R, "Lactobacillus graminis sp. nov., a new species of facultatively heterofermentative lactobacilli surviving at low pH in grass silage", Syst. Appl. Microbiol, 10, pp.279~283 (1988)
- Albano H, "Characterization of two bacteriocins produced by Pediococcus acidilactici isolated from “Alheira”, a fermented sausage traditionally produced in Portugal", Int. J. Food Microbiol, 116, pp.239~247 (2007)
- Maldonado-Barragán A, "Purification and genetic characterization of gassericin E, a novel co-culture inducible bacteriocin from Lactobacillus gasseri EV1461 isolated from the vagina of a healthy woman", BMC Microbiol, 16, pp.37~ (2016)
- Hayek SA, "Current limitations and challenges with lactic acid bacteria: a review", Food Nutr. Sci, 4, pp.73~87 (2013)
- Vijayakumar PP, "A microplate growth inhibition assay for screening bacteriocins against Listeria monocytogenes to differentiate their mode-of-action", Biomolecules, 5, pp.1178~1194 (2015)
- Ovchinnikov KV, "Novel group of leaderless multipeptide bacteriocins from grampositive bacteria", Appl. Environ. Microbiol, 82, pp.5216~5224 (2016)
- Von Mollendorff J, "Comparison of bacteriocins produced by lactic-acid bacteria isolated from boza, a cereal-based fermented beverage from the Balkan Peninsula", Curr. Microbiol, 53, pp.209~216 (2006)
- Todorov SD, "Safety of Lactobacillus plantarum ST8Sh and its bacteriocin", Proteins, 9, pp.334~344 (2017)
- Muhialdin BJ, "Antifungal activity of Lactobacillus fermentum Te007, Pediococcus pentosaceus Te010, Lactobacillus pentosus G004, and L. paracasi D5 on selected foods", J. Food Sci, 76, pp.M493~M499 (2011)
- Chikindas ML, "Functions and emerging applications of bacteriocins", Curr. Opin. Biotechnol, 49, pp.23~28 (2018)